Team:Paris/July 24

From 2008.igem.org

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Revision as of 11:11, 26 July 2008

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Contents

MiniPreps

  • The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 23th.
Name Biobrick Description
MP116 (=MP1) J23100
MP117 (=MP2) J23107
MP118 (=MP3) B0015
MP119 (=MP4) I0500
MP120 (=MP5) B0030
MP121 (=MP6) E0422
MP122 (=MP8) E0840



Digestion

Digestion Mix

10µl of Miniprep (clone 1 or 2)
12.5µl of water
2.5µl of Buffer N°2
0.25µl of BSA 100x
1µl of enzyme 1
1µl of enzyme 2

list of digestion

Name Miniprep Biobrick Enzyme 1 Enzyme 2 Nber tube
D100 MP100 B0034 XbaI PstI 2
EcoRI XbaI 1
SpeI PstI 2
D101 MP101 J23101 SpeI PstI 2
D102 MP102 J23109 SpeI PstI 2
D103 MP103 R0079 SpeI PstI 2
D104 MP104 R0040 SpeI PstI 2
D105 MP105 S03154 SpeI PstI 1
XbaI PstI 2
EcoRI SpeI 1
D106 MP106 J23101 SpeI PstI 1
XbaI PstI 2
EcoRI SpeI 1
D107 MP107 C0079 EcoRI SpeI 1
D108 MP108 C0179 EcoRI SpeI 1
D109 MP109 E0030 EcoRI SpeI 1
D110 MP110 E0040 EcoRI SpeI 1
D111 MP111 E1010 EcoRI SpeI 1
D116 MP116 J23100 SpeI PstI 2
D117 MP117 J23107 SpeI PstI 2
D118 MP118 B0015 EcoRI XbaI 2
D119 MP119 I0500 SpeI PstI 2
D120 MP120 B0030 SpeI PstI 2
EcoRI XbaI 1
XbaI PstI 2
D121 MP121 E0422 XbaI PstI 3
D122 MP122 E0840 XbaI PstI 3


Results of digestions : Electrophoresis

Name BioBrick Tube N° Enz 1 Enz 2 Obs Exp Size Matrix Exp Size BB Mea Size Matrix Mea Size BB
D100 B0034 1 XbaI PstI BI 2057 pb 34 pb not digested not digested
2
3 EcoRI XbaI FV 2076 pb 15 pb 2100 pb -
4 SpeI PstI BV 2077 pb 14 pb 2000 pb -
5
D101 J23101 1 SpeI PstI BV 2100 pb 883 pb 2000 pb 900 pb
2
D102 J23109 1 SpeI PstI BV 2100 pb 883 pb 2000 pb 900 pb
D103 R0079 1 SpeI PstI BV 2222 pb 14 pb 2500 pb -
2
D104 R0040 1 SpeI PstI BV 2119 pb 14 pb 2500 pb -
2
D105 S03154 1 SpeI PstI BV 2750 pb 14 pb 3000 pb -
2 XbaI PstI 2057 pb 707 pb 2000 pb 700 pb
3
4 EcoRI SpeI 2056 pb 708 pb 2000 pb 700 pb
D106 S03879 1 SpeI PstI BV 2768 pb 14 pb 3000 pb -
2 XbaI PstI 2057 pb 725 pb 2000 pb 700 pb
3
4 EcoRI SpeI 2756 pb 726 pb 2500 pb 700 pb
D107 C0079 1 EcoRI SpeI FI 4402 pb 779 pb 6000 pb 1000 pb
2 XbaI PstI BI 4003 pb 778 pb 5000 pb 100 pb
D108 C0179 1 EcoRI SpeI FI 4402 pb 746 pb 2500 pb 1000 pb
2 XbaI PstI BI 4403 pb 745 pb 2500 pb 1000 pb
D109 E0030 1 EcoRI SpeI FI 3166 pb 746 pb 4000 pb 1000 pb
2 XbaI PstI BI 3167 pb 745 pb 3000 pb 1000 pb
D110 E0040 1 EcoRI SpeI FI 2056 pb 743 pb 2000 pb 800 pb
2 XbaI PstI BI 2057 pb 742 pb 2500 pb 1000 pb
D111 E1010 1 EcoRI SpeI FI 4402 pb 704 pb 4000 pb 600 pb
2 XbaI PstI BI 4403 pb 703 pb 4500 pb 700 pb
D116 J23100 1 SpeI PstI BV 2100 pb 883 pb Not realised
D117 J23107 1 SpeI PstI BV 2100 pb 883 pb 2000 pb 800 pb
D118 B0015 1 EcoRI XbaI FV 3303 pb 15 pb 3000 pb -
D119 I0500 1 SpeI PstI FV 5621 pb 14 pb 6000 - 3000 pb -
D120 B0030 1 XbaI PstI BI 2057 pb 37 pb not digested not digested
2
3 EcoRI XbaI FV 2079 pb 15 pb 1600 pb -
4 SpeI PstI BV 2080 pb 14 pb 3000 - 2000 pb -
5
D121 E0422 1 XbaI PstI FV 2057 pb 939 pb 3000 pb Not digested
D122 E0840 1 XbaI PstI FV 2057 pb 900 pb 2500 pb Not digested

gel1
gel1
gel2
gel2
gel3
gel3
gel4
gel4
gel5
gel5
gel6
gel5
gel7
gel5
gel8
gel5
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