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Team:Paris/August 15
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AnaJimenez (Talk | contribs) (→Digestion) |
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| - | === | + | |
| + | ====Starting the construction of the Promoter characterization plasmid==== | ||
[[Team:Paris/Notebook/Protocols#Migration after digestion|standard protocol]] | [[Team:Paris/Notebook/Protocols#Migration after digestion|standard protocol]] | ||
| + | |||
| + | |||
| + | |||
| + | ===Digestion=== | ||
| + | |||
| + | ==Measurement of concentration of minipreps== | ||
| + | [[Team:Paris/Notebook/Protocols#Concentration of the Miniprep|standard protocol]] | ||
| + | |||
| + | {| border="1" style="text-align: center" | ||
| + | |Digestion | ||
| + | |Miniprep used | ||
| + | |Concentration (µg/mL) | ||
| + | |ratio 260/280 | ||
| + | |- | ||
| + | |D146 | ||
| + | |MP148.2 | ||
| + | |123 | ||
| + | |1.58 | ||
| + | |- | ||
| + | |D147 | ||
| + | |MP153.3 | ||
| + | |113 | ||
| + | |1.68 | ||
| + | |} | ||
| + | |||
| + | ==Digestion== | ||
| + | |||
| + | [[Team:Paris/Notebook/Protocols#Digestion|Protocol Digestion]] | ||
| + | |||
| + | {| border="1" style="text-align: center" | ||
| + | |Digestion | ||
| + | |Miniprep used | ||
| + | |Concentration (µg/mL) | ||
| + | |ratio 260/280 | ||
| + | |- | ||
| + | |D146 | ||
| + | |MP148.2 | ||
| + | |123 | ||
| + | |1.58 | ||
| + | |- | ||
| + | |D147 | ||
| + | |MP153.3 | ||
| + | |113 | ||
| + | |1.68 | ||
| + | |} | ||
==Analysis of yesterday PCR screening== | ==Analysis of yesterday PCR screening== | ||
Revision as of 15:51, 15 August 2008
Transformation of the ligations we did yesterdayWe transformed L 143, L 144 and the negative controls T1 and T2, using Invitrogen's TOP10 chemically competent cells
DigestionMeasurement of concentration of minipreps
Digestion
Starting the construction of the Promoter characterization plasmid
DigestionMeasurement of concentration of minipreps
Digestion
Analysis of yesterday PCR screening
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