User contributions
From 2008.igem.org
(Latest | Earliest) View (newer 500 | older 500) (20 | 50 | 100 | 250 | 500)
- 17:40, 28 October 2008 (diff | hist) N File:McConkey Plate 4.JPG (top)
- 17:40, 28 October 2008 (diff | hist) N File:McConkey Plate 2.JPG (top)
- 17:39, 28 October 2008 (diff | hist) N File:0627 digest TA.jpg (top)
- 17:39, 28 October 2008 (diff | hist) File:16s PCR Gel2(MR1 + Ecoli).jpg (uploaded a new version of "Image:16s PCR Gel2(MR1 + Ecoli).jpg") (top)
- 17:39, 28 October 2008 (diff | hist) File:16s PCR Gel1(S1).jpg (uploaded a new version of "Image:16s PCR Gel1(S1).jpg") (top)
- 17:39, 28 October 2008 (diff | hist) File:6-28 mxh 5.jpg (uploaded a new version of "Image:6-28 mxh 5.jpg") (top)
- 17:39, 28 October 2008 (diff | hist) File:6-28 mxh 4.jpg (uploaded a new version of "Image:6-28 mxh 4.jpg") (top)
- 17:39, 28 October 2008 (diff | hist) File:6-28 mxh 3.jpg (uploaded a new version of "Image:6-28 mxh 3.jpg") (top)
- 17:39, 28 October 2008 (diff | hist) File:6-28 mxh 2.jpg (uploaded a new version of "Image:6-28 mxh 2.jpg") (top)
- 17:39, 28 October 2008 (diff | hist) File:6-28 mxh 1.jpg (uploaded a new version of "Image:6-28 mxh 1.jpg") (top)
- 17:39, 28 October 2008 (diff | hist) File:6-27 redigest TA.jpg (uploaded a new version of "Image:6-27 redigest TA.jpg") (top)
- 17:38, 28 October 2008 (diff | hist) File:6-25 digest.jpg (uploaded a new version of "Image:6-25 digest.jpg") (top)
- 17:34, 28 October 2008 (diff | hist) Team:Harvard/Dailybook/Week0 (top)
- 17:33, 28 October 2008 (diff | hist) Team:Harvard/Dailybook/Week0
- 17:32, 28 October 2008 (diff | hist) Team:Harvard/Dailybook/Week0
- 23:19, 26 October 2008 (diff | hist) Team:Harvard/Notebook
- 23:19, 26 October 2008 (diff | hist) Team:Harvard/Notebook
- 19:54, 26 October 2008 (diff | hist) N File:16s PCR Gel2(MR1 + Ecoli).jpg
- 19:54, 26 October 2008 (diff | hist) N File:16s PCR Gel1(S1).jpg
- 19:54, 26 October 2008 (diff | hist) N File:6-28 mxh 5.jpg
- 19:54, 26 October 2008 (diff | hist) N File:6-28 mxh 4.jpg
- 19:53, 26 October 2008 (diff | hist) N File:6-28 mxh 3.jpg
- 19:53, 26 October 2008 (diff | hist) N File:6-28 mxh 2.jpg
- 19:53, 26 October 2008 (diff | hist) N File:6-28 mxh 1.jpg
- 19:53, 26 October 2008 (diff | hist) N File:6-27 redigest TA.jpg
- 19:53, 26 October 2008 (diff | hist) N File:6-25 digest.jpg
- 19:49, 26 October 2008 (diff | hist) Team:Harvard/Dailybook/Week0 (→Transformed S1 via Electroporation)
- 19:48, 26 October 2008 (diff | hist) Team:Harvard/Dailybook/Week0 (→Transformed S1 via Electroporation)
- 19:48, 26 October 2008 (diff | hist) Team:Harvard/Dailybook/Week0 (→Transformed S1 via Electroporation)
- 19:47, 26 October 2008 (diff | hist) Team:Harvard/Dailybook/Week0 (→Protocols)
- 19:47, 26 October 2008 (diff | hist) Team:Harvard/Dailybook/Week0 (→Tuesday: June 17, 2008)
- 19:47, 26 October 2008 (diff | hist) Team:Harvard/Dailybook/Week0 (→Tuesday: June 17, 2008)
- 19:45, 26 October 2008 (diff | hist) N File:PACYC-map.pdf (top)
- 19:44, 26 October 2008 (diff | hist) N Team:Harvard/Plasmids (New page: == Vectors == *pACYC-Duet vector map *[http://partsregistry.org/wiki/index.php?title=Part:pSB3K3 pSB3K3] ==Parts== *[http://spreadsheets.google.com/ccc?key=pwvx...)
- 19:44, 26 October 2008 (diff | hist) Team:Harvard/Notebook (→Notebooks)
- 19:42, 26 October 2008 (diff | hist) Team:Harvard/Team
- 19:41, 26 October 2008 (diff | hist) Team:Harvard/Team (→Advisors)
- 19:40, 26 October 2008 (diff | hist) Team:Harvard/Team (→Teaching Fellows)
- 19:40, 26 October 2008 (diff | hist) Team:Harvard/Team
- 19:39, 26 October 2008 (diff | hist) Team:Harvard/Team
- 19:35, 26 October 2008 (diff | hist) Team:Harvard/Notebook (→Notebooks)
- 19:35, 26 October 2008 (diff | hist) N Team:Harvard/Recipes (New page: =Recipes= ==Orianna's growth media== * 50 mM K-PIPES '''18.925 g/L''' * 7.5 mM NaOH '''7.5 mL/L''' * 28 mM NH<sub>4</sub>Cl '''1.5 g/L''' * 1.3 mM KCl ...)
- 19:34, 26 October 2008 (diff | hist) N Team:Harvard/PrelimTest (New page: =Week 0= ==OD and GFP expression in ''E. Coli''== June 17, 2008 ===3 hrs in Incubation=== {| {{table}} | align="center" style="background:#eeeeee;"|'''Cell Type''' | align="center" style=...) (top)
- 19:32, 26 October 2008 (diff | hist) N Team:Harvard/MFCProtocols (New page: =Running an MFC Experiment from Start to Finish= This page is intended as a comprehensive guide to completing a microbial fuel cell experiment ==Creating a Testing Environment== Begin ...) (top)
- 19:32, 26 October 2008 (diff | hist) Team:Harvard/Notebook
- 19:31, 26 October 2008 (diff | hist) N MFCProtocols (New page: =Running an MFC Experiment from Start to Finish= This page is intended as a comprehensive guide to completing a microbial fuel cell experiment ==Creating a Testing Environment== Begin ...) (top)
- 19:26, 26 October 2008 (diff | hist) N Team:Harvard/GenProtocols (New page: ==QIAprep Spin Miniprep Kit== This protocol is designed for the purification of up to 20 ug high-copy plasmid DNA from 1-5 ml overnight ''E. coli'' culture in LB medium. ===Procedure=== #...)
- 19:26, 26 October 2008 (diff | hist) Team:Harvard/Notebook
- 19:24, 26 October 2008 (diff | hist) N GenProtocols (New page: ==QIAprep Spin Miniprep Kit== This protocol is designed for the purification of up to 20 ug high-copy plasmid DNA from 1-5 ml overnight ''E. coli'' culture in LB medium. ===Procedure=== #...) (top)
- 19:22, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week14/Chemical and Light (New page: =Mystery Colony PCR 10/08= Colony PCR of putative fungal colonies using primers BBpfx and mtrB500. Image:10-08_Mystery_PCR_MXHTA.jpg Lane 1: 1 kb ladder Lanes 2-6: P136 plate (500 b...) (top)
- 19:22, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week13/Chemical and Light (New page: =Colony PCR 09/30= Should grow only on carb *1-30: 20, 23, 25 (picked all three) --> P38+130 *31-60: 31-60 (picked 31, 35, 39, 42, 46, 50, 55, 60) --> P39+130 Should grow only on kan *61-...) (top)
- 19:22, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week12/Chemical and Light (New page: =9/22 RE digests= With NEB enzymes, 60 min (buffer 2+BSA): *128#1 XPXmnI *128#5 XSXmnI *128#9 ESXmnI The XmnI is to cut the backbone, which has a similar length to the mtrB constructs. W...)
- 19:21, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week11/Chemical and Light (New page: <b>Summary of the week</b> * Retransformed plasmids containing low expression ompR and HO-pcyA (for light system) w/ Cm resistance * Ligated mtrB after an RBS and before a terminator (2 se...) (top)
- 19:21, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week10/Chemical and Light (New page: =Transformations in Shewie= About 250 ng of DNA added each time. ==First Set 8/22== {| {{table}} | align="center" style="background:#f0f0f0;"|'''Plate''' | align="center" style="backgrou...) (top)
- 19:20, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week9/Chemical and Light (New page: =105, mtrB Ligations/transformations 08/17= Parts to ligated came from 8/17 We used a 2:1 molar ratio of insert to vector for all of the ligations. The ligations were all transformed into...) (top)
- 19:20, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week8/Chemical and Light (New page: =MIT lacZ parts RE digests 08/11= {| {{table}} !rowspan=8|Image:8-11_gel_1_MXHTA.jpg !colspan=2 align="center" style="background:#f0f0f0;"|'''1% agarose''' |- | align="center" style="b...) (top)
- 19:19, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week7/Widgetry (New page: =Goals for this week= =Monday: August 4, 2008= =Tuesday: August 5, 2008= =Wednesday: August 6, 2008= =Thursday: August 7, 2008= =Friday: August 8, 2008=)
- 19:19, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week7/Chemical and Light (New page: =PCR= ==8/4: Cph/EnvZ, P1, P3, mtrB not BB== {| {{table}} !rowspan=9|Image:8-04_gel 1_MXHTA.jpg !colspan=2 align="center" style="background:#f0f0f0;"|'''1% agarose gel visualized usin...) (top)
- 19:19, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week6/Widgetry (New page: =Goals for this week= =Monday: July 28, 2008= =Tuesday: July 29, 2008= =Wednesday: July 30, 2008= =Thursday: July 31, 2008= =Friday: August 1, 2008=)
- 19:18, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week6/Chemical and Light (New page: =Sequencing Parts= =Making Thermoinducible cI System = ==Minipreps from Transformations of P75 + P63 7/28== {| {{table}} | align="center" style="background:#f0f0f0;"|'''Sample ID''' | alig...) (top)
- 19:05, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week5/Widgetry (New page: =Monday: July 21, 2008= *Lab meeting *Cleaned chambers =Tuesday: July 22, 2008= *started mtrB and wt cultures *troubleshoot LabVIEW =Wednesday: July 23, 2008= =Thursday: July 24, 2008= =...)
- 19:03, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week5/Chemical and Light (New page: =Testing Thermoinducible Lac System= ==Restreaked Plates of P92-3 in S1 7/21== Restreaked P93 (1:3), P92a, and P93b--all successfully transformed in S1--to detect YFP at 30 and 40 degrees....) (top)
- 19:03, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week4/Widgetry (New page: =Monday: July 14, 2008= * Built three more fuel cell chambers * Ran three wildtype MFC's 600px '''Conclusion:''' *Perhaps cells have died. *Produced negligible a...) (top)
- 19:03, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week4/Chemical and Light (New page: =PCR/primers= ==HO+pcyA, P3 vector w/o GFP== Primers were designed to PCR out the heme oxygenase and pcyA coding regions from the UT Austin plasmids. Due to the RE sites in these nonBB pla...) (top)
- 19:02, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week3/Widgetry (New page: =Goals for this week= #Make Orianna's growth media #construct more chambers to run parallel tests #set up Keithley for multiple tests #grow mtrA, mtrB,wt, E. coli in liquid cultures #negat...)
- 19:02, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week3/Chemical and Light (New page: =Goals for Week 3= =[http://openwetware.org/images/d/dd/7-7monmtg_slides_%28compatible%29.ppt Summary Powerpoint by Amy]= =The Cloning Strategy= ==Goals:== *<b>Lac Plasmid</b>: Hi/low p...) (top)
- 19:01, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week14 (New page: Chemical and Light<br> Widgetry<br>)
- 19:01, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week13 (New page: Chemical and Light<br> Widgetry<br>)
- 19:01, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week12 (New page: Chemical and Light<br> Widgetry<br>) (top)
- 19:01, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week11 (New page: Chemical and Light<br> Widgetry<br>)
- 19:01, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week10 (New page: Chemical and Light<br> Widgetry<br>)
- 19:01, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week9 (New page: Chemical and Light<br> Widgetry<br>) (top)
- 19:00, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week8 (New page: Chemical and Light<br> Widgetry<br>) (top)
- 19:00, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week7 (New page: Chemical and Light<br> Widgetry<br>) (top)
- 19:00, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week6 (New page: Chemical and Light<br> Widgetry<br>) (top)
- 19:00, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week5 (New page: Chemical and Light<br> Widgetry<br>) (top)
- 19:00, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week4 (New page: Chemical and Light<br> Widgetry<br>) (top)
- 19:00, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week3 (New page: Chemical and Light<br> Widgetry<br>) (top)
- 18:57, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week2/Widgetry (New page: =Goals for this week= #Talk to Helen, Pete, etc. =) #Work on growing anaerobically, the electric current, and everything together? #Check that Shewie is growing by putting it with media, b...) (top)
- 18:56, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week2/Chemical and Light (New page: =Goals for Week 2= #Transform LacI, TetR, cI + their promoters and GFP. Refer http://partsregistry.org/Part:BBa_Q04121 here. #Cut Lac promoter -> GFP (P20), and Tet promoter -> GFP (P...) (top)
- 18:56, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week2 (New page: Chemical and Light<br> Widgetry<br>) (top)
- 18:55, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week1/Widgetry (New page: =Monday: June 23, 2008= ==Lab== * Received ordered parts from McMaster-Carr * Drew preliminary designs for MFC 300px * Cut polycarbonate tubing ** 2" Square T...)
- 18:54, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week1/Chemical and Light (New page: =Chemical= ==Results from Friday (6/20) Electroporation and Transformation in S1== ===6/23:=== :::{| {{table}} | align="center" style="background:#eeeeee;"|'''Name''' | align="center" styl...) (top)
- 18:53, 26 October 2008 (diff | hist) Team:Harvard/Dailybook/Week1 (top)
- 18:53, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week1 (New page: Team:Harvard/Dailybook/Week1/Chemical and Light<br> Team:Harvard/Dailybook/Week1/Widgetry<br> =Monday Meeting Notes= == Goals for Week 1 == === Light team=== * knockout protocol ...)
- 18:50, 26 October 2008 (diff | hist) Team:Harvard/Dailybook/Week0
- 18:49, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week0 (New page: {| | align="center" style="background:#c4dbea"| <br><br> <html><a href = "https://2008.igem.org/Team:Harvard"><img src="https://static.igem.org/mediawiki/2008/b/b9/Harvard_logo.png"></a></html> {...)
- 18:49, 26 October 2008 (diff | hist) Team:Harvard/DailyBook
- 18:45, 26 October 2008 (diff | hist) Nm Team:Harvard/DailyBook (New page: {| | align="center" style="background:#c4dbea"| <br><br> <html><a href = "https://2008.igem.org/Team:Harvard"><img src="https://static.igem.org/mediawiki/2008/b/b9/Harvard_logo.png"></a></html> {...)
- 18:45, 26 October 2008 (diff | hist) Team:Harvard/Notebook (→Notebooks)
- 18:41, 26 October 2008 (diff | hist) Team:Harvard/Notebook
- 18:41, 26 October 2008 (diff | hist) N DailyBook (New page: {| | align="center" style="background:#c4dbea"| <br><br> <html><a href = "https://2008.igem.org/Team:Harvard"><img src="https://static.igem.org/mediawiki/2008/b/b9/Harvard_logo.png"></a></html> {...) (top)
- 18:40, 26 October 2008 (diff | hist) Team:Harvard/Notebook
- 18:40, 26 October 2008 (diff | hist) Team:Harvard/Notebook
- 18:39, 26 October 2008 (diff | hist) N /DailyBook (New page: {| | align="center" style="background:#c4dbea"| <br><br> <html><a href = "https://2008.igem.org/Team:Harvard"><img src="https://static.igem.org/mediawiki/2008/b/b9/Harvard_logo.png"></a></html> {...) (top)
- 18:38, 26 October 2008 (diff | hist) Team:Harvard/Notebook (→Notebooks)
- 18:38, 26 October 2008 (diff | hist) N /DailyBook/ (New page: *Week 0 (6/16-6/22) *Week 1 (6/23-6/29) *Week 2 (6/30-7/6) *Week 3 (7/7-7/13) *Week 4 (7/14-7/20) *[[/Week5/|Week 5 (7/21-7/27)]...) (top)
- 17:46, 26 October 2008 (diff | hist) Team:Harvard/Notebook
- 17:44, 26 October 2008 (diff | hist) Team:Harvard/Notebook
- 17:43, 26 October 2008 (diff | hist) Team:Harvard/Notebook
- 17:42, 26 October 2008 (diff | hist) Team:Harvard/Notebook
- 17:42, 26 October 2008 (diff | hist) Team:Harvard/Modeling (top)
- 17:40, 26 October 2008 (diff | hist) Team:Harvard/Notebook
- 17:40, 26 October 2008 (diff | hist) Team:Harvard/Modeling
- 17:39, 26 October 2008 (diff | hist) Team:Harvard/Parts
- 17:38, 26 October 2008 (diff | hist) Team:Harvard/Parts
- 17:37, 26 October 2008 (diff | hist) Team:Harvard/Parts
- 17:36, 26 October 2008 (diff | hist) Team:Harvard/Project
- 17:35, 26 October 2008 (diff | hist) Team:Harvard/Project
- 03:22, 26 October 2008 (diff | hist) Team:Harvard/Team
- 07:06, 24 October 2008 (diff | hist) Team:Harvard/Team
- 06:57, 24 October 2008 (diff | hist) Team:Harvard/Team
- 06:49, 24 October 2008 (diff | hist) Team:Harvard/Team
- 06:33, 24 October 2008 (diff | hist) Team:Harvard
- 06:33, 24 October 2008 (diff | hist) Team:Harvard
- 06:32, 24 October 2008 (diff | hist) Team:Harvard
- 06:32, 24 October 2008 (diff | hist) Team:Harvard
- 06:31, 24 October 2008 (diff | hist) Team:Harvard
- 06:31, 24 October 2008 (diff | hist) File:Harvard logo.png (uploaded a new version of "Image:Harvard logo.png") (top)
- 06:26, 24 October 2008 (diff | hist) Team:Harvard
- 06:22, 24 October 2008 (diff | hist) Team:Harvard
- 06:21, 24 October 2008 (diff | hist) Team:Harvard
- 06:21, 24 October 2008 (diff | hist) File:HModelling2.jpg (uploaded a new version of "Image:HModelling2.jpg") (top)
- 06:21, 24 October 2008 (diff | hist) File:HModelling1.jpg (uploaded a new version of "Image:HModelling1.jpg") (top)
- 06:05, 24 October 2008 (diff | hist) Team:Harvard
- 06:05, 24 October 2008 (diff | hist) Team:Harvard
- 06:05, 24 October 2008 (diff | hist) Team:Harvard
- 06:04, 24 October 2008 (diff | hist) Team:Harvard
- 20:50, 22 October 2008 (diff | hist) N File:HTeam1.jpg (top)
- 20:49, 22 October 2008 (diff | hist) N File:HTeam2.jpg (top)
- 20:49, 22 October 2008 (diff | hist) N File:HRegistry1.jpg (top)
- 20:49, 22 October 2008 (diff | hist) N File:HRegistry2.jpg (top)
- 20:48, 22 October 2008 (diff | hist) N File:HProject1.jpg (top)
- 20:48, 22 October 2008 (diff | hist) N File:HProject2.jpg (top)
- 20:48, 22 October 2008 (diff | hist) N File:HNotebook.jpg (top)
- 20:48, 22 October 2008 (diff | hist) N File:HNotebook2.jpg (top)
- 20:47, 22 October 2008 (diff | hist) N File:HModelling1.jpg
- 20:47, 22 October 2008 (diff | hist) N File:HModelling2.jpg
- 20:47, 22 October 2008 (diff | hist) N File:HHome2.jpg (top)
- 20:47, 22 October 2008 (diff | hist) N File:HHome1.jpg (top)
- 20:04, 22 October 2008 (diff | hist) Team:Harvard
- 19:47, 22 October 2008 (diff | hist) Team:Harvard
- 07:41, 22 October 2008 (diff | hist) Team:Harvard
- 07:40, 22 October 2008 (diff | hist) Team:Harvard
- 07:34, 22 October 2008 (diff | hist) Team:Harvard
- 07:29, 22 October 2008 (diff | hist) Team:Harvard
- 07:29, 22 October 2008 (diff | hist) Team:Harvard
- 07:28, 22 October 2008 (diff | hist) Team:Harvard
- 07:09, 22 October 2008 (diff | hist) Team:Harvard
- 07:09, 22 October 2008 (diff | hist) File:Harvard logo.png (uploaded a new version of "Image:Harvard logo.png")
(Latest | Earliest) View (newer 500 | older 500) (20 | 50 | 100 | 250 | 500)