Team:Paris/July 23

From 2008.igem.org

(Difference between revisions)

Revision as of 17:03, 23 July 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

Stocks Glycerol

1mL of each culture (with 2 clones) has been added to 1mL of glycerol 40%.
For each clone, two stocks glycerol have been done.
Stored at -20°C.

MiniPreps

The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 22th.

Name	Plasmid
MP100	B0034	Srtongest RBS
MP101	J23101	Stongest constitutive promoter
MP102	J23109	Weak constitutive promoter
MP103	R0079	lasR activator	A
E0030		YFP	K
E0040		GFP	A
E1010		mRFP	K
J23101		constitutive promoter member family	A
J23109		constitutive promoter member family	A
R0040		tetR repressible promoter	A
R0079		promoter (lasr & PAI regulated)	A
pSB1A3		plasmid	A
SO3154		construction intermediate	A
S03879		construction intermediate	A

@@ Line 11: / Line 11: @@
 The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 22th.
-[{J23109 clones 1,2,5<br>
-E0030 clones 1,2,3<br>
+{| border="1"
-pSB1A3 clones 1,2,3<br>
+|align="center"|'''Name'''
-C0179 clones 1,2,7<br>
+|align="center"|'''Plasmid'''
-C0079 clones 1,2,3<br>
-R0079 clones 3,4,6<br>
+|-
-E1010 clones 1,2,3<br>
+|align="center"|MP100
-B0034 clones 1,2,3<br>
+|align="center"|B0034
-J23101 clones 1,2,3<br>
+|align="center"|Srtongest RBS
-E0040 clones 1,2<br>
-R0040 clones 1,2<br>
+|-
+|align="center"|MP101
+|align="center"|J23101
+|align="center"|Stongest constitutive promoter
+|-
+|align="center"|MP102
+|align="center"|J23109
+|align="center"|Weak constitutive promoter
+|-
+|align="center"|MP103
+|align="center"|R0079
+|align="center"|lasR activator
+|align="center"|A
+|-
+|align="center"|E0030
+|align="center"|[[Image:Part_icon_reporter.png]]
+|align="center"|YFP
+|align="center"|K
+|-
+|align="center"|E0040
+|align="center"|[[Image:Part_icon_reporter.png]]
+|align="center"|GFP
+|align="center"|A
+|-
+|align="center"|E1010
+|align="center"|[[Image:Part_icon_reporter.png]]
+|align="center"|mRFP
+|align="center"|K
+|-
+|align="center"|J23101
+|align="center"|[[Image:Part_icon_regulatory.png]]
+|align="center"|constitutive promoter member family
+|align="center"|A
+|-
+|align="center"|J23109
+|align="center"|[[Image:Part_icon_regulatory.png]]
+|align="center"|constitutive promoter member family
+|align="center"|A
+|-
+|align="center"|R0040
+|align="center"|[[Image:Part_icon_regulatory.png]]
+|align="center"|tetR repressible promoter
+|align="center"|A
+|-
+|align="center"|R0079
+|align="center"|[[Image:Part_icon_regulatory.png]]
+|align="center"|promoter (lasr & PAI regulated)
+|align="center"|A
+|-
+|align="center"|pSB1A3
+|align="center"|[[Image:Icon_plasmid.png]]
+|align="center"|plasmid
+|align="center"|A
+|-
+|align="center"|SO3154
+|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
+|align="center"|construction intermediate
+|align="center"|A
+|-
+|align="center"|S03879
+|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
+|align="center"|construction intermediate
+|align="center"|A
+|}