Team:Paris/July 21

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==Quantitative analysis of DNA==
==Quantitative analysis of DNA==
===Electrophoresis===
===Electrophoresis===
 +
Gel : 0.8 % agarose<br>
 +
10 µL Quick-Load 1 kb DNA Ladder<br>
 +
2 µL Bromophenol Blue 5X + 2 µL DNA<br>
 +
 +
====Gel 1====
 +
 +
[[Image:KR000016.jpg‎|right|200px]]
 +
 +
 +
 +
{| border="1"
 +
|align="center"|'''Band'''
 +
|align="center"|'''Biobricks'''
 +
|align="center"|'''Clone'''
 +
|align="center"|'''Expected size'''
 +
|align="center"|'''Measured size'''
 +
|-
 +
|align="left"|1
 +
|align="left"|Ladder 1 kb
 +
|align="center"|&nbsp;
 +
|align="center"|&nbsp;
 +
|align="center"|&nbsp;
 +
|-
 +
|align="left"|2
 +
|align="left"|B0034 : Problem !!!
 +
|align="center"|1
 +
|align="center"|2.091 kb
 +
|align="center"|~ 1.6 kb
 +
|-
 +
|align="left"|3
 +
|align="left"|B0034 : Problem !!!
 +
|align="center"|2
 +
|align="center"|2.091 kb
 +
|align="center"|~ 1.6 kb
 +
|-
 +
|align="left"|4
 +
|align="left"|B0034 : Problem !!!
 +
|align="center"|3
 +
|align="center"|2.091 kb
 +
|align="center"|~ 3 kb
 +
|-
 +
|align="left"|5
 +
|align="left"|R0040 : Problem !!!
 +
|align="center"|1
 +
|align="center"|2.133 kb
 +
|align="center"|~ 1.7 kb
 +
|-
 +
|align="left"|6
 +
|align="left"|R0040 : Problem !!!
 +
|align="center"|2
 +
|align="center"|2.133 kb
 +
|align="center"|~ 1.7 kb
 +
|-
 +
|}
 +
 +
====Gel 2====
 +
 +
[[Image:KR000018.jpg‎|right|200px]]
 +
{| border="1"
 +
|align="center"|'''Band'''
 +
|align="center"|'''Biobricks'''
 +
|align="center"|'''Clone'''
 +
|align="center"|'''Expected size'''
 +
|align="center"|'''Measured size'''
 +
|-
 +
|align="left"|1
 +
|align="left"|Ladder 1 kb
 +
|align="center"|&nbsp;
 +
|align="center"|&nbsp;
 +
|align="center"|&nbsp;
 +
|-
 +
|align="left"|2
 +
|align="left"|J23109 : Problem !!!
 +
|align="center"|1
 +
|align="center"|3.037 kb
 +
|align="center"|~ 1.7 kb
 +
|-
 +
|align="left"|3
 +
|align="left"|J23109 : Problem !!!
 +
|align="center"|2
 +
|align="center"|3.037 kb
 +
|align="center"|~ 5 kb
 +
|-
 +
|align="left"|4
 +
|align="left"|J23109 : Problem !!!
 +
|align="center"|3
 +
|align="center"|3.037 kb
 +
|align="center"|~ 1.7 kb
 +
|-
 +
|align="left"|5
 +
|align="left"|C0179 : Problem !!!
 +
|align="center"|1
 +
|align="center"|2.802 kb
 +
|align="center"|> 10 kb
 +
|-
 +
|align="left"|6
 +
|align="left"|C0179 : Problem !!!
 +
|align="center"|2
 +
|align="center"|2.802 kb
 +
|align="center"|~ 5 kb
 +
|-
 +
|align="left"|7
 +
|align="left"|C0179 : Problem !!!
 +
|align="center"|3
 +
|align="center"|2.802 kb
 +
|align="center"|~ 5 kb
 +
|-
 +
|}
===Measurement of DNA concentration===
===Measurement of DNA concentration===

Latest revision as of 15:29, 21 July 2008

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Contents

Quantitative analysis of DNA

Electrophoresis

Gel : 0.8 % agarose
10 µL Quick-Load 1 kb DNA Ladder
2 µL Bromophenol Blue 5X + 2 µL DNA

Gel 1

KR000016.jpg


Band Biobricks Clone Expected size Measured size
1 Ladder 1 kb      
2 B0034 : Problem !!! 1 2.091 kb ~ 1.6 kb
3 B0034 : Problem !!! 2 2.091 kb ~ 1.6 kb
4 B0034 : Problem !!! 3 2.091 kb ~ 3 kb
5 R0040 : Problem !!! 1 2.133 kb ~ 1.7 kb
6 R0040 : Problem !!! 2 2.133 kb ~ 1.7 kb

Gel 2

KR000018.jpg
Band Biobricks Clone Expected size Measured size
1 Ladder 1 kb      
2 J23109 : Problem !!! 1 3.037 kb ~ 1.7 kb
3 J23109 : Problem !!! 2 3.037 kb ~ 5 kb
4 J23109 : Problem !!! 3 3.037 kb ~ 1.7 kb
5 C0179 : Problem !!! 1 2.802 kb > 10 kb
6 C0179 : Problem !!! 2 2.802 kb ~ 5 kb
7 C0179 : Problem !!! 3 2.802 kb ~ 5 kb

Measurement of DNA concentration
Retrieved from "http://2008.igem.org/Team:Paris/July_21"