Team:Paris/July 14

From 2008.igem.org

(Difference between revisions)

Latest revision as of 10:08, 16 July 2008

Results of the transformation

Positive control : pUC19 (1µl; 10pg/ml) --> No transformation
Biobricks 2007 : --> succeed of the transformation unlike the delay added in the ice
Biobricks 2008 : --> No transformation

          Conclusion : Cells are competents, because there is positive transformation
          Hypothesis : problem with the protocol of DNA extraction from the registery 2008

Culture of Cells

Culture of DH5ɑ in 5ml of LB without Antibiotics, O/N at 37°C

Preparation of medium and solutions

1L of CCMB80
500ml of SOB ( 2 x 250ml)
500ml of SOC (2 x 250ml)
100ml of MgCl₂ (1M)
150ml of CaCl₂ (1M)
200ml of KOAC (1M)

@@ Line 1: / Line 1: @@
-<center><div style="display: inline; border: thin solid black; background: yellow; color: red;">[[Paris/July 12|&nbsp;< Yesterday]]</div> - <div style="display: inline; border: thin solid black; background: yellow; color: red;">[[Paris/July 14|Tomorrow >&nbsp;]]</div></center>
+{{Paris/Calendar_Links|July 13|July 15}}
-{{Paris/Calendar_Inline}}
+==Results of the transformation==
-[[Results of the transformation :]]
+* Positive control : pUC19 (1µl; 10pg/ml) --> No transformation
-* Positive control : puc19 (1µl; 10pg/ml) --> No transformation
 * Biobricks 2007 : --> succeed of the transformation unlike the delay added in the ice
 * Biobricks 2008 : --> No transformation
@@ Line 11: / Line 9: @@
             Hypothesis : problem with the protocol of DNA extraction from the registery 2008
+==Culture of Cells==
+* Culture of DH5ɑ in 5ml of LB without Antibiotics, O/N at 37°C
-[[Culture of Cells :]]
+==Preparation of medium and solutions==
-* Culture of DH5
+* 1L of CCMB80
+* 500ml of SOB ( 2 x 250ml)
+* 500ml of SOC (2 x 250ml)
+* 100ml of MgCl<sub>2</sub> (1M)
+* 150ml of CaCl<sub>2</sub> (1M)
+* 200ml of KOAC (1M)