Team:Paris/August 2

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Transformation of 1st August

Results of the transformation

Ligation name Description Antibio Nb colonies observed Fluorescence Comments
puc19 positive control Amp - OK
L100 rbs TetR - ECFP
D110 (BV) - D130 (BI) 		Amp 608 No OK
L101 rbs TetR - GFP tripart
D110 (BV) - D131 (BI) 		Amp > 4000 GFP positive OK
L113 AracpBAD - ecfp
D126 (BV) - D130 (BI) 		Kana 9
L114 AracpBAD - gfp tripart
D126 (BV) - D131 (BI) 		Kana 9
L120 tetR repressible promoter - ECFP
D106 (BV) - D130 (BI) 		Amp 74
L122 RBS-lasI - ecfp
D107 (BV) - D130 (BI) 		Amp 4 No OK
L123 RBS lasI - GFP tripart
D107 (BV) - D131 (BI) 		Amp > 4000 No OK
L126 Strongest RBS (1)- LasR activator (+LVA)
D102 (BV) - D114 (BI) 		Amp 944 No OK
L101

==> Conclusion:Tomorrow we will do a screening PCR on the only Ligation transformant succeed.

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