All minpreps from yesterday digested with EcoRI and PstI
Each lane is a separate miniprep (2 minipreps of each transformation).
Band from the EpsE digests is approximately correctly, although this is also approxiamtely the size of GFP-Termiantor and RFP-Terminator. In particular, the pSB1AK3 vector containing RFP-T was used to provide vector for the ligation.
All other mini-preps do not appear to have inserts! The gel had been imaged earlier and had also shown no evidence of inserts.
It should also be noted that the vector in all the minipreps appears to be the wrong size!
Dry Lab
Modelling Motility
Done up the code to model the cell's trajectory using least square curve fit method.
Fitted cell trajectories of 100908 Video 15. Results look promising:
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