Team:Paris/July 25

From 2008.igem.org

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Revision as of 16:44, 25 July 2008

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Contents

MiniPreps

  • The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 24th.
Name Biobrick Description
MP100 B0034
MP116 J23100
MP120 B0030
MP121 E0422
MP122 E0840



Digestion

Digestion Mix

10µl of Miniprep
12.5µl of water
2.5µl of Buffer N°2
0.25µl of BSA 100x
1µl of enzyme 1
1µl of enzyme 2

list of digestion

Name Miniprep Biobrick Enzyme 1 Enzyme 2 Nber tube
D100 MP100 B0034 XbaI PstI 2
EcoRI XbaI 1
SpeI PstI 2
D116 MP116 J23100 SpeI PstI 2
D120 MP120 B0030 SpeI PstI 2
EcoRI XbaI 1
XbaI PstI 2
D121 MP121 E0422 XbaI PstI 1 !
D122 MP122 E0840 XbaI PstI 1 !


Results of digestions : Electrophoresis

Name BioBrick Tube N° Enz 1 Enz 2 Obs Exp Size Matrix Exp Size BB Mea Size Matrix Mea Size BB
D100 B0034 1 XbaI PstI BI 2057 pb 34 pb
2
3 EcoRI XbaI FV 2076 pb 15 pb
4 SpeI PstI BV 2077 pb 14 pb
5
D116 J23100 1 SpeI PstI BV 2100 pb 883 pb
D120 B0030 1 XbaI PstI BI 2057 pb 37 pb
2
3 EcoRI XbaI FV 2079 pb 15 pb
4 SpeI PstI BV 2080 pb 14 pb
5
D121 E0422 1 XbaI PstI FV 2057 pb 939 pb
D122 E0840 1 XbaI PstI FV 2057 pb 900 pb
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