Team:Paris/July 24

From 2008.igem.org

(Difference between revisions)
(→Results of digestions : Electrophoresis)
(→Results of digestions : Electrophoresis)
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|align="center"|PstI
|align="center"|PstI
|align="center"|BI
|align="center"|BI
-
|align="center"| 3167 pb
+
|align="center"| 4003 pb
-
|align="center"| 745 pb
+
|align="center"| 778 pb
|align="center"|
|align="center"|
|align="center"|
|align="center"|
|-
|-
-
!align="center" style="background: #ccccff;"|D108
+
! rowspan="2" style="background: #ccccff;"|D108
-
!align="center" style="background: #ccccff;"|C0179
+
! rowspan="2" style="background: #ccccff;"|C0179
|align="center"| 1
|align="center"| 1
-
|align="center"|EcoRI
+
|align="center"| EcoRI
-
|align="center"|SpeI
+
|align="center"| SpeI
-
|align="center"|FI
+
|align="center"| FI
|align="center"| 4402 pb
|align="center"| 4402 pb
|align="center"| 746 pb
|align="center"| 746 pb
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|align="center"|
|align="center"|
|-
|-
-
!align="center" style="background: #ccccff;"|D109
+
|align="center"| 2
-
!align="center" style="background: #ccccff;"|E0030
+
|align="center"| XbaI
 +
|align="center"| PstI
 +
|align="center"| BI
 +
|align="center"| 4403 pb
 +
|align="center"| 745 pb
 +
|align="center"|
 +
|align="center"|
 +
|-
 +
! rowspan="2" style="background: #ccccff;"|D109
 +
! rowspan="2" style="background: #ccccff;"|E0030
|align="center"| 1
|align="center"| 1
|align="center"|EcoRI
|align="center"|EcoRI
|align="center"|SpeI
|align="center"|SpeI
|align="center"|FI
|align="center"|FI
-
|align="center"| 3160 pb
+
|align="center"| 3166 pb
|align="center"| 746 pb
|align="center"| 746 pb
|align="center"|
|align="center"|
|align="center"|
|align="center"|
|-
|-
-
|align="center"|D110
+
|align="center"| 2
-
|align="center"|MP110
+
|align="center"| XbaI
-
|align="center"|E0040
+
|align="center"| PstI
-
|align="center"|EcoRI
+
|align="center"| BI
-
|align="center"|SpeI
+
|align="center"| 3167 pb
-
|align="center"|1
+
|align="center"| 745 pb
 +
|align="center"|
 +
|align="center"|
|-
|-
-
|align="center"|D111
+
! rowspan="2" style="background: #ccccff;"|D110
-
|align="center"|MP111
+
! rowspan="2" style="background: #ccccff;"|E0040
-
|align="center"|E1010
+
|align="center"| 1
-
|align="center"|EcoRI
+
|align="center"| EcoRI
-
|align="center"|SpeI
+
|align="center"| SpeI
-
|align="center"|1
+
|align="center"| FI
 +
|align="center"|  pb
 +
|align="center"|  pb
 +
|align="center"|
 +
|align="center"|
|-
|-
-
|align="center"|D116
+
|align="center"| 2
-
|align="center"|MP116
+
|align="center"| XbaI
-
|align="center"|J23100
+
|align="center"| PstI
-
|align="center"|SpeI
+
|align="center"| BI
-
|align="center"|PstI
+
|align="center"| 2057 pb
-
|align="center"|2
+
|align="center"| 742 pb
 +
|align="center"|
 +
|align="center"|
 +
|-
 +
! rowspan="2" style="background: #ccccff;"|D111
 +
! rowspan="2" style="background: #ccccff;"|E1010
 +
|align="center"| 1
 +
|align="center"| EcoRI
 +
|align="center"| SpeI
 +
|align="center"| FI
 +
|align="center"| 4402 pb
 +
|align="center"| 704 pb
 +
|align="center"|
 +
|align="center"|
 +
|-
 +
|align="center"| 2
 +
|align="center"| XbaI
 +
|align="center"| PstI
 +
|align="center"| BI
 +
|align="center"| 4403 pb
 +
|align="center"| 703 pb
 +
|align="center"|
 +
|align="center"|
 +
|-
 +
! rowspan="2" style="background: #ccccff;"|D116
 +
! rowspan="2" style="background: #ccccff;"|J23107
 +
|align="center"| 1
 +
|align="center"| SpeI
 +
|align="center"| PstI
 +
|align="center"| FI
 +
|align="center"|  pb
 +
|align="center"|  pb
 +
|align="center"|
 +
|align="center"|
|-
|-
|align="center"|D117
|align="center"|D117

Revision as of 18:37, 24 July 2008

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Contents

MiniPreps

  • The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 23th.
Name Biobrick Description
MP116 (=MP1) J23100
MP117 (=MP2) J23107
MP118 (=MP3) B0015
MP119 (=MP4) I0500
MP120 (=MP5) B0030
MP121 (=MP6) E0422
MP122 (=MP8) E0840



Digestion

Digestion Mix

10µl of Miniprep (clone 1 or 2)
12.5µl of water
2.5µl of Buffer N°2
0.25µl of BSA 100x
1µl of enzyme 1
1µl of enzyme 2

list of digestion

Name Miniprep Biobrick Enzyme 1 Enzyme 2 Nber tube
D100 MP100 B0034 XbaI PstI 2
EcoRI XbaI 1
SpeI PstI 2
D101 MP101 J23101 SpeI PstI 2
D102 MP102 J23109 SpeI PstI 2
D103 MP103 R0079 SpeI PstI 2
D104 MP104 R0040 SpeI PstI 2
D105 MP105 S03154 SpeI PstI 1
XbaI PstI 2
EcoRI SpeI 1
D106 MP106 J23101 SpeI PstI 1
XbaI PstI 2
EcoRI SpeI 1
D107 MP107 C0079 EcoRI SpeI 1
D108 MP108 C0179 EcoRI SpeI 1
D109 MP109 E0030 EcoRI SpeI 1
D110 MP110 E0040 EcoRI SpeI 1
D111 MP111 E1010 EcoRI SpeI 1
D116 MP116 J23100 SpeI PstI 2
D117 MP117 J23107 SpeI PstI 2
D118 MP118 B0015 EcoRI XbaI 2
D119 MP119 I0500 SpeI PstI 2
D120 MP120 B0030 SpeI PstI 2
EcoRI XbaI 1
XbaI PstI 2
D121 MP121 E0422 XbaI PstI 3
D122 MP122 E0840 XbaI PstI 3


Results of digestions : Electrophoresis

Name BioBrick Tube N° Enz 1 Enz 2 Obs Exp Size Matrix Exp Size BB Mea Size Matrix Mea Size BB
D100 B0034 1 XbaI PstI BI 2057 pb 34 pb
2
3 EcoRI XbaI FV 2076 pb 15 pb
4 SpeI PstI BV 2077 pb 14 pb
5
D101 J23101 1 SpeI PstI BV 2100 pb 883 pb
2
D102 J23109 1 SpeI PstI BV 2100 pb 883 pb
2
D103 R0079 1 SpeI PstI BV 2222 pb 14 pb
2
D104 R0040 1 SpeI PstI BV 2119 pb 14 pb
2
D105 S03154 1 SpeI PstI BV 2750 pb 14 pb
2 XbaI PstI 2057 pb 707 pb
3
4 EcoRI SpeI 2056 pb 708 pb
D106 S03879 1 SpeI PstI BV 2768 pb 14 pb
2 XbaI PstI 2057 pb 725 pb
3
4 EcoRI SpeI 2756 pb 726 pb
D107 C0079 1 EcoRI SpeI FI 4402 pb 779 pb
2 XbaI PstI BI 4003 pb 778 pb
D108 C0179 1 EcoRI SpeI FI 4402 pb 746 pb
2 XbaI PstI BI 4403 pb 745 pb
D109 E0030 1 EcoRI SpeI FI 3166 pb 746 pb
2 XbaI PstI BI 3167 pb 745 pb
D110 E0040 1 EcoRI SpeI FI pb pb
2 XbaI PstI BI 2057 pb 742 pb
D111 E1010 1 EcoRI SpeI FI 4402 pb 704 pb
2 XbaI PstI BI 4403 pb 703 pb
D116 J23107 1 SpeI PstI FI pb pb
D117 MP117 J23107 SpeI PstI 2
D118 MP118 B0015 EcoRI XbaI 2
D119 MP119 I0500 SpeI PstI 2
D120 MP120 B0030 SpeI PstI 2
EcoRI XbaI 1
XbaI PstI 2
D121 MP121 E0422 XbaI PstI 3
D122 MP122 E0840 XbaI PstI 3
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