Team:Paris/July 20
From 2008.igem.org
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+ | '''Design of the oligo''' | ||
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+ | for flagellum and mutagenesis of FliA | ||
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+ | '''Culture''' (in 5ml LB) | ||
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+ | only for the minipreps with doubt : | ||
+ | B0034 (3 clones) | ||
+ | R0040 (2 clones) | ||
+ | J23109 (3 clones) | ||
+ | C0179 (3 clones) | ||
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+ | '''Summary of the Biobricks that we owns ''' | ||
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+ | B0015 : double terminator | ||
+ | B0030 : RBS.1 (strong) | ||
+ | B0034 : RBS | ||
+ | C0079 : LasR activator | ||
+ | E0030 : YFP | ||
+ | E0040 : GFP | ||
+ | E0422 : CFP | ||
+ | E1010 : mRFP | ||
+ | J23100 : constitutive promotor family member | ||
+ | J23101 : constitutive promotor family member | ||
+ | J23107 : constitutive promotor family member | ||
+ | R0079 : promoter (LasR & PAI regulated) |
Revision as of 15:08, 21 July 2008
for flagellum and mutagenesis of FliA
Culture (in 5ml LB) only for the minipreps with doubt : B0034 (3 clones) R0040 (2 clones) J23109 (3 clones) C0179 (3 clones)
B0015 : double terminator B0030 : RBS.1 (strong) B0034 : RBS C0079 : LasR activator E0030 : YFP E0040 : GFP E0422 : CFP E1010 : mRFP J23100 : constitutive promotor family member J23101 : constitutive promotor family member J23107 : constitutive promotor family member R0079 : promoter (LasR & PAI regulated) |