Team:Paris/August 17

From 2008.igem.org

(Difference between revisions)
(→Analysis of the transformation we did yesterday)
Line 49: Line 49:
|No
|No
|OK
|OK
 +
|}
 +
 +
==='''PCR Screening'''===
 +
[[Team:Paris/Protocols#PCR Screening| Protocol]]
 +
 +
==='''Minipreps'''===
 +
{|border="1" style="text-align: center"
 +
|'''Miniprep Name'''
 +
|'''Ligation name'''
 +
|''Antibio'''
 +
|'''Biobricks'''
 +
|'''Description'''
 +
|-
 +
|MP161.1
 +
|L150.1
 +
|rowspan="3" |Amp
 +
|rowspan="3" |[[Image:part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
 +
|rowspan="3" |pLas - rbs-TetR-GFP tripart
 +
|-
 +
|MP161.2
 +
|L150.2
 +
|-
 +
|MP161.3
 +
|L150.3
 +
|-
 +
|MP162.1
 +
|L151.1
 +
|rowspan="3" |Kan
 +
|rowspan="3" |[[Image:Part_icon_rbs.png]][[Image:icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
 +
|rowspan="3" |rbs-LasR - Double terminator
 +
|-
 +
|MP162.2
 +
|L151.2
 +
|-
 +
|MP162.3
 +
|L152.3
|}
|}

Revision as of 14:09, 17 August 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

Analysis of the transformation we did yesterday

Ligation name Description Antibio Number Colonies observed Fluorescence Comments
Ligations
L150 D105(BV) - D146(BI)
pLas - rbs-TetR-GFP Tripart 		Amp 3 Yes OK
L151 D147(FI) - D125(FV)
rbs-LasR - Double terminator 		Kan 21 No OK
Controls
C1 D105(BV) Amp 150 NO OK
C2 D125(FV) Kan 2 No OK
Positive Control pUC19 Amp 416 (efficiency 4.10^7) No OK

PCR Screening

Protocol

Minipreps

Miniprep Name Ligation name Antibio' Biobricks Description
MP161.1 L150.1 Amp Part icon regulatory.pngPart icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png pLas - rbs-TetR-GFP tripart
MP161.2 L150.2
MP161.3 L150.3
MP162.1 L151.1 Kan Part icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png rbs-LasR - Double terminator
MP162.2 L151.2
MP162.3 L152.3
Retrieved from "http://2008.igem.org/Team:Paris/August_17"