Team:Paris/July 30

From 2008.igem.org

(Difference between revisions)
(→Analysis of yesterday DNA digestion)
(→Analysis of yesterday DNA digestion)
Line 94: Line 94:
! rowspan="1"| SpeI
! rowspan="1"| SpeI
! rowspan="1"| FI
! rowspan="1"| FI
-
! rowspan="1"| 746 pb
+
! rowspan="1"| 746 bp
! rowspan="1"|  
! rowspan="1"|  
! rowspan="1"| 0 (failed)
! rowspan="1"| 0 (failed)
Line 105: Line 105:
! rowspan="1"| PstI
! rowspan="1"| PstI
! rowspan="1"| BI
! rowspan="1"| BI
-
! rowspan="1"| 745 pb
+
! rowspan="1"| 745 bp
-
! rowspan="1"|
+
! rowspan="1"| 700 bp
-
! rowspan="1"|  
+
! rowspan="1"| 2
! rowspan="1"| 2 & 3
! rowspan="1"| 2 & 3
|-
|-
Line 116: Line 116:
! rowspan="1"| SpeI
! rowspan="1"| SpeI
! rowspan="1"| FI
! rowspan="1"| FI
-
! rowspan="1"| 746 pb
+
! rowspan="1"| 746 bp
-
! rowspan="1"|  
+
! rowspan="1"| 600 bp
-
! rowspan="1"|
+
! rowspan="1"| 10
! rowspan="1"| 16
! rowspan="1"| 16
|-
|-
Line 127: Line 127:
! rowspan="1"|XbaI
! rowspan="1"|XbaI
! rowspan="1"|FV
! rowspan="1"|FV
-
! rowspan="1"| 2079 pb
+
! rowspan="1"| 2079 bp
-
! rowspan="1"|  
+
! rowspan="1"| 2000 bp
-
! rowspan="1"|
+
! rowspan="1"| 8
! rowspan="1"| 6 & 7
! rowspan="1"| 6 & 7
|-
|-
Line 138: Line 138:
! rowspan="1"|PstI
! rowspan="1"|PstI
! rowspan="1"|BV
! rowspan="1"|BV
-
! rowspan="1"| 2080 pb
+
! rowspan="1"| 2080 bp
-
! rowspan="1"|  
+
! rowspan="1"| 2000 bp
-
! rowspan="1"|
+
! rowspan="1"| 8
! rowspan="1"| 8 & 9
! rowspan="1"| 8 & 9
|}
|}

Revision as of 16:58, 4 August 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

Analysis of yesterday DNA digestion

The digested DNA of yesterday was analysed one more time by electrophoresis on a 0.8% agarose gel (about 30 minutes at 100 W). The ladder used was the 1 kb DNA ladder (New England Biolabs). 5 µL of each sample with 1 µL of loading dye were loaded.

KR000082.jpg


Name BioBrick Tube N° Enz 1 Enz 2 Obs Exp Size Mea Size Conc (ng/µl) Band
D101 B0034 1 EcoRI XbaI FV 2076 bp 2000 bp 6 10
D102 B0034 1 SpeI PstI BV 2077 bp 2000 bp 8 11
D105 R0079 1 SpeI PstI BV 2222 bp 2000 bp 6 13
D106 R0040 1 SpeI PstI BV 2119 bp 2000 bp 6 12
D108 S03154 1 XbaI PstI BI 707 bp 0 (failed) 14
D111 S03879 1 XbaI PstI BI 725 bp 600 bp 2 15
D115 C0179 2 EcoRI SpeI FI 746 bp 0 (failed) 4 & 5
D116 C0179 2 XbaI PstI BI 745 bp 700 bp 2 2 & 3
D117 E0030 1 EcoRI SpeI FI 746 bp 600 bp 10 16
D128 B0030 2 EcoRI XbaI FV 2079 bp 2000 bp 8 6 & 7
D129 B0030 2 SpeI PstI BV 2080 bp 2000 bp 8 8 & 9

Results : Each of the samples was succesfully digested and purified except for the sample D108. It seems that the QIAprep columms (from the QIAGEN Minipreps kit) can be used instead of the QIAquick columms (for DNA Gel Extraction).

Retrieved from "http://2008.igem.org/Team:Paris/July_30"