Imperial College/9 October 2008

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**1 cycle - 95<sup>o</sup>C for 30 sec
**1 cycle - 95<sup>o</sup>C for 30 sec
**30 cycle - 95<sup>o</sup>C for 30 sec, 60<sup>o</sup>C for 45 sec, 72<sup>o</sup>C for 1 min
**30 cycle - 95<sup>o</sup>C for 30 sec, 60<sup>o</sup>C for 45 sec, 72<sup>o</sup>C for 1 min
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[[Image:9thoCT.PNG|center|400px]]
[[Image:9thoCT.PNG|center|400px]]
*All ligations appear to have worked and now midi preps will be prepared.
*All ligations appear to have worked and now midi preps will be prepared.
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{{Imperial/EndPage|Notebook|Notebook}}

Latest revision as of 20:51, 28 October 2008

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9 October 2008

Wetlab

  • The ligations of various parts were transformed and single colony PCR was carried out to check for correct ligation. The following parts that were ligated were:
    • 3'AmyE + GFP-T
    • 3'AmyE + RFP-T
    • 6L(LipA-Elastin)
  • The pSB primers were used on the following cycle:
    • 1 cycle - 95oC for 30 sec
    • 30 cycle - 95oC for 30 sec, 60oC for 45 sec, 72oC for 1 min


9thoCT.PNG
  • All ligations appear to have worked and now midi preps will be prepared.



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