Team:Paris/July 17

From 2008.igem.org

(Difference between revisions)
(→Results)
(→Results)
Line 127: Line 127:
===Results===
===Results===
 +
===Results===
-
[[Image:080717-1.png]]
 
-
[[Image:080717-2.png]]
+
{|border="1" Paris_PCR_0| Title = J23109 (A)
-
 
+
-
[[Image:080717-3.png]]
+
-
 
+
-
[[Image:080717-4.png]]
+
-
 
+
-
[[Image:080717-5.png]]
+
-
 
+
-
[[Image:080717-6.png]]
+
-
 
+
-
[[Image:080717-7.png]]
+
-
 
+
-
 
+
-
{|Paris_PCR_0| Title = J23109 (A)
+
|PCRs setting
|PCRs setting
|
|
|Quick Load
|Quick Load
|25µL
|25µL
 +
|
|
|
|Expected size
|Expected size
Line 157: Line 145:
|v_oligoF
|v_oligoF
|10µM 1µL
|10µM 1µL
-
|
 
|35pb
|35pb
|-
|-
Line 166: Line 153:
|v_oligoR
|v_oligoR
|10µM 1µL
|10µM 1µL
-
|
 
|Sucess
|Sucess
|-
|-
Line 173: Line 159:
|Water
|Water
|23µL
|23µL
 +
|
|
|
|Yes
|Yes
|-
|-
|1m30'
|1m30'
 +
|
|DNA
|DNA
-
|Cycles= 29
+
|Toothpick of J23109
-
|n_oligoR= 19 VR
+
|
-
|v_oligoR= 10µM 1µL
+
|
-
|water= 23µL
+
|Image band 1-8
-
|DNA= toothpick of J23109
+
|-
-
|Size expected= 35 pb
+
|Number cycles
-
|Success= YES
+
|
-
|Image=
+
|
-
|Band=1-8
+
|
 +
|
 +
|
 +
|link
 +
|-
 +
|29
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|Ladder band 0
|}
|}
 +
 +
[[Image:080717-1.png]]
 +
 +
[[Image:080717-2.png]]
 +
 +
[[Image:080717-3.png]]
 +
 +
[[Image:080717-4.png]]
 +
 +
[[Image:080717-5.png]]
 +
 +
[[Image:080717-6.png]]
 +
 +
[[Image:080717-7.png]]

Revision as of 11:40, 18 July 2008

← Yesterday

↓ Calendar ↑

Tomorrow →



Contents

Results of the transformations (Biobricks 2007)

Legende :

  • few , medium, a lot --> description of the number of CFU (Colony forming unit) produce by the transformation
  • A: Ampicilin, K: Kanamycin


Ref. Book Biobricks 2007 Antibio Results
- positive control A 2.800 CFU
- negative control A 0 CFU
2E_SP 1000 B0034 A few
10F_SP 1001 R0079 A medium
4F_SP 1013 C0079 K few
6H_SP 1001 C0179 A medium
6B_SP 1001 S03154 A 0 CFU
10F_SP 1002 J23101 A a lot
11F_SP 1002 J23109 A a lot
4C_SP 1000 R0040 A 2 CFU
2G_SP 1000 C0040 A 0 CFU
1C_SP 1008 PSB1A3 A few
7G_SP 1013 E1010 K medium
7E_SP 1001 E0040 A 2 CFU
2F_SP 1016 E0030 K a lot


Cloning of the transformations

  • Test 8 clones by Biobricks
  • Incubate O/N at 37°C on proper plates


Screening PCR of the transformations

Use of the same 8 clones of Biobricks that have been tested for cloning


Mix for PCR (x76 tubes)

25µl Quick load 2x 1µl Oligo F 1µl Oligo R 23µl H2O


Protocol

  • 50µl of Mix PCR by tubes
  • 1 clone
  • Programme : 105°C - for 29 cycles : 95°C; 5' - 95°C; 30" - 55°C; 30" - 72°C; 1,5'

Results

Results

PCRs setting Quick Load 25µL Expected size
Annealing n_oligoF 18 VF2 v_oligoF 10µM 1µL 35pb
55°C n_oligoR 19 VR v_oligoR 10µM 1µL Sucess
Time Elongation Water 23µL Yes
1m30' DNA Toothpick of J23109 Image band 1-8
Number cycles link
29 Ladder band 0

080717-1.png

080717-2.png

080717-3.png

080717-4.png

080717-5.png

080717-6.png

080717-7.png

Retrieved from "http://2008.igem.org/Team:Paris/July_17"