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Team:Paris/July 14
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| - | + | {{Paris/Calendar_Links|July 13|July 15}} | |
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| + | ==Results of the transformation== | ||
| - | + | * Positive control : pUC19 (1µl; 10pg/ml) --> No transformation | |
| - | + | ||
| - | * Positive control : | + | |
* Biobricks 2007 : --> succeed of the transformation unlike the delay added in the ice | * Biobricks 2007 : --> succeed of the transformation unlike the delay added in the ice | ||
* Biobricks 2008 : --> No transformation | * Biobricks 2008 : --> No transformation | ||
| - | + | Conclusion : Cells are competents, because there is positive transformation | |
| - | + | Hypothesis : problem with the protocol of DNA extraction from the registery 2008 | |
| + | |||
| + | ==Culture of Cells== | ||
| + | * Culture of DH5ɑ in 5ml of LB without Antibiotics, O/N at 37°C | ||
| - | + | ==Preparation of medium and solutions== | |
| - | * | + | * 1L of CCMB80 |
| + | * 500ml of SOB ( 2 x 250ml) | ||
| + | * 500ml of SOC (2 x 250ml) | ||
| + | * 100ml of MgCl<sub>2</sub> (1M) | ||
| + | * 150ml of CaCl<sub>2</sub> (1M) | ||
| + | * 200ml of KOAC (1M) | ||
Latest revision as of 10:08, 16 July 2008
Results of the transformation
Conclusion : Cells are competents, because there is positive transformation
Hypothesis : problem with the protocol of DNA extraction from the registery 2008
Culture of Cells
Preparation of medium and solutions
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