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Team:Paris/July 20
From 2008.igem.org
(Difference between revisions)
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| - | '''Design of the oligo''' | + | =='''Design of the oligo'''== |
for flagellum and mutagenesis of FliA | for flagellum and mutagenesis of FliA | ||
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| - | '''Culture''' (in 5ml LB) | + | =='''Culture''' (in 5ml LB)== |
only for the minipreps with doubt : | only for the minipreps with doubt : | ||
| + | |||
B0034 (3 clones) | B0034 (3 clones) | ||
| + | |||
| + | |||
R0040 (2 clones) | R0040 (2 clones) | ||
| + | |||
J23109 (3 clones) | J23109 (3 clones) | ||
| + | |||
C0179 (3 clones) | C0179 (3 clones) | ||
| - | '''Summary of the Biobricks that we owns ''' | + | =='''Summary of the Biobricks that we owns '''== |
B0015 : double terminator | B0015 : double terminator | ||
| + | |||
B0030 : RBS.1 (strong) | B0030 : RBS.1 (strong) | ||
| + | |||
B0034 : RBS | B0034 : RBS | ||
| + | |||
C0079 : LasR activator | C0079 : LasR activator | ||
| + | |||
E0030 : YFP | E0030 : YFP | ||
| + | |||
E0040 : GFP | E0040 : GFP | ||
| + | |||
E0422 : CFP | E0422 : CFP | ||
| + | |||
E1010 : mRFP | E1010 : mRFP | ||
| + | |||
J23100 : constitutive promotor family member | J23100 : constitutive promotor family member | ||
| + | |||
J23101 : constitutive promotor family member | J23101 : constitutive promotor family member | ||
| + | |||
J23107 : constitutive promotor family member | J23107 : constitutive promotor family member | ||
| + | |||
R0079 : promoter (LasR & PAI regulated) | R0079 : promoter (LasR & PAI regulated) | ||
Revision as of 15:10, 21 July 2008
|
Design of the oligofor flagellum and mutagenesis of FliA
Culture (in 5ml LB)only for the minipreps with doubt : B0034 (3 clones)
J23109 (3 clones) C0179 (3 clones)
Summary of the Biobricks that we ownsB0015 : double terminator B0030 : RBS.1 (strong) B0034 : RBS C0079 : LasR activator E0030 : YFP E0040 : GFP E0422 : CFP E1010 : mRFP J23100 : constitutive promotor family member J23101 : constitutive promotor family member J23107 : constitutive promotor family member R0079 : promoter (LasR & PAI regulated) |
