Team:Paris/August 20
From 2008.igem.org
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(→Construction of pFlgA - YFP tripart) |
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The plates obtained from the speading of yesterday can't be used because there are not single colonies. | The plates obtained from the speading of yesterday can't be used because there are not single colonies. | ||
<br>We have to try again, but with a stronger dilution of the bacteria or with a smaller volume of spreading. | <br>We have to try again, but with a stronger dilution of the bacteria or with a smaller volume of spreading. | ||
- | + | <br>Some bacteria from the glycerol stock were resuspend into 1 mL of LB+antibiotic, then we made a dilution 10 and a dilution 100. | |
+ | <br>Each of the dilution was spread on a LB plate containing the right antibiotic. | ||
+ | <br>Overnight incubation | ||
=Construction of pFlgA - YFP tripart= | =Construction of pFlgA - YFP tripart= |
Revision as of 17:22, 20 August 2008
Screening of the cloning of E0240 and FlhDC+promotorSpreading the clones in order to obtain single colonies
The plates obtained from the speading of yesterday can't be used because there are not single colonies.
Construction of pFlgA - YFP tripartAim : Construction of "pFlgA-RBS-YFP-dbl ter" (pFlgA-E0430/E0432) DigestionMeasurement of concentration of minipreps
Digestion
Gel Extraction
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