The ligations of various parts were transformed and single colony PCR was carried out to check for correct ligation. The following parts that were ligated were:
3'AmyE + GFP-T
3'AmyE + RFP-T
6L(LipA-Elastin)
The pSB primers were used on the following cycle:
1 cycle - 95oC for 30 sec
30 cycle - 95oC for 30 sec, 60oC for 45 sec, 72oC for 1 min
All ligations appear to have worked and now midi preps will be prepared.