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Team:Paris/August 17
From 2008.igem.org
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Remarks: | Remarks: | ||
L143 and L144 did not work once again. Maybe there is a problem with the digestion, because the primers used present only two | L143 and L144 did not work once again. Maybe there is a problem with the digestion, because the primers used present only two | ||
| - | nucleotides after the restriction sites. what we should do is cutting pfliL with xbaI and SpeI. As the vector will autoligate, we should use a phosphatase to prevent this phenomenon. | + | nucleotides after the restriction sites. what we should do is cutting pfliL with xbaI and SpeI. As the vector will autoligate, |
| + | we should use a phosphatase to prevent this phenomenon. | ||
For L147 and its control (T4), we do not observe any red fluorescence. | For L147 and its control (T4), we do not observe any red fluorescence. | ||
It means that the digestion work well, because if it was not digested, the strong promoter J23100 should express mRFP! | It means that the digestion work well, because if it was not digested, the strong promoter J23100 should express mRFP! | ||
Revision as of 15:59, 17 August 2008
Analysis of the transformation we did yesterday
PCR Screening==> Protocol Minipreps
Analysis of the other transformation we did yesterdayEvaluation of the number of colonies
Remarks:
L143 and L144 did not work once again. Maybe there is a problem with the digestion, because the primers used present only two
nucleotides after the restriction sites. what we should do is cutting pfliL with xbaI and SpeI. As the vector will autoligate,
we should use a phosphatase to prevent this phenomenon.
For L147 and its control (T4), we do not observe any red fluorescence.
It means that the digestion work well, because if it was not digested, the strong promoter J23100 should express mRFP!
We will do the screening anyway.
PCR ScreeningMiniprepsKok-Phen transformation we did yesterday |
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