Team:Paris/July 30
From 2008.igem.org
(→Results of transformations) |
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|align="center"|'''Description''' | |align="center"|'''Description''' | ||
|align="center"|'''Antibio''' | |align="center"|'''Antibio''' | ||
- | |align="center"|'''Nb colonies | + | |align="center"|'''Nb colonies''' |
|align="center"|'''Comments''' | |align="center"|'''Comments''' | ||
|- | |- | ||
|align="center"|L100 | |align="center"|L100 | ||
- | |align="center"| | + | |align="center"|rbs TetR - ECFP<br>D110 (BV) - D130 (BI) |
- | rbs TetR - ECFP | + | |
- | <br>D110 (BV) - D130 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|0 | |align="center"|0 | ||
- | |align="center"|After another night nothing was osberved -> To do again | + | |align="center"|After another night nothing was osberved <br> -> To do again |
|- | |- | ||
|align="center"|L101 | |align="center"|L101 | ||
- | |align="center"| | + | |align="center"|rbs TetR - GFP tripart<br>D110 (BV) - D131 (BI) |
- | rbs TetR - GFP tripart | + | |
- | <br>D110 (BV) - D131 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|1 | |align="center"|1 | ||
- | |align="center"|After another night nothing was observed -> To do again | + | |align="center"|After another night nothing was observed <br> -> To do again |
|- | |- | ||
|align="center"|L102 | |align="center"|L102 | ||
- | |align="center"| | + | |align="center"|Strong rbs - YFP<br>D129 (BV) - D118 (BI) |
- | Strong rbs - YFP | + | |
- | <br>D129 (BV) - D118 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|+/- 100 | |align="center"|+/- 100 | ||
Line 39: | Line 33: | ||
|- | |- | ||
|align="center"|L103 | |align="center"|L103 | ||
- | |align="center"| | + | |align="center"|Strong rbs - GFP<br>D129 (BV) - D122 (BI) |
- | Strong rbs - GFP | + | |
- | <br>D129 (BV) - D122 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|a lot | |align="center"|a lot | ||
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|- | |- | ||
|align="center"|L104 | |align="center"|L104 | ||
- | |align="center"| | + | |align="center"|Strong rbs - lasR activator<br>D129 (BV) - D114 (BI) |
- | Strong rbs - lasR activator | + | |
- | <br>D129 (BV) - D114 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|a lot | |align="center"|a lot | ||
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|- | |- | ||
|align="center"|L105 | |align="center"|L105 | ||
- | |align="center"| | + | |align="center"|Strong promoter - ECFP<br>D123 (BV) - D130 (BI) |
- | Strong promoter - ECFP | + | |
- | <br>D123 (BV) - D130 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|a lot | |align="center"|a lot | ||
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|- | |- | ||
|align="center"|L106 | |align="center"|L106 | ||
- | |align="center"| | + | |align="center"|Strong promoter - gfp Tripart<br>D123 (BV) - D131 (BI) |
- | Strong promoter - gfp Tripart | + | |
- | <br>D123 (BV) - D131 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|a lot | |align="center"|a lot | ||
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|- | |- | ||
|align="center"|L107 | |align="center"|L107 | ||
- | |align="center"| | + | |align="center"|Strongest promoter - ECFP<br>D103 (BV) - D131 (BI) |
- | Strongest promoter - ECFP | + | |
- | <br>D103 (BV) - D131 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|a lot | |align="center"|a lot | ||
|align="center"| | |align="center"| | ||
|- | |- | ||
- | |align="center"|L108 n°2 (the right one) | + | |align="center"|L108 n°2 <br>(the right one) |
- | |align="center"| | + | |align="center"|Strong promoter - gfp Tripart<br>D103 (BV) - D130 (BI) |
- | Strong promoter - gfp Tripart | + | |
- | <br>D103 (BV) - D130 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|+/- 100 | |align="center"|+/- 100 | ||
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|- | |- | ||
|align="center"|L109 n°1 | |align="center"|L109 n°1 | ||
- | |align="center"| | + | |align="center"|Strong promoter - ecfp<br>D124 (BV) - D130 (BI) |
- | Strong promoter - ecfp | + | |
- | <br>D124 (BV) - D130 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|+/- 30 | |align="center"|+/- 30 | ||
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|- | |- | ||
|align="center"|L109 n°2 | |align="center"|L109 n°2 | ||
- | |align="center"| | + | |align="center"|Strong promoter - ecfp <br>D124 (BV) - D130 (BI) |
- | Strong promoter - ecfp | + | |
- | <br>D124 (BV) - D130 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|a lot | |align="center"|a lot | ||
Line 103: | Line 81: | ||
|- | |- | ||
|align="center"|L110 | |align="center"|L110 | ||
- | |align="center"| | + | |align="center"|Medium promoter - gfp Tripart<br>D124 (BV) - D131 (BI) |
- | Medium promoter - gfp Tripart | + | |
- | <br>D124 (BV) - D131 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|a lot | |align="center"|a lot | ||
Line 111: | Line 87: | ||
|- | |- | ||
|align="center"|L111 | |align="center"|L111 | ||
- | |align="center"| | + | |align="center"|Weak promoter - ECFP<br>D104 (BV) - D130 (BI) |
- | Weak promoter - ECFP | + | |
- | <br>D104 (BV) - D130 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|a lot | |align="center"|a lot | ||
Line 119: | Line 93: | ||
|- | |- | ||
|align="center"|L112 | |align="center"|L112 | ||
- | |align="center"| | + | |align="center"|Weak promoter - gfp<br>D104 (BV) - D131 (BI) |
- | Weak promoter - gfp | + | |
- | <br>D104 (BV) - D131 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|a lot | |align="center"|a lot | ||
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|- | |- | ||
|align="center"|L113 | |align="center"|L113 | ||
- | |align="center"| | + | |align="center"|AracpBAD - ecfp<br>D126 (BV) - D130 (BI) |
- | AracpBAD - ecfp | + | |
- | <br>D126 (BV) - D130 (BI) | + | |
|align="center"|Kan | |align="center"|Kan | ||
|align="center"|1 | |align="center"|1 | ||
- | |align="center"|After another night nothing was observed -> To do again | + | |align="center"|After another night nothing was observed <br> -> To do again |
|- | |- | ||
|align="center"|L114 | |align="center"|L114 | ||
- | |align="center"| | + | |align="center"|AracpBAD - gfp tripart<br>D126 (BV) - D131 (BI) |
- | AracpBAD - gfp tripart | + | |
- | <br>D126 (BV) - D131 (BI) | + | |
|align="center"|Kan | |align="center"|Kan | ||
|align="center"|2 | |align="center"|2 | ||
- | |align="center"|After another night nothing was observed -> To do again | + | |align="center"|After another night nothing was observed <br>-> To do again |
|- | |- | ||
|align="center"|L115 | |align="center"|L115 | ||
- | |align="center"| | + | |align="center"|pLas - ECFP<br>D105 (BV) - D130 (BI) |
- | pLas - ECFP | + | |
- | <br>D105 (BV) - D130 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|a lot | |align="center"|a lot | ||
Line 151: | Line 117: | ||
|- | |- | ||
|align="center"|L116 | |align="center"|L116 | ||
- | |align="center"| | + | |align="center"|pLas - gfp Tripart<br>D105 (BV) - D131 (BI) |
- | pLas - gfp Tripart | + | |
- | <br>D105 (BV) - D131 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|a lot | |align="center"|a lot | ||
Line 159: | Line 123: | ||
|- | |- | ||
|align="center"|L117 | |align="center"|L117 | ||
- | |align="center"| | + | |align="center"|yfp - Double Terminator<br>D117 (FI) - D125 (FV) |
- | yfp - Double Terminator | + | |
- | <br>D117 (FI) - D125 (FV) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|+/- 30 | |align="center"|+/- 30 | ||
Line 167: | Line 129: | ||
|- | |- | ||
|align="center"|L118 | |align="center"|L118 | ||
- | |align="center"| | + | |align="center"|rfp - Double Terminator<br>D121 (FI) - D125 (FV) |
- | rfp - Double Terminator | + | |
- | <br>D121 (FI) - D125 (FV) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|+/- 30 | |align="center"|+/- 30 | ||
Line 175: | Line 135: | ||
|- | |- | ||
|align="center"|L119 | |align="center"|L119 | ||
- | |align="center"| | + | |align="center"|lasR activator + LVA - Double Terminator<br>D113 (FI) - D125 (FV) |
- | lasR activator + LVA - Double Terminator | + | |
- | <br>D113 (FI) - D125 (FV) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|+/- 30 | |align="center"|+/- 30 | ||
Line 183: | Line 141: | ||
|- | |- | ||
|align="center"|L120 | |align="center"|L120 | ||
- | |align="center"| | + | |align="center"|tetR repressible - ECFP<br>D106 (BV) - D130 (BI) |
- | tetR repressible - ECFP | + | |
- | <br>D106 (BV) - D130 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|a lot | |align="center"|a lot | ||
Line 191: | Line 147: | ||
|- | |- | ||
|align="center"|L121 | |align="center"|L121 | ||
- | |align="center"| | + | |align="center"|Strong promoter - gfp tripart<br>D106 (BV) - D130 (BI) |
- | Strong promoter - gfp tripart | + | |
- | <br>D106 (BV) - D130 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|a lot | |align="center"|a lot | ||
Line 199: | Line 153: | ||
|- | |- | ||
|align="center"|L122 | |align="center"|L122 | ||
- | |align="center"| | + | |align="center"|RBS-lasI - ecfp<br>D107 (BV) - D130 (BI) |
- | RBS-lasI - ecfp | + | |
- | <br>D107 (BV) - D130 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|0 | |align="center"|0 | ||
- | |align="center"|After another night nothing was observed -> To do again | + | |align="center"|After another night nothing was observed <br>-> To do again |
|- | |- | ||
|align="center"|L123 | |align="center"|L123 | ||
- | |align="center"| | + | |align="center"|RBS lasI - ecfp<br>D107 (BV) - D131 (BI) |
- | RBS lasI - ecfp | + | |
- | <br>D107 (BV) - D131 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|1 | |align="center"|1 | ||
- | |align="center"|After another night nothing was observed -> To do again | + | |align="center"|After another night nothing was observed <br>-> To do again |
|- | |- | ||
|align="center"|L124 | |align="center"|L124 | ||
- | |align="center"| | + | |align="center"|Strongest RBS - rfp<br>D102 (BV) - D122 (BI) |
- | Strongest RBS - rfp | + | |
- | <br>D102 (BV) - D122 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|a lot | |align="center"|a lot | ||
Line 223: | Line 171: | ||
|- | |- | ||
|align="center"|L125 | |align="center"|L125 | ||
- | |align="center"| | + | |align="center"|Strongest RBS - yfp<br>D102 (BV) - D118 (BI) |
- | Strongest RBS - yfp | + | |
- | <br>D102 (BV) - D118 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|a lot | |align="center"|a lot | ||
Line 231: | Line 177: | ||
|- | |- | ||
|align="center"|L126 | |align="center"|L126 | ||
- | |align="center"| | + | |align="center"|Strongest RBS (1)- LacR activator (+LVA)<br>D102 (BV) - D118 (BI) |
- | Strongest RBS (1)- LacR activator (+LVA) | + | |
- | <br>D102 (BV) - D118 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|no dish found!!! | |align="center"|no dish found!!! | ||
Line 239: | Line 183: | ||
|- | |- | ||
|align="center"|L127 | |align="center"|L127 | ||
- | |align="center"| | + | |align="center"|gfp (1)- Double terminator<br>D119 (FI) - D125 (FV) |
- | gfp (1)- Double terminator | + | |
- | <br>D119 (FI) - D125 (FV) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|no dish found!!! | |align="center"|no dish found!!! | ||
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puc19 | puc19 | ||
|align="center"|Amp | |align="center"|Amp | ||
- | |align="center"|155 (transformation efficiency:1.5*10^7/ug) | + | |align="center"|155 (transformation <br> efficiency:1.5*10^7/ug) |
|align="center"|- | |align="center"|- | ||
|} | |} |
Revision as of 16:59, 5 August 2008
Results of transformations
Analysis of yesterday DNA digestionThe digested DNA of yesterday was analysed one more time by electrophoresis on a 0.8% agarose gel (about 30 minutes at 100 W). The ladder used was the 1 kb DNA ladder (New England Biolabs). 5 µL of each sample with 1 µL of loading dye were loaded.
Results : Each of the samples was succesfully digested and purified except for the sample D108. It seems that the QIAprep columms (from the QIAGEN Minipreps kit) can be used instead of the QIAquick columms (for DNA Gel Extraction).
PCR Screening of Ligation TransformantsUse of 8 clones of Ligation transformants for screening PCR
Protocol of screening PCR
Conditions of electrophoresis
Results
But we don't observe results for L102(3), L102(6), L103(4), L106(1), L106(2), L106(4), L111(1) Migration of an another gel for this sample...
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