Team:Paris/July 31

From 2008.igem.org

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(→Oligonucleotides design)
(→Transformation results)
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==Transformation results==
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=== Results of Ligation transformant ===
{| border="1"  
{| border="1"  
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|align="center"|'''Nb of colonies'''
|align="center"|'''Nb of colonies'''
|align="center"|'''Description'''
|align="center"|'''Description'''
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|align="center"|'''Volume gel extraction'''
 
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|align="center"|L100
|align="center"|L100
|align="center"|0
|align="center"|0
|align="center"|rbs-TetR <-> ECFP (RBS+LVA+Term)
|align="center"|rbs-TetR <-> ECFP (RBS+LVA+Term)
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|align="center"|3+6
 
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|-
|align="center"|L101
|align="center"|L101
|align="center"|(1)
|align="center"|(1)
|align="center"|rbs-TetR <-> GFP tripart+strong rbs  
|align="center"|rbs-TetR <-> GFP tripart+strong rbs  
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|align="center"|3+6
 
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|-
|align="center"|L102
|align="center"|L102
|align="center"|+/- 100
|align="center"|+/- 100
|align="center"|strong RBS (0,6)<-> YFP
|align="center"|strong RBS (0,6)<-> YFP
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|align="center"|5+10
 
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|-
|align="center"|L103
|align="center"|L103
|align="center"|a lot ++  
|align="center"|a lot ++  
|align="center"|strong RBS (0,6)<-> GFP
|align="center"|strong RBS (0,6)<-> GFP
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|align="center"|5+5
 
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|align="center"|L104
|align="center"|L104
|align="center"|a lot ++  
|align="center"|a lot ++  
|align="center"|strong RBS (0,6)<-> LasR activator (+LVA)
|align="center"|strong RBS (0,6)<-> LasR activator (+LVA)
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|align="center"|5+4
 
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|-
|align="center"|L105
|align="center"|L105
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|align="center"|a lot ++  
|align="center"|a lot ++  
|align="center"|strong constitutive promoter <-> GFP tripart
|align="center"|strong constitutive promoter <-> GFP tripart
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|align="center"|5+8
 
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|align="center"|L107
|align="center"|L107
|align="center"|a lot ++  
|align="center"|a lot ++  
|align="center"|strongest constitutive promoter <-> ECFP
|align="center"|strongest constitutive promoter <-> ECFP
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|align="center"|5+8
 
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|align="center"|L108
|align="center"|L108
|align="center"|+/- 100 (2 dishes!?)
|align="center"|+/- 100 (2 dishes!?)
|align="center"|strongest constitutive promoter <-> GFP tripart
|align="center"|strongest constitutive promoter <-> GFP tripart
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|align="center"|5+8
 
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|align="center"|L109
|align="center"|L109
|align="center"|+/- 30 or a lot ++ (2 dishes !?)  
|align="center"|+/- 30 or a lot ++ (2 dishes !?)  
|align="center"|medium constitutive promoter <-> ECFP
|align="center"|medium constitutive promoter <-> ECFP
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|align="center"|4+7
 
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|align="center"|L110
|align="center"|L110
|align="center"|a lot ++
|align="center"|a lot ++
|align="center"|medium constitutive promoter <-> gfp tripart
|align="center"|medium constitutive promoter <-> gfp tripart
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|align="center"|4+7
 
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|align="center"|L111
|align="center"|L111
|align="center"|a lot ++
|align="center"|a lot ++
|align="center"|weak constitutive promoter <-> ECFP
|align="center"|weak constitutive promoter <-> ECFP
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|align="center"|3+5
 
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|align="center"|L112
|align="center"|L112
|align="center"|a lot ++
|align="center"|a lot ++
|align="center"|weak constitutive promoter <-> gfp tripart
|align="center"|weak constitutive promoter <-> gfp tripart
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|align="center"|3+5
 
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|align="center"|L113
|align="center"|L113
|align="center"|1
|align="center"|1
|align="center"|Arac pBAD <-> ECFP
|align="center"|Arac pBAD <-> ECFP
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|align="center"|5+2.5
 
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|align="center"|L114
|align="center"|L114
|align="center"|2
|align="center"|2
|align="center"|Arac pBAD <-> gfp tripart
|align="center"|Arac pBAD <-> gfp tripart
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|align="center"|5+2.5
 
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|align="center"|L115
|align="center"|L115
|align="center"|a lot ++
|align="center"|a lot ++
|align="center"|pLas <-> ECFP
|align="center"|pLas <-> ECFP
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|align="center"|7+11
 
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|align="center"|L116
|align="center"|L116
|align="center"|a lot ++
|align="center"|a lot ++
|align="center"|pLas <-> gfp tripart
|align="center"|pLas <-> gfp tripart
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|align="center"|7+11
 
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|align="center"|L117
|align="center"|L117
|align="center"|+/- 30
|align="center"|+/- 30
|align="center"|Double terminator <-> YFP
|align="center"|Double terminator <-> YFP
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|align="center"|4+3
 
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|align="center"|L118
|align="center"|L118
|align="center"|+/- 30
|align="center"|+/- 30
|align="center"|Double terminator <-> mRFP
|align="center"|Double terminator <-> mRFP
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|align="center"|4+2
 
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|align="center"|L119
|align="center"|L119
|align="center"|+/- 30
|align="center"|+/- 30
|align="center"|Double terminator <-> LasR activator (+LVA)
|align="center"|Double terminator <-> LasR activator (+LVA)
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|align="center"|4+2
 
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|align="center"|L120
|align="center"|L120
|align="center"|a lot ++
|align="center"|a lot ++
|align="center"|tetR repressible promoter <-> ECFP
|align="center"|tetR repressible promoter <-> ECFP
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|align="center"|6+7.5
 
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|align="center"|L121
|align="center"|L121
|align="center"|a lot ++
|align="center"|a lot ++
|align="center"|tetR repressible promoter <-> gfp tripart
|align="center"|tetR repressible promoter <-> gfp tripart
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|align="center"|6+7.5
 
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|align="center"|L122
|align="center"|L122
|align="center"|0
|align="center"|0
|align="center"|B0034rbs-LasI <-> ECFP
|align="center"|B0034rbs-LasI <-> ECFP
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|align="center"|7+3.5
 
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|align="center"|L123
|align="center"|L123
|align="center"|1
|align="center"|1
|align="center"|B0034rbs-LasI <-> gfp tripart
|align="center"|B0034rbs-LasI <-> gfp tripart
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|align="center"|7+3.5
 
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|align="center"|L124
|align="center"|L124
|align="center"|a lot ++
|align="center"|a lot ++
|align="center"|strongest RBS (1) <-> GFP
|align="center"|strongest RBS (1) <-> GFP
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|align="center"|6+5
 
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|align="center"|L125
|align="center"|L125
|align="center"|a lot ++
|align="center"|a lot ++
|align="center"|strongest RBS (1) <-> YFP
|align="center"|strongest RBS (1) <-> YFP
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|align="center"|6+10
 
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|align="center"|L126
|align="center"|L126
|align="center"|no dish found!!!
|align="center"|no dish found!!!
|align="center"|strongest RBS (1) <->  activator (+LVA)
|align="center"|strongest RBS (1) <->  activator (+LVA)
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|align="center"|6+4
 
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|align="center"|L127
|align="center"|L127
|align="center"|no dish found!!!
|align="center"|no dish found!!!
|align="center"|Double terminator <-> GFP
|align="center"|Double terminator <-> GFP
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|align="center"|6+3
 
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|align="center"|'''Controls'''
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|align="center"|'''C1'''
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|align="center"|
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|align="center"|
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|align="center"|
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|align="center"|C1
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|align="center"|0
|align="center"|0
|align="center"|D110
|align="center"|D110
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|align="center"|3
 
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|align="center"|C2
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|align="center"|'''C2'''
|align="center"|5
|align="center"|5
|align="center"|D129
|align="center"|D129
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|align="center"|5
 
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|align="center"|C3
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|align="center"|'''C3'''
|align="center"|+/- 100
|align="center"|+/- 100
|align="center"|D123
|align="center"|D123
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|align="center"|5
 
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|align="center"|C4
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|align="center"|'''C4'''
|align="center"|+/- 20
|align="center"|+/- 20
|align="center"|D103
|align="center"|D103
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|align="center"|5
 
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|align="center"|C5
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|align="center"|'''C5'''
|align="center"|+/- 100
|align="center"|+/- 100
|align="center"|Double terminator <-> GFP
|align="center"|Double terminator <-> GFP
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|align="center"|4
 
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|align="center"|C6
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|align="center"|'''C6'''
|align="center"|+/- 100
|align="center"|+/- 100
|align="center"|Double terminator <-> GFP
|align="center"|Double terminator <-> GFP
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|align="center"|3
 
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|align="center"|C7
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|align="center"|'''C7'''
|align="center"|0
|align="center"|0
|align="center"|Double terminator <-> GFP
|align="center"|Double terminator <-> GFP
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|align="center"|5
 
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|align="center"|C8
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|align="center"|'''C8'''
|align="center"|+/- 20
|align="center"|+/- 20
|align="center"|Double terminator <-> GFP
|align="center"|Double terminator <-> GFP
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|align="center"|7
 
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|align="center"|C9
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|align="center"|'''C9'''
|align="center"|0
|align="center"|0
|align="center"|Double terminator <-> GFP
|align="center"|Double terminator <-> GFP
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|align="center"|4
 
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|-
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|align="center"|C10
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|align="center"|'''C10'''
|align="center"|0
|align="center"|0
|align="center"|Double terminator <-> GFP
|align="center"|Double terminator <-> GFP
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|align="center"|6
 
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|align="center"|C11
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|align="center"|'''C11'''
|align="center"|0
|align="center"|0
|align="center"|Double terminator <-> GFP
|align="center"|Double terminator <-> GFP
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|align="center"|7
 
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|align="center"|C12
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|align="center"|'''C12'''
|align="center"|+/- 10
|align="center"|+/- 10
|align="center"|Double terminator <-> GFP
|align="center"|Double terminator <-> GFP
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|align="center"|6
 
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|align="center"|Positive control
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|align="center"|'''Positive control'''
|align="center"|155 (transformation efficiency:1.5*10^7/ug)
|align="center"|155 (transformation efficiency:1.5*10^7/ug)
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|align="center"|???
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|align="center"|-
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|align="center"|
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|}
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Revision as of 16:58, 4 August 2008

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Results of Ligation transformant

Ligation name Nb of colonies Description
L100 0 rbs-TetR <-> ECFP (RBS+LVA+Term)
L101 (1) rbs-TetR <-> GFP tripart+strong rbs
L102 +/- 100 strong RBS (0,6)<-> YFP
L103 a lot ++ strong RBS (0,6)<-> GFP
L104 a lot ++ strong RBS (0,6)<-> LasR activator (+LVA)
L105 a lot ++ strong constitutive promoter <-> ECFP 5+8
L106 a lot ++ strong constitutive promoter <-> GFP tripart
L107 a lot ++ strongest constitutive promoter <-> ECFP
L108 +/- 100 (2 dishes!?) strongest constitutive promoter <-> GFP tripart
L109 +/- 30 or a lot ++ (2 dishes !?) medium constitutive promoter <-> ECFP
L110 a lot ++ medium constitutive promoter <-> gfp tripart
L111 a lot ++ weak constitutive promoter <-> ECFP
L112 a lot ++ weak constitutive promoter <-> gfp tripart
L113 1 Arac pBAD <-> ECFP
L114 2 Arac pBAD <-> gfp tripart
L115 a lot ++ pLas <-> ECFP
L116 a lot ++ pLas <-> gfp tripart
L117 +/- 30 Double terminator <-> YFP
L118 +/- 30 Double terminator <-> mRFP
L119 +/- 30 Double terminator <-> LasR activator (+LVA)
L120 a lot ++ tetR repressible promoter <-> ECFP
L121 a lot ++ tetR repressible promoter <-> gfp tripart
L122 0 B0034rbs-LasI <-> ECFP
L123 1 B0034rbs-LasI <-> gfp tripart
L124 a lot ++ strongest RBS (1) <-> GFP
L125 a lot ++ strongest RBS (1) <-> YFP
L126 no dish found!!! strongest RBS (1) <-> activator (+LVA)
L127 no dish found!!! Double terminator <-> GFP
C1 0 D110
C2 5 D129
C3 +/- 100 D123
C4 +/- 20 D103
C5 +/- 100 Double terminator <-> GFP
C6 +/- 100 Double terminator <-> GFP
C7 0 Double terminator <-> GFP
C8 +/- 20 Double terminator <-> GFP
C9 0 Double terminator <-> GFP
C10 0 Double terminator <-> GFP
C11 0 Double terminator <-> GFP
C12 +/- 10 Double terminator <-> GFP
Positive control 155 (transformation efficiency:1.5*10^7/ug) -
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