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Team:Paris/July 20
From 2008.igem.org
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| - | '''Design of the oligo''' | + | =='''Design of the oligo'''== |
for flagellum and mutagenesis of FliA | for flagellum and mutagenesis of FliA | ||
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| - | '''Culture''' (in 5ml LB) | + | =='''Culture''' (in 5ml LB)== |
only for the minipreps with doubt : | only for the minipreps with doubt : | ||
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| + | B0034 (3 clones)<br> | ||
| + | R0040 (2 clones)<br> | ||
| + | J23109 (3 clones)<br> | ||
| + | C0179 (3 clones)<br> | ||
| - | '''Summary of the Biobricks that we owns ''' | + | =='''Summary of the Biobricks that we owns '''== |
| - | B0015 : double terminator | + | B0015 : double terminator<br> |
| - | B0030 : RBS.1 (strong) | + | B0030 : RBS.1 (strong)<br> |
| - | B0034 : RBS | + | B0034 : RBS<br> |
| - | C0079 : LasR activator | + | C0079 : LasR activator<br> |
| - | E0030 : YFP | + | E0030 : YFP<br> |
| - | E0040 : GFP | + | E0040 : GFP<br> |
| - | E0422 : CFP | + | E0422 : CFP<br> |
| - | E1010 : mRFP | + | E1010 : mRFP<br> |
| - | J23100 : constitutive promotor family member | + | J23100 : constitutive promotor family member<br> |
| - | J23101 : constitutive promotor family member | + | J23101 : constitutive promotor family member<br> |
| - | J23107 : constitutive promotor family member | + | J23107 : constitutive promotor family member<br> |
| - | R0079 : promoter (LasR & PAI regulated) | + | R0079 : promoter (LasR & PAI regulated)<br> |
Latest revision as of 15:14, 21 July 2008
|
Design of the oligofor flagellum and mutagenesis of FliA
Culture (in 5ml LB)only for the minipreps with doubt : B0034 (3 clones) Summary of the Biobricks that we ownsB0015 : double terminator |
