From 2008.igem.org
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| - | == Results of digestions : Electrophoresis == | + | === Results of digestions : Electrophoresis === |
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Revision as of 16:47, 25 July 2008
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← Yesterday ↓ Calendar ↑Tomorrow →
MiniPreps
- The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 24th.
| Name
| Biobrick
| Description
|
| MP100
| B0034
|
|
| MP116
| J23100
|
|
| MP120
| B0030
|
|
| MP121
| E0422
|
|
| MP122
| E0840
|
|
Digestion
Digestion Mix
10µl of Miniprep
12.5µl of water
2.5µl of Buffer N°2
0.25µl of BSA 100x
1µl of enzyme 1
1µl of enzyme 2
list of digestion
| Name
| Miniprep
| Biobrick
| Enzyme 1
| Enzyme 2
| Nber tube
|
| D100
| MP100
| B0034
| XbaI
| PstI
| 2
|
|
|
|
| EcoRI
| XbaI
| 1
|
|
|
|
| SpeI
| PstI
| 2
|
| D116
| MP116
| J23100
| SpeI
| PstI
| 2
|
| D120
| MP120
| B0030
| SpeI
| PstI
| 2
|
|
|
|
| EcoRI
| XbaI
| 1
|
|
|
|
| XbaI
| PstI
| 2
|
| D121
| MP121
| E0422
| XbaI
| PstI
| 1 !
|
| D122
| MP122
| E0840
| XbaI
| PstI
| 1 !
|
Results of digestions : Electrophoresis
| Name
| BioBrick
| Tube N°
| Enz 1
| Enz 2
| Obs
| Exp Size Matrix
| Exp Size BB
| Mea Size Matrix
| Mea Size BB
|
| D100
| B0034
| 1
| XbaI
| PstI
| BI
| 2057 pb
| 34 pb
|
|
|
| 2
|
|
|
| 3
| EcoRI
| XbaI
| FV
| 2076 pb
| 15 pb
|
|
|
| 4
| SpeI
| PstI
| BV
| 2077 pb
| 14 pb
|
|
|
| 5
|
|
|
| D116
| J23100
| 1
| SpeI
| PstI
| BV
| 2100 pb
| 883 pb
|
|
|
| D120
| B0030
| 1
| XbaI
| PstI
| BI
| 2057 pb
| 37 pb
|
|
|
| 2
|
|
|
| 3
| EcoRI
| XbaI
| FV
| 2079 pb
| 15 pb
|
|
|
| 4
| SpeI
| PstI
| BV
| 2080 pb
| 14 pb
|
|
|
| 5
|
|
|
| D121
| E0422
| 1
| XbaI
| PstI
| FV
| 2057 pb
| 939 pb
|
|
|
| D122
| E0840
| 1
| XbaI
| PstI
| FV
| 2057 pb
| 900 pb
|
|
|
|
"
