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The first challenge of this project was to assemble all the devices we wanted to measure. An overview of these devices can be found here. For the actual construction of all devices the 3A assembly strategy was chosen. A complete protocol of this strategy can be found on OpenWetWare. A schematic representation of this assembly method is depicted in figure 1.

Figure 1. Representation of the 3A assembly method. The black arrow indicates the CcdB 'death' gene. The arrows in other colors indicate different types of antibiotic resistance. Restriction sites are indicated by: E = EcoR1; P = PstI; X = XbaI; S = SpeI