Team:Paris/August 16

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=='''Ligation'''==
=='''Ligation'''==
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[[Team:Paris/Notebook/Protocols#Ligation |Transformation Ligation]]
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=='''Transformation'''==
=='''Transformation'''==

Revision as of 13:29, 17 August 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

Analysis of the transformation we did yesterday

L143, L144, T1 and T2 showed no colonies. The positive control with pUC19 worked well.
We suppose that the ligation did not work. We will do it again today.

Ligation

Transformation Ligation


Transformation

Transformation protocol

Name Description Antibio
Ligations
L150 D105(BV) - D146(BI)
pLas - strongest rbs-TetR-GFP tripart 		Amp
L151 D147(FI) - D125 (FV)
strongest rbs-LasR activator with LVA - Double terminator 		Kan
Controls
C1 D105 Amp
C2 D125 Kan
Positive Control pUC19 Amp
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