Team:Paris/July 20

Design of the oligo

for flagellum and mutagenesis of FliA

Culture (in 5ml LB)

only for the minipreps with doubt :

B0034 (3 clones)
R0040 (2 clones)
J23109 (3 clones)
C0179 (3 clones)

Summary of the Biobricks that we owns

B0015  : double terminator
B0030  : RBS.1 (strong)
B0034  : RBS
C0079  : LasR activator
E0030  : YFP
E0040  : GFP
E0422  : CFP
E1010  : mRFP
J23100 : constitutive promotor family member
J23101 : constitutive promotor family member
J23107 : constitutive promotor family member
R0079  : promoter (LasR & PAI regulated)