9 October 2008
Wetlab
- The ligations of various parts were transformed and single colony PCR was carried out to check for correct ligation. The following parts that were ligated were:
- 3'AmyE + GFP-T
- 3'AmyE + RFP-T
- 6L(LipA-Elastin)
- The pSB primers were used on the following cycle:
- 1 cycle - 95oC for 30 sec
- 30 cycle - 95oC for 30 sec, 60oC for 45 sec, 72oC for 1 min
- All ligations appear to have worked and now midi preps will be prepared.
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