Team:Paris/July 20

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'''Design of the oligo'''
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for flagellum and mutagenesis of FliA
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'''Culture''' (in 5ml LB)
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only for the minipreps with doubt :
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B0034 (3 clones)
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R0040 (2 clones)
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J23109 (3 clones)
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C0179 (3 clones)
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'''Summary of the Biobricks that we owns '''
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B0015  : double terminator
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B0030  : RBS.1 (strong)
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B0034  : RBS
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C0079  : LasR activator
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E0030  : YFP
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E0040  : GFP
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E0422  : CFP
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E1010  : mRFP
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J23100 : constitutive promotor family member
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J23101 : constitutive promotor family member
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J23107 : constitutive promotor family member
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R0079  : promoter (LasR & PAI regulated)

Revision as of 15:08, 21 July 2008

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Design of the oligo

for flagellum and mutagenesis of FliA


Culture (in 5ml LB)

only for the minipreps with doubt : B0034 (3 clones) R0040 (2 clones) J23109 (3 clones) C0179 (3 clones)


Summary of the Biobricks that we owns

B0015  : double terminator B0030  : RBS.1 (strong) B0034  : RBS C0079  : LasR activator E0030  : YFP E0040  : GFP E0422  : CFP E1010  : mRFP J23100 : constitutive promotor family member J23101 : constitutive promotor family member J23107 : constitutive promotor family member R0079  : promoter (LasR & PAI regulated)