Team:Paris/July 23

From 2008.igem.org

(Difference between revisions)

Revision as of 17:27, 23 July 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

Stocks Glycerol

1mL of each culture (with 2 clones) has been added to 1mL of glycerol 40%.
For each clone, two stocks glycerol have been done.
Stored at -20°C.

MiniPreps

The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 22th.

Name	Plasmid	Description
MP100	B0034	Srtongest RBS (affinity=1)
MP101	J23101	Stongest constitutive promoter
MP102	J23109	Weak constitutive promoter
MP103	R0079	plas
MP104	R0040	pTet
MP105	S03154	RBS-lasI
MP106	S03879	RBS-TetR
MP107	C0079	LasR LVAtag
MP108	C0179	LasR
MP109	E0030	YFP
MP110	E0040	GFP
MP111	E1010	RFP
MP112		OmpR M571
MP113		EnvZ I2815
MP114	C0040	Tetracyclin repressor
MP115	pSB1A3	High copy number plasmid
MP122	E0840	GFP tripart

Cultures

2007 strains have been taken and put in 6mL of LB with the adequat antibiotic. S4 for J23100 (stong constitutive promoter) S5 for B0015 (double terminator) S7 for B0030 (strong RBS : 0,6) S8 for E0422 (ECFP tripart) S13 for I0500 (pBAD) S14 for J23107 (Medium constitutive promoter)

@@ Line 18: / Line 18: @@
 |align="center"|MP100
 |align="center"|B0034
-|align="center"|Srtongest RBS
+|align="center"|Srtongest RBS (affinity=1)
 |-
@@ Line 85: / Line 85: @@
 |align="center"|GFP tripart
 |}
+== Cultures ==
+strains have been taken and put in 6mL of LB with the adequat antibiotic.
+S4 for J23100 (stong constitutive promoter)
+S5 for B0015 (double terminator)
+S7 for B0030 (strong RBS : 0,6)
+S8 for E0422 (ECFP tripart)
+S13 for I0500 (pBAD)
+S14 for J23107 (Medium constitutive promoter)