Team:Paris/July 30

From 2008.igem.org

(Difference between revisions)
(New page: {{Paris/Calendar_Links|July 29|July 31}} ==Analysis of yesterday DNA digestion== The digested DNA of yesterday was analysed once more time by electrophoresis on a 0.8% agarose gel (about...)
(→Analysis of yesterday DNA digestion)
Line 4: Line 4:
The digested DNA of yesterday was analysed once more time by electrophoresis on a 0.8% agarose gel (about 30 minutes at 100 W). The ladder used was the 1 kb DNA ladder (New England Biolabs). 5 µL of each sample with 1 µL of loading dye were loaded.
The digested DNA of yesterday was analysed once more time by electrophoresis on a 0.8% agarose gel (about 30 minutes at 100 W). The ladder used was the 1 kb DNA ladder (New England Biolabs). 5 µL of each sample with 1 µL of loading dye were loaded.
 +
 +
{|Name|Biobrick|enz 1|enz 2|Obs|Exp size|Mea size|Conc (ng/µL)|Gel|Band
 +
 +
{| border="1"
 +
|align=center|'''Name'''
 +
|align=center|'''BioBrick'''
 +
|align=center|'''Tube N°'''
 +
|align=center|'''Enz 1'''
 +
|align=center|'''Enz 2'''
 +
|align=center|'''Obs'''
 +
|align=center|'''Exp Size'''
 +
|align=center|'''Mea Size '''
 +
|align=center|'''Conc (ng/µl)'''
 +
|align=center|'''Gel'''
 +
|align=center|'''Band'''
 +
|-
 +
! rowspan="1" style="background: #ccccff;" | D101
 +
! rowspan="1" style="background: #ccccff;" | B0034
 +
! rowspan="1"|1
 +
! rowspan="1"| EcoRI
 +
! rowspan="1"| XbaI
 +
! rowspan="1"| FV
 +
! rowspan="1"| 2076 pb
 +
! rowspan="1"|
 +
! rowspan="1"|
 +
! rowspan="1"|
 +
! rowspan="1"| 5
 +
|-
 +
! rowspan="1" style="background: #ccccff;" | D102
 +
! rowspan="1" style="background: #ccccff;" | B0034
 +
! rowspan="1"|1
 +
! rowspan="1"| SpeI
 +
! rowspan="1"| PstI
 +
! rowspan="1"| BV
 +
! rowspan="1"| 2077 pb
 +
! rowspan="1"|
 +
! rowspan="1"|
 +
! rowspan="1"|
 +
! rowspan="1"| 11
 +
|-
 +
! rowspan="1" style="background: #ccccff;" | D105
 +
! rowspan="1" style="background: #ccccff;" | R0079
 +
! rowspan="1"|1
 +
! rowspan="1"| SpeI
 +
! rowspan="1"| PstI
 +
! rowspan="1"| BV
 +
! rowspan="1"| 2222 pb
 +
! rowspan="1"|
 +
! rowspan="1"|
 +
! rowspan="1"|
 +
! rowspan="1"| 13
 +
|-
 +
! rowspan="1" style="background: #ccccff;" | D106
 +
! rowspan="1" style="background: #ccccff;" | R0040
 +
! rowspan="1"|1
 +
! rowspan="1"| SpeI
 +
! rowspan="1"| PstI
 +
! rowspan="1"| BV
 +
! rowspan="1"| 2119 pb
 +
! rowspan="1"|
 +
! rowspan="1"|
 +
! rowspan="1"|
 +
! rowspan="1"| 12
 +
|-
 +
! rowspan="1" style="background: #ccccff;" | D108
 +
! rowspan="1" style="background: #ccccff;" | S03154
 +
! rowspan="1"|1
 +
! rowspan="1"| XbaI
 +
! rowspan="1"| PstI
 +
! rowspan="1"| BI
 +
! rowspan="1"| 707 pb
 +
! rowspan="1"|
 +
! rowspan="1"| 0 (failed)
 +
! rowspan="1"|
 +
! rowspan="1"| 14
 +
|-
 +
! rowspan="1" style="background: #ccccff;" | D111
 +
! rowspan="1" style="background: #ccccff;" | S03879
 +
! rowspan="1"|1
 +
! rowspan="1"| XbaI
 +
! rowspan="1"| PstI
 +
! rowspan="1"| BI
 +
! rowspan="1"| 725 pb
 +
! rowspan="1"|
 +
! rowspan="1"|
 +
! rowspan="1"|
 +
! rowspan="1"| 15
 +
|-
 +
! rowspan="1" style="background: #ccccff;" | D115
 +
! rowspan="1" style="background: #ccccff;" | C0179
 +
! rowspan="1"|2
 +
! rowspan="1"| EcoRI
 +
! rowspan="1"| SpeI
 +
! rowspan="1"| FI
 +
! rowspan="1"| 746 pb
 +
! rowspan="1"|
 +
! rowspan="1"|
 +
! rowspan="1"|
 +
! rowspan="1"| 4 & 5
 +
|-
 +
! rowspan="1" style="background: #ccccff;" | D116
 +
! rowspan="1" style="background: #ccccff;" | C0179
 +
! rowspan="1"|2
 +
! rowspan="1"| XbaI
 +
! rowspan="1"| PstI
 +
! rowspan="1"| BI
 +
! rowspan="1"| 745 pb
 +
! rowspan="1"|
 +
! rowspan="1"|
 +
! rowspan="1"|
 +
! rowspan="1"| 2 & 3
 +
|-
 +
! rowspan="1" style="background: #ccccff;" | D117
 +
! rowspan="1" style="background: #ccccff;" | E0030
 +
! rowspan="1"|1
 +
! rowspan="1"| EcoRI
 +
! rowspan="1"| SpeI
 +
! rowspan="1"| FI
 +
! rowspan="1"| 746 pb
 +
! rowspan="1"|
 +
! rowspan="1"|
 +
! rowspan="1"|
 +
! rowspan="1"| 16
 +
|-
 +
! rowspan="1" style="background: #ccccff;" | D128
 +
! rowspan="1" style="background: #ccccff;" | B0030
 +
! rowspan="1"|2
 +
! rowspan="1"|EcoRI
 +
! rowspan="1"|XbaI
 +
! rowspan="1"|FV
 +
! rowspan="1"| 2079 pb
 +
! rowspan="1"|
 +
! rowspan="1"|
 +
! rowspan="1"|
 +
! rowspan="1"| 6 & 7
 +
|-
 +
! rowspan="1" style="background: #ccccff;" | D129
 +
! rowspan="1" style="background: #ccccff;" | B0030
 +
! rowspan="1"|2
 +
! rowspan="1"|SpeI
 +
! rowspan="1"|PstI
 +
! rowspan="1"|BV
 +
! rowspan="1"| 2080 pb
 +
! rowspan="1"|
 +
! rowspan="1"|
 +
! rowspan="1"|
 +
! rowspan="1"| 8 & 9
 +
|}
 +
 +
Results :
 +
Each of the samples was succesfully digested and purified except for the sample D108. It seems that the QIAprep columms (from the QIAGEN Minipreps kit) can be used instead of the QIAquick columms (for DNA Gel Extraction).

Revision as of 14:32, 31 July 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

Analysis of yesterday DNA digestion

The digested DNA of yesterday was analysed once more time by electrophoresis on a 0.8% agarose gel (about 30 minutes at 100 W). The ladder used was the 1 kb DNA ladder (New England Biolabs). 5 µL of each sample with 1 µL of loading dye were loaded.

Name BioBrick Tube N° Enz 1 Enz 2 Obs Exp Size Mea Size Conc (ng/µl) Gel Band
D101 B0034 1 EcoRI XbaI FV 2076 pb 5
D102 B0034 1 SpeI PstI BV 2077 pb 11
D105 R0079 1 SpeI PstI BV 2222 pb 13
D106 R0040 1 SpeI PstI BV 2119 pb 12
D108 S03154 1 XbaI PstI BI 707 pb 0 (failed) 14
D111 S03879 1 XbaI PstI BI 725 pb 15
D115 C0179 2 EcoRI SpeI FI 746 pb 4 & 5
D116 C0179 2 XbaI PstI BI 745 pb 2 & 3
D117 E0030 1 EcoRI SpeI FI 746 pb 16
D128 B0030 2 EcoRI XbaI FV 2079 pb 6 & 7
D129 B0030 2 SpeI PstI BV 2080 pb 8 & 9

Results : Each of the samples was succesfully digested and purified except for the sample D108. It seems that the QIAprep columms (from the QIAGEN Minipreps kit) can be used instead of the QIAquick columms (for DNA Gel Extraction).

Retrieved from "http://2008.igem.org/Team:Paris/July_30"