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Team:Paris/July 16
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(Difference between revisions)
(New page: == Results of transformations == * Positive controls from biobricks 2007, highest number of bacterias than the first try. It's probably due to the highest quantity of bacteria introduced ...) |
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== Results of transformations == | == Results of transformations == | ||
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* Transformations from biobricks 2008 don't succeed '''again'''. | * Transformations from biobricks 2008 don't succeed '''again'''. | ||
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== Preparation of competent cells == | == Preparation of competent cells == | ||
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* Transfer in an eppendorf tube and spin at 5000rpm during 30" | * Transfer in an eppendorf tube and spin at 5000rpm during 30" | ||
* Remove 800µL of supernatant | * Remove 800µL of supernatant | ||
| - | * Resuspent the pellet in the 150-200µL | + | * Resuspent the pellet in the 150-200µL left |
| + | * Spread on plates | ||
| + | * Incubate O/N at 37°C | ||
Latest revision as of 18:47, 16 July 2008
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Results of transformations
Preparation of competent cells
Transformations Biobricks 2008use of a new protocol --> directly competent cells
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