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Team:Paris/August 12
From 2008.igem.org
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==New PCR screening with the right primers== | ==New PCR screening with the right primers== | ||
| - | Transformants with pFlgA, pFlgB and pFlhB cloned into J61002 are analysed by PCR but this time with the right primers (O18 and O19). | + | Transformants with pFlgA, pFlgB and pFlhB cloned into J61002 are analysed by PCR but this time with the right primers: '''VF2''' (O18) and '''VR''' (O19). |
*template: bacteria from glycerol stock | *template: bacteria from glycerol stock | ||
*positive control: J61002 (with ptet-mRFP) | *positive control: J61002 (with ptet-mRFP) | ||
Revision as of 13:10, 13 August 2008
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Minipreps: Plasmid extraction
Glycerol Stocks
Results of the transformations we did yesterdayConcentration of the MiniPreps
New PCR screening with the right primersTransformants with pFlgA, pFlgB and pFlhB cloned into J61002 are analysed by PCR but this time with the right primers: VF2 (O18) and VR (O19).
Electrophoresis
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