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- 22:08, 29 October 2008 (diff | hist) Team:Hawaii/Parts (→Parts in process (building))
- 22:04, 29 October 2008 (diff | hist) Team:Hawaii/Parts (→Parts in process (building))
- 21:51, 29 October 2008 (diff | hist) Team:Hawaii/Project (→Part A: Mobilizable Broad-Host-Range Plasmid)
- 21:43, 29 October 2008 (diff | hist) Team:Hawaii/Antibiotic test for BB-pRL1383a (→Results)
- 20:11, 29 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-29 (→Antibiotic Test, round 2)
- 20:10, 29 October 2008 (diff | hist) Team:Hawaii/Antibiotic test for BB-pRL1383a (→Discussion)
- 20:09, 29 October 2008 (diff | hist) Team:Hawaii/Antibiotic test for BB-pRL1383a (→Discussion)
- 20:08, 29 October 2008 (diff | hist) Team:Hawaii/Antibiotic test for BB-pRL1383a (→Results)
- 20:07, 29 October 2008 (diff | hist) Team:Hawaii/Antibiotic test for BB-pRL1383a (→Results)
- 20:02, 29 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-29 (→Antibiotic Test, round 2)
- 20:02, 29 October 2008 (diff | hist) N Team:Hawaii/Notebook/2008-10-29 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Antibiotic Test, round 2=== :<strong> Grace</strong> {|class=wikitable...)
- 19:54, 29 October 2008 (diff | hist) Team:Hawaii/Project/Part B MaterialsMethodsResults (→Step 3: Conversion of pRL1383a into a Biobrick plasmid)
- 19:54, 29 October 2008 (diff | hist) Team:Hawaii/Project/Part B MaterialsMethodsResults (→Step 3: Conversion of pRL1383a into a Biobrick plasmid)
- 19:49, 29 October 2008 (diff | hist) Team:Hawaii/Project/Part B MaterialsMethodsResults (→Step 4: Device construction)
- 19:47, 29 October 2008 (diff | hist) Team:Hawaii/Project/Part B MaterialsMethodsResults (→Step 3: Conversion of pRL1383a into a Biobrick plasmid)
- 19:43, 29 October 2008 (diff | hist) Team:Hawaii/Project/Part B MaterialsMethodsResults (→Step 4: Device construction)
- 19:43, 29 October 2008 (diff | hist) Team:Hawaii/Project/Part B MaterialsMethodsResults (→Step 4: Device construction)
- 19:43, 29 October 2008 (diff | hist) Team:Hawaii/Project/Part B MaterialsMethodsResults (→Step 3: Conversion of pRL1383a into a Biobrick plasmid)
- 19:42, 29 October 2008 (diff | hist) Team:Hawaii/Project/Part B MaterialsMethodsResults (→Step 2: Site-directed mutagenesis of GFP (BBa_E0040) into a fusion brick)
- 19:42, 29 October 2008 (diff | hist) Team:Hawaii/Project/Part B MaterialsMethodsResults (→Step 3: Conversion of pRL1383a into a Biobrick plasmid)
- 19:41, 29 October 2008 (diff | hist) Team:Hawaii/Project/Part B MaterialsMethodsResults (→Step 3: Conversion of pRL1383a into a Biobrick plasmid)
- 19:40, 29 October 2008 (diff | hist) N File:BB1sequence.jpg (top)
- 19:40, 29 October 2008 (diff | hist) Team:Hawaii/Project/Part B MaterialsMethodsResults (→Step 3: Conversion of pRL1383a into a Biobrick plasmid)
- 19:20, 29 October 2008 (diff | hist) Team:Hawaii/Project/Part B MaterialsMethodsResults (→Step 4: Device construction)
- 19:18, 29 October 2008 (diff | hist) N File:102808glowingprsgecoli.jpg (top)
- 19:16, 29 October 2008 (diff | hist) Team:Hawaii/Project/Part B MaterialsMethodsResults (→Step 4: Device construction)
- 19:15, 29 October 2008 (diff | hist) Team:Hawaii/Project/Part B MaterialsMethodsResults (→Step 4: Device construction)
- 19:14, 29 October 2008 (diff | hist) N File:Prsgsequence.jpg (top)
- 19:14, 29 October 2008 (diff | hist) Team:Hawaii/Project/Part B MaterialsMethodsResults (→Step 4: Device construction)
- 19:14, 29 October 2008 (diff | hist) Team:Hawaii/Project/Part B MaterialsMethodsResults (→Step 4: Device construction)
- 03:52, 29 October 2008 (diff | hist) Team:Hawaii/Project (→Part B: Expression system for Synechocystis sp. PCC 6803)
- 03:35, 29 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-28 (→Project page)
- 03:26, 29 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-28 (→Name of Task)
- 03:25, 29 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-28 (→Antibiotic test)
- 03:05, 29 October 2008 (diff | hist) Team:Hawaii/Project (→Part B: Expression system for Synechocystis sp. PCC 6803)
- 03:04, 29 October 2008 (diff | hist) Team:Hawaii/Project (→Part B: Expression system for Synechocystis sp. PCC 6803)
- 03:03, 29 October 2008 (diff | hist) Team:Hawaii/Project (→Part B: Expression system for Synechocystis sp. PCC 6803)
- 02:40, 29 October 2008 (diff | hist) Team:Hawaii/Project (→Materials, Methods, and Results)
- 02:39, 29 October 2008 (diff | hist) Team:Hawaii/Project/Part B MaterialsMethodsResults (→Step 1: Synthesis and assembly of the nirA promoter and pilA and slr2016 signal sequences)
- 02:38, 29 October 2008 (diff | hist) Team:Hawaii/Project/Part B MaterialsMethodsResults (→Step 1: Synthesis and assembly of the nirA promoter and pilA and slr2016 signal sequences)
- 02:37, 29 October 2008 (diff | hist) Team:Hawaii/Project/Part B MaterialsMethodsResults (→Step 1: Synthesis and assembly of the nirA promoter and pilA and slr2016 signal sequences)
- 02:32, 29 October 2008 (diff | hist) N Team:Hawaii/Project/Part B MaterialsMethodsResults (New page: ==Step 1: Synthesis and assembly of the ''nirA'' promoter and ''pilA'' and ''slr2016'' signal sequences== The ''nir'' promoter and the ''pilA'' and ''slr2016'' secretion signal sequences w...)
- 02:27, 29 October 2008 (diff | hist) Team:Hawaii/Project (→Part B: Expression system for Synechocystis sp. PCC 6803)
- 02:26, 29 October 2008 (diff | hist) Team:Hawaii/Project (→Part B: Expression system for Synechocystis sp. PCC 6803)
- 02:25, 29 October 2008 (diff | hist) Team:Hawaii/Project/Part B (→Materials and Methods) (top)
- 02:23, 29 October 2008 (diff | hist) Team:Hawaii
- 23:53, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Step 1: Synthesis and assembly of the nirA promoter and pilA and slr2016 signal sequences)
- 23:53, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Step 1: Synthesis and assembly of the nirA promoter and pilA and slr2016 signal sequences)
- 23:52, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Step 1: Synthesis and assembly of the nirA promoter and pilA and slr2016 signal sequences)
- 23:52, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Results)
- 23:51, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Step 3: Conversion of pRL1383a into a Biobrick plasmid)
- 23:51, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Step 5: Testing for protein secretion)
- 23:50, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Step 4: Device construction)
- 23:49, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Step 4: Device construction)
- 23:42, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Step 4: Device construction)
- 23:41, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Step 4: Device construction)
- 23:41, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Step 4: Device construction)
- 23:21, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Step 3: Conversion of pRL1383a into a Biobrick plasmid)
- 23:19, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Step 3: Conversion of pRL1383a into a Biobrick plasmid)
- 23:19, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Step 3: Conversion of pRL1383a into a Biobrick plasmid)
- 23:18, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Step 2: Site-directed mutagenesis of GFP (BBa_E0040) into a fusion brick)
- 23:17, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Step 3: Conversion of pRL1383a into a Biobrick plasmid)
- 23:07, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Step 1: Synthesis and assembly of the nirA promoter and pilA and slr2016 signal sequences)
- 23:06, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Step 1: Synthesis and assembly of the nirA promoter and pilA and slr2016 signal sequences)
- 23:06, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Step 1: Synthesis and assembly of the nirA promoter and pilA and slr2016 signal sequences)
- 23:05, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Step 2: Site-directed mutagenesis of GFP (BBa_E0040) into a fusion brick)
- 22:59, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Step 1: Synthesis and assembly of the nirA promoter and pilA and slr2016 signal sequences)
- 22:57, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Step 1: Synthesis and assembly of the nirA promoter and pilA and slr2016 signal sequences)
- 22:49, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Materials and Methods)
- 22:48, 28 October 2008 (diff | hist) Team:Hawaii/Project (→A BioBrick toolkit for cyanobacteria)
- 22:48, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Part B: Expression system for Synechocystis sp. PCC 6803)
- 22:47, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Step 1: Synthesis and assembly of the nirA promoter and pilA and slr2016 signal sequences)
- 22:47, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Expression system for Synechocystis sp. PCC 6803)
- 22:46, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Expression system for Synechocystis sp. PCC 6803=)
- 22:45, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Controls)
- 22:39, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Controls)
- 22:34, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Overall Project)
- 22:33, 28 October 2008 (diff | hist) Team:Hawaii/Project (→=Step 1: Synthesis and assembly of the nirA promoter and pilA and slr2016 signal sequences)
- 22:33, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Expression system for Synechocystis sp. PCC 6803)
- 22:31, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Project Details)
- 22:29, 28 October 2008 (diff | hist) Team:Hawaii/Project (→Results)
- 22:25, 28 October 2008 (diff | hist) Team:Hawaii/Antibiotic test for BB-pRL1383a (→Results)
- 22:24, 28 October 2008 (diff | hist) Team:Hawaii/Antibiotic test for BB-pRL1383a (→Results)
- 22:23, 28 October 2008 (diff | hist) Team:Hawaii/Antibiotic test for BB-pRL1383a (→Verification of plasmid construct)
- 20:39, 28 October 2008 (diff | hist) N Team:Hawaii/Notebook/2008-10-28 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Ran RE digests on gel=== :<strong> Grace</strong> [[Image:102808REdigest.jpg|right|thumb|EtBr stained 4% agarose gel ra...)
- 17:56, 28 October 2008 (diff | hist) Team:Hawaii/Resources (top)
- 17:55, 28 October 2008 (diff | hist) Team:Hawaii
- 17:55, 28 October 2008 (diff | hist) Team:Hawaii/Resources
- 03:20, 28 October 2008 (diff | hist) Team:Hawaii/Event (→Past)
- 03:20, 28 October 2008 (diff | hist) Team:Hawaii/Event (→Soon)
- 03:20, 28 October 2008 (diff | hist) Team:Hawaii/Event (→Future)
- 03:09, 28 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-27 (→Restriction digest)
- 03:08, 28 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-26 (→Plasmid prep)
- 03:08, 28 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-26 (→Plasmid prep)
- 03:08, 28 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-26 (→Plasmid prep)
- 03:08, 28 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-26 (→Plasmid prep)
- 02:09, 28 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-27 (→Plasmid prep of BBpRL1383a)
- 01:44, 28 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-27 (→Triparental conjugation)
- 01:43, 28 October 2008 (diff | hist) N Team:Hawaii/Notebook/2008-10-27 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Plasmid prep of BBpRL1383a=== :<strong> Grace</strong> [[Image:102708plasmid.jpg|right|thumb|250px|EtBr stained 0.8% ag...)
- 22:45, 26 October 2008 (diff | hist) N File:102508plasmiddigest.jpg (top)
- 21:56, 26 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-26 (→Plasmid prep)
- 21:55, 26 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-26 (→Plasmid prep)
- 21:50, 26 October 2008 (diff | hist) N File:102608plasmid.png (top)
- 21:20, 26 October 2008 (diff | hist) N Team:Hawaii/Notebook/2008-10-26 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Plasmid prep=== :<strong> Grace</strong> [[Image:102608plasmid.png|right|thumb|EtBr stained 0.8% agarose gel ran at 95V...)
- 21:19, 26 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-25 (→Verification of plasmid preps)
- 02:44, 26 October 2008 (diff | hist) N Team:Hawaii/Notebook/2008-10-25 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Verification of plasmid preps=== :<strong> Grace</strong> [[Image:102508plasmiddigest.jpg|right|thumb|EtBr stained 4% a...)
- 20:45, 25 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-23 (→Verification of transformants)
- 20:45, 25 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-23 (→Verification of transformants)
- 20:44, 25 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-23 (→Antibiotic test)
- 20:35, 25 October 2008 (diff | hist) N File:102308colonyPCR.png (top)
- 20:11, 25 October 2008 (diff | hist) N File:101608colony.png (top)
- 20:01, 25 October 2008 (diff | hist) Team:Hawaii/Parts (→Parts in process (building))
- 20:00, 25 October 2008 (diff | hist) Team:Hawaii/Parts (→Parts in process (building))
- 22:54, 24 October 2008 (diff | hist) Team:Hawaii/Antibiotic test for BB-pRL1383a (→Results)
- 22:50, 24 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-24 (→Antibiotic test)
- 22:49, 24 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-24 (→Antibiotic test)
- 22:49, 24 October 2008 (diff | hist) N Team:Hawaii/Notebook/2008-10-24 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Prep for sequencing BB1=== :<strong> Grace</strong> :* ExoSAP'd BB1 PCr product and sent to CORE Hawaii for sequencing...)
- 22:35, 24 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-23 (→Large scale plasmid prep)
- 22:33, 24 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-23 (→Large scale plasmid prep)
- 05:07, 24 October 2008 (diff | hist) Team:Hawaii/Antibiotic test for BB-pRL1383a (→Verification of plasmid construct)
- 05:04, 24 October 2008 (diff | hist) Team:Hawaii/Antibiotic test for BB-pRL1383a (→Discussion)
- 05:03, 24 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-23 (→Verification of transformants)
- 05:02, 24 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-23 (→Antibiotic test)
- 23:47, 23 October 2008 (diff | hist) Team:Hawaii/Antibiotic test for BB-pRL1383a (→Discussion)
- 22:13, 23 October 2008 (diff | hist) Team:Hawaii/Antibiotic test for BB-pRL1383a (→Discussion)
- 22:12, 23 October 2008 (diff | hist) Team:Hawaii/Antibiotic test for BB-pRL1383a (→Results)
- 22:11, 23 October 2008 (diff | hist) Team:Hawaii/Antibiotic test for BB-pRL1383a (→Results)
- 22:07, 23 October 2008 (diff | hist) Team:Hawaii/Antibiotic test for BB-pRL1383a (→Results)
- 21:45, 23 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-23 (→Verification of transformants)
- 21:44, 23 October 2008 (diff | hist) N Team:Hawaii/Notebook/2008-10-23 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Antibiotic test=== :<strong> Grace</strong> :* No change from yesterda...)
- 08:56, 23 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-21 (→Insertion of lac-GFP device into BB-pRL1383a)
- 08:56, 23 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-21 (→Insertion of lac-GFP device into BB-pRL1383a)
- 08:54, 23 October 2008 (diff | hist) N File:102108REdigests.png (top)
- 08:51, 23 October 2008 (diff | hist) N File:102108J04430.png (top)
- 08:50, 23 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-21 (→Insertion of lac-GFP device into BB-pRL1383a)
- 08:48, 23 October 2008 (diff | hist) N File:102208J04430.png (top)
- 23:57, 22 October 2008 (diff | hist) Team:Hawaii/Antibiotic test for BB-pRL1383a (→Results)
- 23:56, 22 October 2008 (diff | hist) Team:Hawaii/Antibiotic test for BB-pRL1383a (→Results)
- 23:55, 22 October 2008 (diff | hist) Team:Hawaii/Antibiotic test for BB-pRL1383a (→Transformation)
- 23:44, 22 October 2008 (diff | hist) Team:Hawaii/Antibiotic test for BB-pRL1383a (→Discussion)
- 23:44, 22 October 2008 (diff | hist) Team:Hawaii/Antibiotic test for BB-pRL1383a (→Results)
- 23:44, 22 October 2008 (diff | hist) Team:Hawaii/Antibiotic test for BB-pRL1383a (→Results)
- 23:37, 22 October 2008 (diff | hist) Team:Hawaii/Antibiotic test for BB-pRL1383a (→Results)
- 23:32, 22 October 2008 (diff | hist) Team:Hawaii/Antibiotic test for BB-pRL1383a (→Transformation)
- 23:32, 22 October 2008 (diff | hist) Team:Hawaii/Experiment (→In Progress)
- 22:12, 22 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-22 (→Extracted J04430 from filter paper)
- 20:14, 22 October 2008 (diff | hist) N Team:Hawaii/Notebook/2008-10-22 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Plasmid prep of BB-pRL1383a=== :<strong> Grace</strong> :* ''E. coli'' did not grow. Reinoculated. ===Extracted J0443...)
- 07:42, 22 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-14 (→Construction of secretion device (cont.))
- 07:41, 22 October 2008 (diff | hist) N File:101408REdigest.png (top)
- 07:19, 22 October 2008 (diff | hist) N File:101308colony.jpg (top)
- 07:09, 22 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-11 (→Insertion of secretion device into BBpRL1383a) (top)
- 07:08, 22 October 2008 (diff | hist) File:101108REdigest.jpg (uploaded a new version of "Image:101108REdigest.jpg") (top)
- 07:05, 22 October 2008 (diff | hist) N File:101108REdigest.jpg
- 06:57, 22 October 2008 (diff | hist) N File:100808colony.jpg (top)
- 06:54, 22 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10- 7 (→Construction of secretion device (cont.)) (top)
- 06:42, 22 October 2008 (diff | hist) N File:100708REdigest.png (top)
- 06:40, 22 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10- 7 (→Construction of secretion device (cont.))
- 06:40, 22 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10- 7 (→Construction of secretion device (cont.))
- 03:12, 22 October 2008 (diff | hist) Team:Hawaii/Antibiotic test for BB-pRL1383a (→Transformation)
- 03:11, 22 October 2008 (diff | hist) Team:Hawaii/Antibiotic test for BB-pRL1383a (→Transformation)
- 03:11, 22 October 2008 (diff | hist) N Team:Hawaii/Antibiotic test for BB-pRL1383a (New page: =Antibiotic test and Blue/White screen for BB-pRL1383a= ==Objective== # To verify the successful construction of BB-pRL1383a, a pRL1383a derivative in BioBrick format with blue/white scree...)
- 02:51, 22 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-21 (→Antibiotic test for BB-pRL1383a)
- 23:24, 21 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-21 (→Antibiotic test for BB-pRL1383a)
- 23:23, 21 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-21 (→Antibiotic test for BB-pRL1383a)
- 21:35, 21 October 2008 (diff | hist) Team:Hawaii (Undo revision 59858 by Gracek (Talk))
- 21:34, 21 October 2008 (diff | hist) Team:Hawaii
- 21:33, 21 October 2008 (diff | hist) Team:Hawaii/Project (→Overall Project)
- 21:33, 21 October 2008 (diff | hist) Team:Hawaii/Project (→Overall Project)
- 21:33, 21 October 2008 (diff | hist) Team:Hawaii/Project (→Overall Project)
- 21:32, 21 October 2008 (diff | hist) Team:Hawaii/Project (→Overall Project)
- 21:32, 21 October 2008 (diff | hist) Team:Hawaii/Project (→Overall Project)
- 21:32, 21 October 2008 (diff | hist) Team:Hawaii/Project (→Overall Project)
- 21:31, 21 October 2008 (diff | hist) Team:Hawaii/Project (→Overall Project)
- 21:31, 21 October 2008 (diff | hist) Team:Hawaii/Project (→Overall Project)
- 21:27, 21 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-21 (→Insertion of lac-GFP device into BB-pRL1383a)
- 21:26, 21 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-21 (→Antibiotic test for BB-pRL1383a)
- 18:51, 21 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-21 (→Antibiotic test for BB-pRL1383a)
- 18:51, 21 October 2008 (diff | hist) N Team:Hawaii/Notebook/2008-10-21 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Insertion of lac-GFP device into BB-pRL1383a=== :<strong> Grace</strong> [[Image:102108REdigests.png|right|thumb|EtBr ...)
- 02:09, 21 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-20 (→Made LB+sp+sm plates for antibiotic gradient test on Wednesday)
- 00:01, 21 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-20 (→Large scale plasmid prep of BB-pRL1383a (blue))
- 00:01, 21 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-20 (→Made LB+sp+sm plates for antibiotic gradient test on Wednesday)
- 00:00, 21 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-20 (→PCR of J04430 (lac+rbs+GFP+tt+tt))
- 21:59, 20 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-20 (→Drylab Work)
- 18:41, 20 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-20 (→Wetlab work)
- 18:41, 20 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-18 (→Drylab Work) (top)
- 20:27, 18 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-13 (→Triparental Conjugation) (top)
- 20:26, 18 October 2008 (diff | hist) N File:101308triparents.jpg (top)
- 20:26, 18 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-13 (→Wetlab work)
- 00:57, 18 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-17 (→Restreaked BBpRL1383a construct)
- 19:53, 17 October 2008 (diff | hist) N Team:Hawaii/Notebook/2008-10-17 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Restreaked BBpRL1383a construct=== :<strong> Grace</strong> :* Looked at J33207+pRL1383a (BBpRL1383a) plates. 50 μl...)
- 19:52, 17 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-16 (→Verification of transformants)
- 22:57, 16 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-16 (→Verification of transformants)
- 22:57, 16 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-16 (→Verification of transformants)
- 22:57, 16 October 2008 (diff | hist) N Team:Hawaii/Notebook/2008-10-16 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Verification of transformants=== :<strong> Grace</strong> [[Image101608colony.png|right|thumb|250px|EtBr stained 2% aga...)
- 22:43, 16 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-15 (→Drylab Work)
- 18:23, 15 October 2008 (diff | hist) N Team:Hawaii/Notebook/2008-10-15 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Construction of secretion device (cont.)=== :<strong> Grace</strong> :* Transformed DH5α ::* Used 5 μl of n/p...)
- 18:22, 15 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-14 (→Construction of secretion device (cont.))
- 22:39, 14 October 2008 (diff | hist) N Team:Hawaii/Notebook/2008-10-14 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Construction of secretion device (cont.)=== :<strong> Grace</strong> [[Image:101408REdigest.png|right|thumb|300px|EtBr ...)
- 04:23, 14 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-13 (→Verification of transformants)
- 03:19, 14 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-13 (→Verification of transformants)
- 21:54, 13 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-13 (→Drylab Work)
- 21:52, 13 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-13 (→Verification of transformants)
- 18:18, 13 October 2008 (diff | hist) N Team:Hawaii/Notebook/2008-10-13 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Verification of transformants=== :<strong> Grace</strong> [[Image:101308colony.jpg|right|thumb|250px|EtBr stained 2% ag...)
- 19:52, 12 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-12 (→Verification of Transformants) (top)
- 23:44, 11 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10- 9 (→Triparental conjugtion) (top)
- 23:43, 11 October 2008 (diff | hist) N Team:Hawaii/Notebook/2008-10-12 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Verification of Transformants=== :<strong> Grace</strong> {|class=wikitable border=1 align=center !Construct !Colonies ...)
- 23:08, 11 October 2008 (diff | hist) N Team:Hawaii/Notebook/2008-10-11 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Insertion of secretion device into BBpRL1383a=== :<strong> Grace</strong> [[Image:101108REdigest.jpg|right|thumb|400px|...)
- 03:27, 11 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-10 (→Verification of Transformants) (top)
- 02:31, 11 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10-10 (→verification of Transformants)
- 02:30, 11 October 2008 (diff | hist) N Team:Hawaii/Notebook/2008-10-10 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===verification of Transformants=== :<strong> Grace</strong> [[Image:101008colonypcr.jpg|right|thumb|500px|EtBr stained 2%...)
- 05:22, 10 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10- 9 (→Triparental conjugtion)
- 23:32, 9 October 2008 (diff | hist) N Team:Hawaii/Notebook/2008-10- 9 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Construction of secretion device (cont.)=== :<strong> Grace</strong> [[Image:100908REdigest.jpg|right|thumb|200px|EtBr ...)
- 07:02, 9 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10- 8 (→Construction of secretion device (cont.)) (top)
- 07:01, 9 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10- 8 (→Construction of secretion device (cont.))
- 02:04, 9 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10- 7 (→Discussion)
- 02:04, 9 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10- 8 (→Drylab work)
- 02:04, 9 October 2008 (diff | hist) N Team:Hawaii/Notebook/2008-10- 8 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Triparental conjugation (cont.)=== :<strong> Grace</strong> :* PCR'd BBpRL cultures to ensure that plasmid was retaine...)
- 22:24, 7 October 2008 (diff | hist) N Team:Hawaii/Notebook/2008-10- 7 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Construction of secretion device (cont.)=== :<strong> Grace</strong> :* Ran RE digests on gel :* Extracted bands from ...)
- 00:44, 7 October 2008 (diff | hist) N Team:Hawaii/Notebook/2008-10- 6 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Construction of secretion device (cont.)=== :<strong> Grace</strong> :*PCR'd nir, nir+rbs, rbs+GFP+tt#2, plac+rbs, slr...)
- 22:44, 3 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10- 3 (→Wetlab work) (top)
- 22:32, 3 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10- 3 (→Drylab Work)
- 22:30, 3 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-26 (→Verification of Transformants) (top)
- 22:29, 3 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-26 (→Verification of Transformants)
- 22:29, 3 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-26 (→Verification of Transformants)
- 22:29, 3 October 2008 (diff | hist) N File:092608colonyPCR.jpg (top)
- 22:12, 3 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-22 (→Verification of transformants) (top)
- 22:12, 3 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-22 (→Verification of transformants)
- 22:11, 3 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-22 (→Verification of transformants)
- 22:11, 3 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-22 (→Verification of transformants)
- 22:11, 3 October 2008 (diff | hist) File:092208colonypcr.png (uploaded a new version of "Image:092208colonypcr.png") (top)
- 21:59, 3 October 2008 (diff | hist) N File:092208colonypcr.png
- 21:32, 3 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10- 1 (→Construction of secretion device (cont.)) (top)
- 21:32, 3 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10- 1 (→Construction of secretion device (cont.))
- 21:31, 3 October 2008 (diff | hist) N File:100108REdigest.jpg (top)
- 21:17, 3 October 2008 (diff | hist) File:092508REdigest.jpg (uploaded a new version of "Image:092508REdigest.jpg") (top)
- 21:12, 3 October 2008 (diff | hist) N File:092508REdigest.jpg
- 20:59, 3 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-17 (→Quantification of plasmid preps) (top)
- 20:58, 3 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-20 (→Construction of secretion device (cont.)) (top)
- 20:58, 3 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-20 (→Construction of secretion device (cont.))
- 20:57, 3 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-20 (→Construction of secretion device (cont.))
- 20:57, 3 October 2008 (diff | hist) N File:092008REdigests.png (top)
- 20:40, 3 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10- 3 (→Reran PCR gel for transformant verification)
- 07:43, 3 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10- 3 (→Reran PCR gel for transformant verification)
- 07:43, 3 October 2008 (diff | hist) N Team:Hawaii/Notebook/2008-10- 3 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Reran PCR gel for transformant verification=== :<strong> Grace</strong> [[Image:100308colonyrerun.png|right|thumb|300p...)
- 03:37, 3 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10- 2 (→Verification of transformants)
- 02:34, 3 October 2008 (diff | hist) N Team:Hawaii/Notebook/2008-10- 2 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Verification of transformants=== :<strong> Grace</strong> [[Image:100208colonyPCR.png|right|thumb|300px|EtBr stained 2%...)
- 08:56, 2 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10- 1 (→Construction of secretion device (cont.))
- 00:14, 2 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10- 1 (→Drylab Work)
- 00:14, 2 October 2008 (diff | hist) Team:Hawaii/Notebook/2008-10- 1 (→Construction of secretion device (cont.))
- 23:49, 30 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-10- 1 (→Construction of secretion device (cont.))
- 23:49, 30 September 2008 (diff | hist) N Team:Hawaii/Notebook/2008-10- 1 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Construction of secretion device (cont.)=== :<strong>Grace</strong> [Image:100108REdigest.jpg|right|thumb|200px|EtBr st...)
- 23:43, 30 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-30 (→Construction of secretion device (cont.)) (top)
- 23:42, 30 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-30 (→Construction of secretion device (cont.))
- 23:41, 30 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-30 (→Re-digest from yesterday)
- 18:21, 30 September 2008 (diff | hist) Team:Hawaii/Event (→Soon)
- 18:21, 30 September 2008 (diff | hist) Team:Hawaii/Event
- 08:07, 30 September 2008 (diff | hist) N Team:Hawaii/Notebook/2008-09-30 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Plasmid Prep=== :<strong> Grace</strong> :* Plasmid prep of BBpRL1383a #1, 3, 13, 18 ===Construction of secretion dev...)
- 23:46, 29 September 2008 (diff | hist) N Team:Hawaii/Notebook/2008-09-29 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Prepped DNA for sequencing=== :<strong> Grace</strong> :* plac+rbs :* nrg #1, 4, 7 :* nrsg #2, 8 :* nrp #17 :* rgt #1,...) (top)
- 19:11, 27 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-26 (→Verification of Transformants)
- 19:11, 27 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-26 (→Verification of Transformants)
- 00:00, 27 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-26 (→Verification of Transformants)
- 00:00, 27 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-26 (→Verification of Transformants)
- 23:59, 26 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-26 (→Name of Task)
- 20:16, 26 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-26 (→Verification of Transformants)
- 20:14, 26 September 2008 (diff | hist) N Team:Hawaii/Notebook/2008-09-26 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Verification of Transformants=== :<strong> name of person/people who performed the task</strong> [[Image:092608colonyPC...)
- 23:51, 25 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-25 (→Drylab Work) (top)
- 07:36, 25 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-23 (→PCR: pRL1383a MCS Replacement) (top)
- 07:35, 25 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-23 (→PCR: pRL1383a MCS Replacement)
- 00:25, 25 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-24 (→Prep for sequencing)
- 00:24, 25 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-24 (→Construction of secretion device (cont.))
- 22:27, 24 September 2008 (diff | hist) N Team:Hawaii/Notebook/2008-09-24 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Verified plasmid preps=== :<strong> Grace</strong> :* PCR of plasmid preps from yesterday to verify inserts ::* Ran PC...)
- 03:09, 24 September 2008 (diff | hist) Team:Hawaii/Team (→Students)
- 03:07, 24 September 2008 (diff | hist) N Team:Hawaii/Notebook/2008-09-23 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Plasmid prep=== :<strong> Grace</strong> :* pSB1A3 :* rbs+GFPf+tt #1, 2 :* nir+rbs+GFP #1, 4, 7 :* nir+rbs+slr1+GFPf #...)
- 02:03, 23 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-22 (→Verification of transformants)
- 01:06, 23 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-20 (→Construction of secretion device (cont.))
- 01:06, 23 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-21 (→Drylab Work) (top)
- 01:03, 23 September 2008 (diff | hist) N Team:Hawaii/Notebook/2008-09-22 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Verification of transformants=== :<strong> Grace</strong> [[Image:092208colonypcr.png|right|thumb|300px|EtBr stained 2%...)
- 02:53, 21 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-20 (→Construction of secretion device (cont.))
- 23:01, 20 September 2008 (diff | hist) N Team:Hawaii/Notebook/2008-09-20 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Construction of secretion device (cont.)=== :<strong> Grace</strong> [[Image:092008REdigests.png|right|thumb|300px|EtBr...)
- 22:51, 20 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-19 (→Drylab Work)
- 22:48, 19 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-19 (→Construction of secretion device)
- 21:41, 19 September 2008 (diff | hist) N Team:Hawaii/Notebook/2008-09-19 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Plasmid prep of BB-pRL1383a=== :<strong> Grace</strong> ===Construction of secretion device=== :<strong>Grace</strong> ...)
- 23:47, 18 September 2008 (diff | hist) N Team:Hawaii/Notebook/2008-09-18 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Plasmid prep=== :<strong> Grace</strong> :* Inoculated TB+sp<sub>100</sub> with BB-pRL1383a for plasmid prep ===PCR t...)
- 06:25, 18 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-16 (→Gel Verify constructs)
- 06:25, 18 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-16 (→Drylab Work)
- 00:35, 18 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-17 (→Quantification of plasmid preps)
- 00:34, 18 September 2008 (diff | hist) N File:091708quantification.png (top)
- 20:06, 17 September 2008 (diff | hist) N Team:Hawaii/Notebook/2008-09-17 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Quantification of plasmid preps=== :<strong> Grace</strong> [[Image:091708quantification.png|right|thumb|250px|EtBr sta...)
- 18:16, 16 September 2008 (diff | hist) N Team:Hawaii/Notebook/2008-09-16 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Plasmid prep=== :<strong> Grace</strong> :* 5 ml plasmid prep of: ::* BB-pRL1383a ::* 3A: nir+rbs+slr1 #2, 3 ::* 3A: n...)
- 20:17, 15 September 2008 (diff | hist) Team:Hawaii/Team (→Students)
- 20:16, 15 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-15 (→Drylab Work) (top)
- 08:49, 15 September 2008 (diff | hist) N File:091208colonyPCR.png (top)
- 08:44, 15 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-12 (→Verification of transformants) (top)
- 08:43, 15 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-12 (→Verification of transformants)
- 08:42, 15 September 2008 (diff | hist) File:091208colonyPCR.jpg (uploaded a new version of "Image:091208colonyPCR.jpg") (top)
- 08:13, 15 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-13 (top)
- 23:59, 13 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-12 (→Verification of transformants)
- 23:53, 13 September 2008 (diff | hist) N File:091208colonyPCR.jpg
- 23:44, 13 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-12 (→ON Culture of B_H_R plasmid parts)
- 23:44, 13 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-12 (→Competent Cells)
- 23:43, 13 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-11 (→Plasmid Prep=) (top)
- 23:43, 13 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-11 (→Construction of p+r + s(+gf))
- 23:42, 13 September 2008 (diff | hist) N File:091108REdigests.jpg (top)
- 03:44, 13 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-12 (→Quote of the Day)
- 02:29, 13 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-12 (→Made competent DH5α cells)
- 00:44, 13 September 2008 (diff | hist) N Team:Hawaii/Notebook/2008-09-12 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Verification of transformants=== :<strong> Grace</strong> [[Image:091208colonyPCR.jpg|right|thumb|400px|EtBr stained 2%...)
- 03:45, 12 September 2008 (diff | hist) N Team:Hawaii/Notebook/2008-09-11 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Construction of p+r + s(+g<sub>f</sub>)=== :<strong> Grace</strong> [[Image:091108REdigests.jpg|right|thumb|200px|EtBr ...)
- 03:10, 11 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-10 (→Construction of p+r + s(+gf</sub<)) (top)
- 03:10, 11 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-10 (→Construction of p+r + s(+gf))
- 03:09, 11 September 2008 (diff | hist) N File:090908PCR.jpg (top)
- 02:37, 11 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-10 (→Construction of p+r + s(+gf))
- 02:37, 11 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-10 (→Construction of p+r + s(+gf))
- 02:36, 11 September 2008 (diff | hist) N File:091008REdigests.jpg (top)
- 22:51, 10 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-10 (→Construction of p+r + s(+gf))
- 22:50, 10 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09-10 (→Construction of p+r + s(+gf))
- 22:50, 10 September 2008 (diff | hist) N Team:Hawaii/Notebook/2008-09-10 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Construction of p+r + s(+gf)=== :<strong> Grace</strong> [[Image:091008REdigests.jpg|right|thumb|200px|EtBr stained 2% ...)
- 04:09, 10 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09- 6 (→OriT test) (top)
- 04:09, 10 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09- 6 (→Culturing)
- 04:08, 10 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09- 6 (→Drylab Work)
- 04:08, 10 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09- 9 (→RE digest) (top)
- 03:27, 10 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09- 9 (→PCR)
- 03:25, 10 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09- 9 (→Plasmid prep)
- 18:21, 9 September 2008 (diff | hist) N Team:Hawaii/Notebook/2008-09- 9 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Cryostock=== :<strong> Grace</strong> :* nir+rbs :* rbs+GFP ===Plasmid prep=== :<strong> Grace</strong> :* nir+rbs ...)
- 18:19, 9 September 2008 (diff | hist) N Team:Hawaii/Notebook/2008-09- 8 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Inoculated for plasmid prep and cryostock=== :<strong> Grace</strong> :* nir+rbs :* rbs+GFP :* plac+rbs :* slr1+GFPf :...)
- 02:16, 6 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09- 3 (→Re-replacement of BB-pRL1383a MCS) (top)
- 02:15, 6 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09- 5 (→Verification of transformants from yesterday) (top)
- 02:15, 6 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09- 5 (→Verification of transformants from yesterday)
- 02:14, 6 September 2008 (diff | hist) N File:090508colonypcr.jpg (top)
- 01:42, 6 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09- 4 (→Re-replacement of BB-pRL1383a MCS) (top)
- 01:41, 6 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09- 4 (→Re-replacement of BB-pRL1383a MCS)
- 01:41, 6 September 2008 (diff | hist) N File:090408BBpRL.jpg (top)
- 22:18, 5 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09- 5 (→Sequencing)
- 22:18, 5 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09- 5 (→Sequencing)
- 21:54, 5 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09- 5 (→Construction of Broad-Host-Range Vector Intermediates)
- 07:05, 5 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09- 3 (→Re-replacement of BB-pRL1383a MCS)
- 07:05, 5 September 2008 (diff | hist) N File:090308REdigest.png (top)
- 06:02, 4 September 2008 (diff | hist) N File:090308REdigest.jpg (top)
- 01:22, 4 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09- 4 (→Re-replacement of BB-pRL1383a MCS)
- 01:15, 4 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09- 4 (→3A construction of plac+rbs)
- 01:15, 4 September 2008 (diff | hist) N Team:Hawaii/Notebook/2008-09- 4 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Re-replacement of BB-pRL1383a MCS=== :<strong> Grace</strong> :* Treated inactivated RE digest reaction with SAP :* Ch...)
- 01:09, 4 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09- 3 (→Re-replacement of BB-pRL1383a MCS)
- 21:51, 3 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09- 3 (→Re-replacement of BB-pRL1383a MCS)
- 18:03, 3 September 2008 (diff | hist) N Team:Hawaii/Notebook/2008-09- 3 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Re-replacement of BB-pRL1383a MCS=== :<strong> Grace</strong> [[Image:090308REdigest.jpg|right|thumb|200px|EtBr stained...)
- 03:33, 3 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09- 2 (→Colony PCR)
- 03:33, 3 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-09- 2 (→RE digest)
- 23:39, 2 September 2008 (diff | hist) N Team:Hawaii/Notebook/2008-09- 2 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Plasmid prep=== :<strong>Grace & Krystle</strong> :* BB-pRL1383a :* GFPf + tt colonies #8, 17, and 21 ===Colony PCR===...)
- 06:40, 1 September 2008 (diff | hist) N File:083008REdigests.jpg (top)
- 06:39, 1 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-31 (→Construction of p+r (cont.) and re-replacement of BB-pRL1833a MCS) (top)
- 06:38, 1 September 2008 (diff | hist) N File:083108REdigests.jpg (top)
- 04:22, 1 September 2008 (diff | hist) Team:Hawaii/Protocols/Plasmid prep (→Large Scale Prep) (top)
- 04:14, 1 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-30 (→Wetlab work) (top)
- 04:13, 1 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-30 (→Submitted some parts)
- 04:05, 1 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-31 (→Construction of Broad-Host-Range Plasmid Parts)
- 04:05, 1 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-31 (→Construction of p+r (cont.) and re-replacement of BB-pRL1833a MCS)
- 01:29, 1 September 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-31 (→Construction of p+r (cont.) and re-replacement of BB-pRL1833a MCS)
- 21:27, 31 August 2008 (diff | hist) N Team:Hawaii/Notebook/2008-08-31 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Construction of p+r (cont.) and re-replacement of BB-pRL1833a MCS=== :<strong> Grace</strong> [[Image:083108REdigests.j...)
- 01:32, 31 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-30 (→Construction of p+r and rereplacement of BB-pRL1383a MCS)
- 23:52, 30 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-30 (→Construction of p+r and rereplacement of BB-pRL1383a MCS)
- 21:49, 30 August 2008 (diff | hist) N Team:Hawaii/Notebook/2008-08-30 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Construction of p+r and rereplacement of BB-pRL1383a MCS=== :<strong> Grace</strong> [[Image:083008REdigests.jpg|right|...)
- 22:38, 29 August 2008 (diff | hist) N Team:Hawaii/Notebook/2008-08-29 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===PCR=== :<strong> Grace</strong> :* nir and J33207 from plasmid preps :* Verified products on 1.2% agarose gel ===Plas...)
- 23:42, 28 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-28 (→Checked transformants from yesterday) (top)
- 23:42, 28 August 2008 (diff | hist) N Team:Hawaii/Notebook/2008-08-28 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Checked transformants from yesterday=== :<strong> Grace</strong> {|class=wikitable border=1 !Construct !Colony formi...)
- 08:28, 28 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-27 (→Transformation) (top)
- 08:28, 28 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-27 (→Sequencing)
- 08:27, 28 August 2008 (diff | hist) N File:082708REdigests.jpg (top)
- 23:16, 27 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-27 (→Construction of p+r)
- 23:13, 27 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-27 (→Construction of p+r)
- 23:11, 27 August 2008 (diff | hist) N Team:Hawaii/Notebook/2008-08-27 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Construction of p+r=== :<strong>Grace</strong> :* Ran RE digested products on gel :* Extracted nir, C0012 vector, BB-p...)
- 03:10, 27 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-26 (→Wetlab work)
- 03:09, 27 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-26 (→Wetlab work)
- 03:05, 27 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-25 (→Things we did today) (top)
- 23:01, 26 August 2008 (diff | hist) N Team:Hawaii/Notebook/2008-08-26 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Ran RE digests on gel=== :<strong> Grace</strong> :* Gel did not resolve. Bands were convex, poor separation between b...)
- 06:55, 26 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-25 (→Verification of transformants (cont.))
- 06:54, 26 August 2008 (diff | hist) N File:082508PCR.jpg (top)
- 06:54, 26 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-25 (→Drylab Work)
- 20:11, 24 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-24 (→Verification of transformants)
- 20:10, 24 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-24 (→Verification of transformants)
- 20:10, 24 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-24 (→Verification of plasmid preps (cont.))
- 01:48, 24 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-23 (→Verification of transformants (cont.)) (top)
- 01:47, 24 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-24 (→Drylab Work)
- 01:47, 24 August 2008 (diff | hist) N Team:Hawaii/Notebook/2008-08-24 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Verification of transformants=== :<strong> Grace</strong> {|class=wikitable border=1 align=center !Construct !Colony fo...)
- 01:41, 24 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-23 (→BB-pRL1383a MCS re-replacement)
- 01:41, 24 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-23 (→3A assembly)
- 01:40, 24 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-23 (→Verification of transformants (cont.))
- 13:41, 23 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-22 (→Verification of transformants) (top)
- 13:40, 23 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-22 (→omega interposon)
- 13:40, 23 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-22 (→omega interposon)
- 13:39, 23 August 2008 (diff | hist) N File:082208colonypcr.jpg (top)
- 01:07, 23 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-23 (→3A assembly)
- 01:07, 23 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-23 (→3A assembly)
- 01:07, 23 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-23 (→3A assembly)
- 01:06, 23 August 2008 (diff | hist) N Team:Hawaii/Notebook/2008-08-23 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Verification of transformants (cont.)=== :<strong> Grace</strong> :* Plasmid prep of nir+rbs, rbs+GFP :* Overnight RE ...)
- 00:42, 23 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-22 (→Verification of transformants)
- 22:35, 22 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-22 (→Overnight RE digest)
- 22:32, 22 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-22 (→Verification of transformants)
- 22:32, 22 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-22 (→Verification of transformants)
- 22:32, 22 August 2008 (diff | hist) N Team:Hawaii/Notebook/2008-08-22 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Verification of transformants=== [Image:082208colonypcr.jpg|right|thumb|300px|EtBr stained 2% agarose gel ran at 95v fo...)
- 01:02, 22 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-21 (→BB-pRL1383a MCS re-replacement) (top)
- 00:57, 22 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-21 (→Construction of p+r, r+g, g+t)
- 23:38, 21 August 2008 (diff | hist) N Team:Hawaii/Notebook/2008-08-21 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Construction of p+r, r+g, g+t=== :<strong> Grace</strong> :* Annealed oligonucleotides: ::* plac (I14032) for front li...)
- 08:29, 20 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-19 (→Verification of transformants) (top)
- 08:28, 20 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-19 (→Drylab Work)
- 08:27, 20 August 2008 (diff | hist) N File:081908.jpg (top)
- 08:27, 20 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-19 (→Wetlab work)
- 05:32, 18 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-17 (→Verification of transformants) (top)
- 05:28, 18 August 2008 (diff | hist) N File:081708colonyPCR.jpg (top)
- 00:38, 18 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-17 (→Verification of transformants)
- 00:23, 18 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-17 (→Verification of transformants)
- 06:18, 17 August 2008 (diff | hist) N File:081508REdigest.jpg (top)
- 06:14, 17 August 2008 (diff | hist) N File:081608REdigest.jpg (top)
- 02:03, 17 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-16 (→Plasmid preps) (top)
- 02:03, 17 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-16 (→Reconstruction of BB-pRL1383a (cont.))
- 01:59, 17 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-17 (→Verification of transformants)
- 01:58, 17 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-17 (→Verification of transformants)
- 01:49, 17 August 2008 (diff | hist) Team:Hawaii/Team (→Students)
- 01:47, 17 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-17 (→Verification of transformants)
- 01:46, 17 August 2008 (diff | hist) N Team:Hawaii/Notebook/2008-08-17 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Verification of transformants=== :<strong> Grace</strong> {| class=wikitable border=1 align=center ! DNA ! Total colony...)
- 01:05, 17 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-16 (→Quote of the Day)
- 00:49, 17 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-16 (→Reconstruction of BB-pRL1383a (cont.))
- 00:44, 17 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-16 (→Reconstruction of BB-pRL1383a (cont.))
- 23:23, 16 August 2008 (diff | hist) Team:Hawaii/Experiment
- 23:21, 16 August 2008 (diff | hist) N File:PRL1383agfpsequence.jpg (top)
- 22:31, 16 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-16 (→Reconstruction of BB-pRL1383a (cont.))
- 03:45, 16 August 2008 (diff | hist) N Team:Hawaii/Notebook/2008-08-16 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Reconstruction of BB-pRL1383a (cont.)=== :<strong>Grace</strong> [[Image:081608REdigest.jpg|right|thumb|200px|EtBr stai...)
- 02:52, 16 August 2008 (diff | hist) Team:Hawaii/Team (→Students)
- 02:32, 16 August 2008 (diff | hist) Team:Hawaii/Parts (→Parts in process (building))
- 02:29, 16 August 2008 (diff | hist) Team:Hawaii
- 02:08, 16 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-15 (→Reconstruction of BB-pRL1383a)
- 02:06, 16 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-15 (→Quote of the Day)
- 01:55, 16 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-15 (→Reconstruction of BB-pRL1383a)
- 01:54, 16 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-15 (→Reconstruction of BB-pRL1383a)
- 01:52, 16 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-15 (→Name of Task)
- 01:51, 16 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-15 (→Reconstruction of BB-pRL1383a)
- 01:18, 16 August 2008 (diff | hist) Team:Hawaii/Resources
- 01:17, 16 August 2008 (diff | hist) Team:Hawaii/Resources (→Inventory)
- 01:15, 16 August 2008 (diff | hist) Team:Hawaii
- 01:14, 16 August 2008 (diff | hist) N Team:Hawaii/Resources (New page: {{Team:Hawaii/Header}} ===Inventory=== *[http://packrat.stjohn.hawaii.edu/prestinglab/wiki/iGEM:2008/References References] *[https://2008.igem.org/Team:Hawaii/Protocols Protocols] *[http...)
- 00:59, 16 August 2008 (diff | hist) Team:Hawaii
- 00:15, 16 August 2008 (diff | hist) Team:Hawaii/Team (→Students)
- 00:15, 16 August 2008 (diff | hist) Team:Hawaii/Team (→Advisors)
- 00:14, 16 August 2008 (diff | hist) Team:Hawaii/Team (→Faculty)
- 00:04, 16 August 2008 (diff | hist) Team:Hawaii/Team
- 23:29, 15 August 2008 (diff | hist) Team:Hawaii/Team (→Who we are)
- 23:26, 15 August 2008 (diff | hist) Team:Hawaii/Team (→Where we're from)
- 23:25, 15 August 2008 (diff | hist) Team:Hawaii/Team (→Who we are)
- 23:21, 15 August 2008 (diff | hist) Team:Hawaii/Team (→What we did)
- 23:16, 15 August 2008 (diff | hist) Team:Hawaii/Team (→Who we are)
- 22:46, 15 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-15 (→Wetlab work)
- 22:46, 15 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-15 (→Discussion)
- 22:45, 15 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-15 (→Reconstruction of BB-pRl1383a)
- 22:45, 15 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-15 (→Reconstruction of BB-pRl1383a)
- 20:53, 15 August 2008 (diff | hist) Team:Hawaii/Biobrick conversions (→Sequencing) (top)
- 20:43, 15 August 2008 (diff | hist) Team:Hawaii/Biobrick conversions (→Discussion)
- 20:41, 15 August 2008 (diff | hist) Team:Hawaii/Biobrick conversions (→Attempt #2)
- 20:39, 15 August 2008 (diff | hist) Team:Hawaii/Biobrick conversions (→pRL1383a)
- 20:36, 15 August 2008 (diff | hist) Team:Hawaii/Biobrick conversions (→Results)
- 20:30, 15 August 2008 (diff | hist) Team:Hawaii/Biobrick conversions (→Sequencing)
- 20:29, 15 August 2008 (diff | hist) Team:Hawaii/Biobrick conversions (→Colony PCR to verify transformants)
- 20:29, 15 August 2008 (diff | hist) Team:Hawaii/Biobrick conversions (→Transformed into DB3.1 cells)
- 20:29, 15 August 2008 (diff | hist) Team:Hawaii/Biobrick conversions (→Ligation)
- 20:06, 15 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-15 (→Reconstruction of BB-pRl1383a)
- 19:16, 15 August 2008 (diff | hist) m Team:Hawaii/Notebook/2008-08-14 (→Restriction Digest) (top)
- 19:10, 15 August 2008 (diff | hist) N Team:Hawaii/Notebook/2008-08-15 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Reconstruction of BB-pRl1383a=== :<strong> Grace</strong> :* PCR amplification of J33207 ::* ExoSAP'd PCR product :* R...)
- 17:35, 15 August 2008 (diff | hist) Team:Hawaii/Milestone (top)
- 17:33, 15 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-14 (→Quote of the Day)
- 17:33, 15 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-14 (→Wetlab work)
- 17:21, 15 August 2008 (diff | hist) N File:081408REdigests.jpg (top)
- 09:14, 15 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-14 (→Restriction Digest)
- 09:14, 15 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-14 (→Wetlab work)
- 09:11, 15 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-14 (→Discussion)
- 09:09, 15 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-14 (→Restriction Digest)
- 09:06, 15 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-14 (→Culture)
- 09:06, 15 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-14 (→Colony PCR)
- 09:19, 14 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-13 (→Wetlab work)
- 02:55, 14 August 2008 (diff | hist) N Team:Hawaii/Meeting/2008-08-14 (New page: {{Team:Hawaii/Header}} == Agenda == # Updates # New meeting time(s) for Fall semester == Minutes == Present: <Person A>, <Person B> # == Action Items == * <Person A&...)
- 02:54, 14 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-13 (→Verification of Transformants)
- 02:52, 14 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-13 (→Verification of Transformants)
- 02:49, 14 August 2008 (diff | hist) N File:081308colonyPCR.jpg (top)
- 21:32, 13 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-13 (→Verification of Transformants)
- 19:00, 13 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-13 (→Sequencing)
- 18:14, 13 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-13 (→Wetlab work)
- 18:14, 13 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-13 (→Verification of Transformants)
- 05:41, 13 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-13 (→Verification of Transformants)
- 05:40, 13 August 2008 (diff | hist) N Team:Hawaii/Notebook/2008-08-13 (New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Verification of Transformants=== [[Image:081308colonyPCR.jpg|right|thumb|300px|EtBr stained 2% agarose gel ran at 95V f...)
- 05:34, 13 August 2008 (diff | hist) Team:Hawaii/Protocols/Transformation E coli (→Notes) (top)
- 05:32, 13 August 2008 (diff | hist) Team:Hawaii/Protocols/Plasmid prep (→Miniprep Protocol)
- 05:31, 13 August 2008 (diff | hist) Team:Hawaii/Procols/DNA ligation (→Protocol) (top)
- 05:28, 13 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08- 9 (→Construction of p+r+g, g+t, J33207+t) (top)
- 05:20, 13 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-12 (→Verified transformants) (top)
- 05:20, 13 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-12 (→Test of lab's RE (cont.))
- 05:19, 13 August 2008 (diff | hist) N File:081208colonyPCR.jpg (top)
- 05:12, 13 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-12 (→Verified transformants)
- 05:12, 13 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-12 (→Test of lab's RE (cont.))
- 05:10, 13 August 2008 (diff | hist) N File:081208enzymetest.jpg (top)
- 00:18, 13 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-12 (→3A assembly)
- 00:18, 13 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-12 (→Rear ligation of signal sequences and GFPf)
- 00:17, 13 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-12 (→Name of Task)
- 22:35, 12 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-12 (→3A assembly)
- 22:33, 12 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-12 (→Verified transformants)
- 22:19, 12 August 2008 (diff | hist) Team:Hawaii/Notebook/2008-08-12 (→Test of lab's RE (cont.))
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