Imperial College/9 October 2008
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==Wetlab== | ==Wetlab== | ||
*The ligations of various parts were transformed and single colony PCR was carried out to check for correct ligation. The following parts that were ligated were: | *The ligations of various parts were transformed and single colony PCR was carried out to check for correct ligation. The following parts that were ligated were: | ||
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**1 cycle - 95<sup>o</sup>C for 30 sec | **1 cycle - 95<sup>o</sup>C for 30 sec | ||
**30 cycle - 95<sup>o</sup>C for 30 sec, 60<sup>o</sup>C for 45 sec, 72<sup>o</sup>C for 1 min | **30 cycle - 95<sup>o</sup>C for 30 sec, 60<sup>o</sup>C for 45 sec, 72<sup>o</sup>C for 1 min | ||
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[[Image:9thoCT.PNG|center|400px]] | [[Image:9thoCT.PNG|center|400px]] | ||
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*All ligations appear to have worked and now midi preps will be prepared. | *All ligations appear to have worked and now midi preps will be prepared. | ||
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+ | {{Imperial/EndPage|Notebook|Notebook}} |
Latest revision as of 20:51, 28 October 2008
9 October 2008Wetlab
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