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Imperial College/2 October 2008
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==Wetlab== | ==Wetlab== | ||
*The ligations of various PCR products into AK3 were transformed and single colony PCR was carried out to check for correct ligation. The following PCR products were ligated: | *The ligations of various PCR products into AK3 were transformed and single colony PCR was carried out to check for correct ligation. The following PCR products were ligated: | ||
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**1 cycle - 95<sup>o</sup>C for 30 sec | **1 cycle - 95<sup>o</sup>C for 30 sec | ||
**30 cycle - 95<sup>o</sup>C for 30 sec, 60<sup>o</sup>C for 45 sec, 72<sup>o</sup>C for 1 min | **30 cycle - 95<sup>o</sup>C for 30 sec, 60<sup>o</sup>C for 45 sec, 72<sup>o</sup>C for 1 min | ||
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[[Image:2octscp.PNG|center|400px]] | [[Image:2octscp.PNG|center|400px]] | ||
*The following colonies appear to have correct inserts: 5'E1, 3'E2, Spec1 | *The following colonies appear to have correct inserts: 5'E1, 3'E2, Spec1 | ||
Revision as of 19:06, 15 October 2008
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2 October 2008Wetlab
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