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- | === Results of extraction : Electrophoresis ===
| + | {{Paris/Calendar_Links|July 26|July 28}} |
- | | + | |
- | conditions :
| + | |
- | * 10µl of ladder 1 kb
| + | |
- | * 2µl of extraction added with 2µl of loading Dye 6x
| + | |
- | * migration ~30min at 100W
| + | |
- | * Gel 1, 2, 3, 4, 5 = '''0.8%'''
| + | |
- | * Gel 6 = '''1,5%'''
| + | |
- | | + | |
- | | + | |
- | gel1 [[Image:080728gel_1.jpg| gel1|200px]]
| + | |
- | gel2 [[Image:080728gel_2.jpg| gel2|200px]]
| + | |
- | gel3 [[Image:080728gel_3.jpg| gel3|200px]]
| + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | {| border="1" | + | |
- | |align=center|'''Name'''
| + | |
- | |align=center|'''BioBrick'''
| + | |
- | |align=center|'''Tube N°'''
| + | |
- | |align=center|'''Enz 1'''
| + | |
- | |align=center|'''Enz 2'''
| + | |
- | |align=center|'''Obs'''
| + | |
- | |align=center|'''Exp Size'''
| + | |
- | |align=center|'''Mea Size '''
| + | |
- | |align=center|'''Gel'''
| + | |
- | |align=center|'''Band'''
| + | |
- | |-
| + | |
- | ! rowspan="5" style="background: #ccccff;" | D100
| + | |
- | ! rowspan="5" style="background: #ccccff;" | B0034
| + | |
- | |align=center|1
| + | |
- | ! rowspan="2"| XbaI
| + | |
- | ! rowspan="2"| PstI
| + | |
- | ! rowspan="2"| BI
| + | |
- | ! rowspan="2"| 2057 pb
| + | |
- | ! rowspan="1"|
| + | |
- | ! rowspan="1"|
| + | |
- | ! rowspan="1"|
| + | |
- | |-
| + | |
- | |align=center|2
| + | |
- | ! rowspan="1"|
| + | |
- | ! rowspan="1"|
| + | |
- | |-
| + | |
- | |align=center|3
| + | |
- | |align=center| EcoRI
| + | |
- | |align=center| XbaI
| + | |
- | |align=center| FV
| + | |
- | |align=center| 2076 pb
| + | |
- | |align=center|
| + | |
- | |align=center| 1
| + | |
- | |align=center| 2
| + | |
- | |-
| + | |
- | |align=center|4
| + | |
- | ! rowspan="2"| SpeI
| + | |
- | ! rowspan="2"| PstI
| + | |
- | ! rowspan="2"| BV
| + | |
- | ! rowspan="2"| 2077 pb
| + | |
- | ! rowspan="2"|
| + | |
- | |align=center| 1
| + | |
- | |align=center| 3
| + | |
- | |-
| + | |
- | |align=center|5
| + | |
- | |align=center| 1
| + | |
- | |align=center| 4
| + | |
- | |-
| + | |
- | ! rowspan="2" style="background: #ccccff;" | D101
| + | |
- | ! rowspan="2" style="background: #ccccff;" | J23101
| + | |
- | |align=center|1
| + | |
- | ! rowspan="2"| SpeI
| + | |
- | ! rowspan="2"| PstI
| + | |
- | ! rowspan="2"| BV
| + | |
- | ! rowspan="2"| 2100 pb
| + | |
- | ! rowspan="2"|
| + | |
- | |align=center| 1
| + | |
- | |align=center| 5
| + | |
- | |-
| + | |
- | |align=center|2
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |-
| + | |
- | ! rowspan="2" style="background: #ccccff;" | D102
| + | |
- | ! rowspan="2" style="background: #ccccff;" | J23109
| + | |
- | |align=center|1
| + | |
- | ! rowspan="2"| SpeI
| + | |
- | ! rowspan="2"| PstI
| + | |
- | ! rowspan="2"| BV
| + | |
- | ! rowspan="2"| 2100 pb
| + | |
- | ! rowspan="2"|
| + | |
- | ! rowspan="1"|
| + | |
- | ! rowspan="1"|
| + | |
- | |-
| + | |
- | |align=center|2
| + | |
- | ! rowspan="1"|
| + | |
- | ! rowspan="1"|
| + | |
- | |-
| + | |
- | ! rowspan="2" style="background: #ccccff;" | D103
| + | |
- | ! rowspan="2" style="background: #ccccff;" | R0079
| + | |
- | |align=center|1
| + | |
- | ! rowspan="2"| SpeI
| + | |
- | ! rowspan="2"| PstI
| + | |
- | ! rowspan="2"| BV
| + | |
- | ! rowspan="2"| 2222 pb
| + | |
- | ! rowspan="2"|
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |-
| + | |
- | |align=center|2
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |-
| + | |
- | ! rowspan="2" style="background: #ccccff;" | D104
| + | |
- | ! rowspan="2" style="background: #ccccff;" | R0040
| + | |
- | |align=center|1
| + | |
- | ! rowspan="2"| SpeI
| + | |
- | ! rowspan="2"| PstI
| + | |
- | ! rowspan="2"| BV
| + | |
- | ! rowspan="2"| 2119 pb
| + | |
- | ! rowspan="2"|
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |-
| + | |
- | |align=center|2
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |-
| + | |
- | ! rowspan="4" style="background: #ccccff;" | D105
| + | |
- | ! rowspan="4" style="background: #ccccff;" | S03154
| + | |
- | |align=center|1
| + | |
- | ! rowspan="1"| SpeI
| + | |
- | ! rowspan="1"| PstI
| + | |
- | ! rowspan="1"| BV
| + | |
- | ! rowspan="1"| 2750 pb
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |-
| + | |
- | |align=center|2
| + | |
- | ! rowspan="2"| XbaI
| + | |
- | ! rowspan="2"| PstI
| + | |
- | ! rowspan="2"| BI
| + | |
- | ! rowspan="2"| 707 pb
| + | |
- | ! rowspan="2"|
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |-
| + | |
- | |align=center|3
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |-
| + | |
- | |align=center|4
| + | |
- | |align=center|EcoRI
| + | |
- | |align=center|SpeI
| + | |
- | |align=center|FI
| + | |
- | |align=center| 708 pb
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |-
| + | |
- | ! rowspan="4" style="background: #ccccff;" | D106
| + | |
- | ! rowspan="4" style="background: #ccccff;" | S03879
| + | |
- | |align=center|1
| + | |
- | |align=center|SpeI
| + | |
- | |align=center|PstI
| + | |
- | |align=center|BV
| + | |
- | |align=center| 2768 pb
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |-
| + | |
- | |align=center|2
| + | |
- | ! rowspan="2"| XbaI
| + | |
- | ! rowspan="2"| PstI
| + | |
- | ! rowspan="2"| BI
| + | |
- | ! rowspan="2"| 725 pb
| + | |
- | ! rowspan="2"|
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |-
| + | |
- | |align=center|3
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |-
| + | |
- | |align=center|4
| + | |
- | |align=center|EcoRI
| + | |
- | |align=center|SpeI
| + | |
- | |align=center|FI
| + | |
- | |align=center| 726 pb
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |-
| + | |
- | ! rowspan="2" style="background: #ccccff;" |D107
| + | |
- | ! rowspan="2" style="background: #ccccff;" |C0079
| + | |
- | |align=center| 1
| + | |
- | |align=center|EcoRI
| + | |
- | |align=center|SpeI
| + | |
- | |align=center|FI
| + | |
- | |align=center| 779 pb
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |-
| + | |
- | |align=center| 2
| + | |
- | |align=center|XbaI
| + | |
- | |align=center|PstI
| + | |
- | |align=center|BI
| + | |
- | |align=center| 778 pb
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |-
| + | |
- | ! rowspan="2" style="background: #ccccff;"|D108
| + | |
- | ! rowspan="2" style="background: #ccccff;"|C0179
| + | |
- | |align=center| 1
| + | |
- | |align=center| EcoRI
| + | |
- | |align=center| SpeI
| + | |
- | |align=center| FI
| + | |
- | |align=center| 746 pb
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |-
| + | |
- | |align=center| 2
| + | |
- | |align=center| XbaI
| + | |
- | |align=center| PstI
| + | |
- | |align=center| BI
| + | |
- | |align=center| 745 pb
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |-
| + | |
- | ! rowspan="2" style="background: #ccccff;"|D109
| + | |
- | ! rowspan="2" style="background: #ccccff;"|E0030
| + | |
- | |align=center| 1
| + | |
- | |align=center|EcoRI
| + | |
- | |align=center|SpeI
| + | |
- | |align=center|FI
| + | |
- | |align=center| 746 pb
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |-
| + | |
- | |align=center| 2
| + | |
- | |align=center| XbaI
| + | |
- | |align=center| PstI
| + | |
- | |align=center| BI
| + | |
- | |align=center| 745 pb
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |-
| + | |
- | ! rowspan="2" style="background: #ccccff;"|D110
| + | |
- | ! rowspan="2" style="background: #ccccff;"|E0040
| + | |
- | |align=center| 1
| + | |
- | |align=center| EcoRI
| + | |
- | |align=center| SpeI
| + | |
- | |align=center| FI
| + | |
- | |align=center| 743 pb
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |-
| + | |
- | |align=center| 2
| + | |
- | |align=center| XbaI
| + | |
- | |align=center| PstI
| + | |
- | |align=center| BI
| + | |
- | |align=center| 742 pb
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |-
| + | |
- | ! rowspan="2" style="background: #ccccff;"|D111
| + | |
- | ! rowspan="2" style="background: #ccccff;"|E1010
| + | |
- | |align=center| 1
| + | |
- | |align=center| EcoRI
| + | |
- | |align=center| SpeI
| + | |
- | |align=center| FI
| + | |
- | |align=center| 704 pb
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |-
| + | |
- | |align=center| 2
| + | |
- | |align=center| XbaI
| + | |
- | |align=center| PstI
| + | |
- | |align=center| BI
| + | |
- | |align=center| 703 pb
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |align=center|
| + | |
- | |-
| + | |
- | ! rowspan="2" style="background: #ccccff;"|D116
| + | |
- | ! rowspan="2" style="background: #ccccff;"|J23100
| + | |
- | |align=center|1
| + | |
- | ! rowspan="2"| SpeI
| + | |
- | ! rowspan="2"| PstI
| + | |
- | ! rowspan="2"| BV
| + | |
- | ! rowspan="2"| 2100 pb
| + | |
- | ! rowspan="2"|
| + | |
- | ! rowspan="1"|
| + | |
- | ! rowspan="1"|
| + | |
- | |-
| + | |
- | |align=center|2
| + | |
- | ! rowspan="1"|
| + | |
- | ! rowspan="1"|
| + | |
- | |-
| + | |
- | ! rowspan="2" style="background: #ccccff;"|D117
| + | |
- | ! rowspan="2" style="background: #ccccff;"|J23107
| + | |
- | |align=center|1
| + | |
- | ! rowspan="2"| SpeI
| + | |
- | ! rowspan="2"| PstI
| + | |
- | ! rowspan="2"| BV
| + | |
- | ! rowspan="2"| 2100 pb
| + | |
- | ! rowspan="2"|
| + | |
- | ! rowspan="1"|
| + | |
- | ! rowspan="1"|
| + | |
- | |-
| + | |
- | |align=center|2
| + | |
- | ! rowspan="1"|
| + | |
- | ! rowspan="1"|
| + | |
- | |-
| + | |
- | ! rowspan="2" style="background: #ccccff;"|D118
| + | |
- | ! rowspan="2" style="background: #ccccff;"|B0015
| + | |
- | |align=center|1
| + | |
- | ! rowspan="2"|EcoRI
| + | |
- | ! rowspan="2"|XbaI
| + | |
- | ! rowspan="2"| FV
| + | |
- | ! rowspan="2"| 3303 pb
| + | |
- | ! rowspan="2"|
| + | |
- | ! rowspan="1"|
| + | |
- | ! rowspan="1"|
| + | |
- | |-
| + | |
- | |align=center|2
| + | |
- | ! rowspan="1"|
| + | |
- | ! rowspan="1"|
| + | |
- | |-
| + | |
- | ! rowspan="2" style="background: #ccccff;"|D119
| + | |
- | ! rowspan="2" style="background: #ccccff;"|I0500
| + | |
- | |align=center|1
| + | |
- | ! rowspan="2"|SpeI
| + | |
- | ! rowspan="2"|PstI
| + | |
- | ! rowspan="2"| FV
| + | |
- | ! rowspan="2"| 5621 pb
| + | |
- | ! rowspan="2"|
| + | |
- | ! rowspan="1"|
| + | |
- | ! rowspan="1"|
| + | |
- | |-
| + | |
- | |align=center|2
| + | |
- | ! rowspan="1"|
| + | |
- | ! rowspan="1"|
| + | |
- | |-
| + | |
- | ! rowspan="5" style="background: #ccccff;" | D120
| + | |
- | ! rowspan="5" style="background: #ccccff;" | B0030
| + | |
- | |align=center|1
| + | |
- | ! rowspan="2"| XbaI
| + | |
- | ! rowspan="2"| PstI
| + | |
- | ! rowspan="2"| BI
| + | |
- | ! rowspan="2"| 37 pb
| + | |
- | ! rowspan="2"|
| + | |
- | ! rowspan="1"|
| + | |
- | ! rowspan="1"|
| + | |
- | |-
| + | |
- | |align=center|2
| + | |
- | ! rowspan="1"|
| + | |
- | ! rowspan="1"|
| + | |
- | |-
| + | |
- | |align=center|3
| + | |
- | ! rowspan="1"| EcoRI
| + | |
- | ! rowspan="1"| XbaI
| + | |
- | ! rowspan="1"| FV
| + | |
- | ! rowspan="1"| 2079 pb
| + | |
- | ! rowspan="1"|
| + | |
- | ! rowspan="1"|
| + | |
- | ! rowspan="1"|
| + | |
- | |-
| + | |
- | |align=center|4
| + | |
- | ! rowspan="2"| SpeI
| + | |
- | ! rowspan="2"| PstI
| + | |
- | ! rowspan="2"| BV
| + | |
- | ! rowspan="2"| 2080 pb
| + | |
- | ! rowspan="2"|
| + | |
- | ! rowspan="1"|
| + | |
- | ! rowspan="1"|
| + | |
- | |-
| + | |
- | |align=center|5
| + | |
- | ! rowspan="1"|
| + | |
- | ! rowspan="1"|
| + | |
- | |-
| + | |
- | ! rowspan="3" style="background: #ccccff;"|D121
| + | |
- | ! rowspan="3" style="background: #ccccff;"|E0422
| + | |
- | |align=center|1
| + | |
- | ! rowspan="3"| XbaI
| + | |
- | ! rowspan="3"| PstI
| + | |
- | ! rowspan="3"| BI
| + | |
- | ! rowspan="3"| 939 pb
| + | |
- | ! rowspan="3"|
| + | |
- | ! rowspan="1"|
| + | |
- | ! rowspan="1"|
| + | |
- | |-
| + | |
- | |align=center|2
| + | |
- | ! rowspan="1"|
| + | |
- | ! rowspan="1"|
| + | |
- | |-
| + | |
- | |align=center|3
| + | |
- | ! rowspan="1"|
| + | |
- | ! rowspan="1"|
| + | |
- | |-
| + | |
- | ! rowspan="3" style="background: #ccccff;"|D122
| + | |
- | ! rowspan="3" style="background: #ccccff;"|E0840
| + | |
- | |align=center|1
| + | |
- | ! rowspan="3"| XbaI
| + | |
- | ! rowspan="3"| PstI
| + | |
- | ! rowspan="3"| BI
| + | |
- | ! rowspan="3"| 900 pb
| + | |
- | ! rowspan="1"|
| + | |
- | ! rowspan="1"|
| + | |
- | ! rowspan="1"|
| + | |
- | |-
| + | |
- | |align=center|2
| + | |
- | ! rowspan="1"|
| + | |
- | ! rowspan="1"|
| + | |
- | |-
| + | |
- | |align=center|3
| + | |
- | ! rowspan="1"|
| + | |
- | ! rowspan="1"|
| + | |
- | |} | + | |
- | | + | |
- | | + | |
- | ==> conclusion :
| + | |