Imperial College/24 September 2008
From 2008.igem.org
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*All the mini-preps were digested with ''Eco''RI and ''Pst''I | *All the mini-preps were digested with ''Eco''RI and ''Pst''I | ||
- | [[Image:24-9minidig.png|center| | + | [[Image:24-9minidig.png|thumb|center|700px| U = undigested, D = digested]] |
*All digested bands appear to be approximately the right size, no trace of contamiantion beyond occaisonal bits of denatured DNA | *All digested bands appear to be approximately the right size, no trace of contamiantion beyond occaisonal bits of denatured DNA | ||
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+ | *Minipreps of PgsiB-gsiB, PgsiB-spoVG and Pveg-spoVG were digested with ''Eco''RI and ''Pst''I and run on a gel along with uncut DNA from the minipreps of GeneArt constructs 4 and 5 | ||
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+ | [[Image:24-9minidig2.png|thumb|center|700px| U = undigested, D = digested]] | ||
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+ | *The sizes of inserts for PgsiB-gsiB and PgsiB-spoVG appear correct and the mini-preps seem fairly pure. No insert was cut from the Pveg-spoVG minipreps and they will need to be re-ligated for transformation | ||
==Dry Lab== | ==Dry Lab== |
Revision as of 13:46, 26 September 2008
24 September 2008Wet LabMiniprep
Miniprep Digestion
Dry LabModelling Motility
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