From 2008.igem.org
(Difference between revisions)
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| | ! rowspan="2"| XbaI | | ! rowspan="2"| XbaI |
| | ! rowspan="2"| PstI | | ! rowspan="2"| PstI |
| - | ! rowspan="2"| BV | + | ! rowspan="2"| |
| | ! rowspan="2"| pb | | ! rowspan="2"| pb |
| | ! rowspan="2"| pb | | ! rowspan="2"| pb |
Revision as of 16:49, 24 July 2008
|
← Yesterday ↓ Calendar ↑Tomorrow →
MiniPreps
- The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 23th.
| Name
| Biobrick
| Description
|
| MP116 (=MP1)
| J23100
|
|
| MP117 (=MP2)
| J23107
|
|
| MP118 (=MP3)
| B0015
|
|
| MP119 (=MP4)
| I0500
|
|
| MP120 (=MP5)
| B0030
|
|
| MP121 (=MP6)
| E0422
|
|
| MP122 (=MP8)
| E0840
|
|
Digestion
Digestion Mix
10µl of Miniprep (clone 1 or 2)
12.5µl of water
2.5µl of Buffer N°2
0.25µl of BSA 100x
1µl of enzyme 1
1µl of enzyme 2
list of digestion
| Name
| Miniprep
| Biobrick
| Enzyme 1
| Enzyme 2
| Nber tube
|
| D100
| MP100
| B0034
| XbaI
| PstI
| 2
|
|
|
|
| EcoRI
| XbaI
| 1
|
|
|
|
| SpeI
| PstI
| 2
|
| D101
| MP101
| J23101
| SpeI
| PstI
| 2
|
| D102
| MP102
| J23109
| SpeI
| PstI
| 2
|
| D103
| MP103
| R0079
| SpeI
| PstI
| 2
|
| D104
| MP104
| R0040
| SpeI
| PstI
| 2
|
| D105
| MP105
| S03154
| SpeI
| PstI
| 1
|
|
|
|
| XbaI
| PstI
| 2
|
|
|
|
| EcoRI
| SpeI
| 1
|
| D106
| MP106
| J23101
| SpeI
| PstI
| 1
|
|
|
|
| XbaI
| PstI
| 2
|
|
|
|
| EcoRI
| SpeI
| 1
|
| D107
| MP107
| C0079
| EcoRI
| SpeI
| 1
|
| D108
| MP108
| C0179
| EcoRI
| SpeI
| 1
|
| D109
| MP109
| E0030
| EcoRI
| SpeI
| 1
|
| D110
| MP110
| E0040
| EcoRI
| SpeI
| 1
|
| D111
| MP111
| E1010
| EcoRI
| SpeI
| 1
|
| D116
| MP116
| J23100
| SpeI
| PstI
| 2
|
| D117
| MP117
| J23107
| SpeI
| PstI
| 2
|
| D118
| MP118
| B0015
| EcoRI
| XbaI
| 2
|
| D119
| MP119
| I0500
| SpeI
| PstI
| 2
|
| D120
| MP120
| B0030
| SpeI
| PstI
| 2
|
|
|
|
| EcoRI
| XbaI
| 1
|
|
|
|
| XbaI
| PstI
| 2
|
| D121
| MP121
| E0422
| XbaI
| PstI
| 3
|
| D122
| MP122
| E0840
| XbaI
| PstI
| 3
|
Results of digestions : Electrophoresis
| Name
| Tube N°
| Enzyme 1
| Enzyme 2
| Observation
| Expected Size Matrix
| Expected Size BioBrick
| Measured Size Matrix
| Measured Size BioBrick
|
| D100
| 1
| XbaI
| PstI
| BI
| 2057 pb
| 34 pb
|
|
|
| 2
|
|
|
| 3
| EcoRI
| XbaI
| FV
| 2076 pb
| 15 pb
|
|
|
| 4
| SpeI
| PstI
| BV
| 2077 pb
| 14 pb
|
|
|
| 5
|
|
|
| D101
| 1
| SpeI
| PstI
| BV
| 2100 pb
| 883 pb
|
|
|
| 2
|
|
|
| D102
| 1
| SpeI
| PstI
| BV
| 2100 pb
| 883 pb
|
|
|
| 2
|
|
|
| D103
| 1
| SpeI
| PstI
| BV
| 2222 pb
| 14 pb
|
|
|
| 2
|
|
|
| D104
| 1
| SpeI
| PstI
| BV
| 2119 pb
| 14 pb
|
|
|
| 2
|
|
|
| D105
| 1
| SpeI
| PstI
| BV
| 2750 pb
| 14 pb
|
|
|
| 2
|
|
|
| D106
| 1
| SpeI
| PstI
| BV
| pb
| 14 pb
|
|
|
| 2
| XbaI
| PstI
|
| pb
| pb
|
|
|
| 3
|
|
|
| 4
| EcoRI
| SpeI
|
| pb
| pb
|
|
|
| D107
| MP107
| C0079
| EcoRI
| SpeI
| 1
|
| D108
| MP108
| C0179
| EcoRI
| SpeI
| 1
|
| D109
| MP109
| E0030
| EcoRI
| SpeI
| 1
|
| D110
| MP110
| E0040
| EcoRI
| SpeI
| 1
|
| D111
| MP111
| E1010
| EcoRI
| SpeI
| 1
|
| D116
| MP116
| J23100
| SpeI
| PstI
| 2
|
| D117
| MP117
| J23107
| SpeI
| PstI
| 2
|
| D118
| MP118
| B0015
| EcoRI
| XbaI
| 2
|
| D119
| MP119
| I0500
| SpeI
| PstI
| 2
|
| D120
| MP120
| B0030
| SpeI
| PstI
| 2
|
|
|
|
| EcoRI
| XbaI
| 1
|
|
|
|
| XbaI
| PstI
| 2
|
| D121
| MP121
| E0422
| XbaI
| PstI
| 3
|
| D122
| MP122
| E0840
| XbaI
| PstI
| 3
|
|
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