Team:Paris/July 20
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+ | =='''Design of the oligo'''== | ||
+ | |||
+ | for flagellum and mutagenesis of FliA | ||
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+ | |||
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+ | =='''Culture''' (in 5ml LB)== | ||
+ | |||
+ | only for the minipreps with doubt : | ||
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+ | B0034 (3 clones)<br> | ||
+ | R0040 (2 clones)<br> | ||
+ | J23109 (3 clones)<br> | ||
+ | C0179 (3 clones)<br> | ||
+ | |||
+ | =='''Summary of the Biobricks that we owns '''== | ||
+ | |||
+ | B0015 : double terminator<br> | ||
+ | B0030 : RBS.1 (strong)<br> | ||
+ | B0034 : RBS<br> | ||
+ | C0079 : LasR activator<br> | ||
+ | E0030 : YFP<br> | ||
+ | E0040 : GFP<br> | ||
+ | E0422 : CFP<br> | ||
+ | E1010 : mRFP<br> | ||
+ | J23100 : constitutive promotor family member<br> | ||
+ | J23101 : constitutive promotor family member<br> | ||
+ | J23107 : constitutive promotor family member<br> | ||
+ | R0079 : promoter (LasR & PAI regulated)<br> |
Latest revision as of 15:14, 21 July 2008
Design of the oligofor flagellum and mutagenesis of FliA
Culture (in 5ml LB)only for the minipreps with doubt : B0034 (3 clones) Summary of the Biobricks that we ownsB0015 : double terminator |