Team:Paris/July 23
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{{Paris/Calendar_Links|July 22|July 24}} | {{Paris/Calendar_Links|July 22|July 24}} | ||
- | == | + | ==Glycerol Stocks== |
- | *1mL of each culture (with 2 clones) has been added to 1mL of | + | *1mL of each culture (with 2 clones) has been added to 1mL of 40% glycerol. |
- | *For each clone, two | + | *For each clone, two glycerol stocks have been done. |
*Stored at -20°C. | *Stored at -20°C. | ||
- | |||
== MiniPreps == | == MiniPreps == | ||
- | The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 22th. | + | *The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 22th. |
- | + | ||
{| border="1" | {| border="1" | ||
|align="center"|'''Name''' | |align="center"|'''Name''' | ||
|align="center"|'''Plasmid''' | |align="center"|'''Plasmid''' | ||
- | |align="center"|''' | + | |align="center"|'''Description''' |
|- | |- | ||
|align="center"|MP100 | |align="center"|MP100 | ||
|align="center"|B0034 | |align="center"|B0034 | ||
- | |align="center"|Srtongest RBS | + | |align="center"|Srtongest RBS (affinity=1) |
|- | |- | ||
Line 32: | Line 30: | ||
|align="center"|MP103 | |align="center"|MP103 | ||
|align="center"|R0079 | |align="center"|R0079 | ||
- | |align="center"| | + | |align="center"|plas |
- | + | ||
|- | |- | ||
+ | |align="center"|MP104 | ||
+ | |align="center"|R0040 | ||
+ | |align="center"|pTet | ||
+ | |- | ||
+ | |align="center"|MP105 | ||
+ | |align="center"|S03154 | ||
+ | |align="center"|RBS-lasI | ||
+ | |- | ||
+ | |align="center"|MP106 | ||
+ | |align="center"|S03879 | ||
+ | |align="center"|RBS-TetR | ||
+ | |- | ||
+ | |align="center"|MP107 | ||
+ | |align="center"|C0079 | ||
+ | |align="center"|LasR LVAtag | ||
+ | |- | ||
+ | |align="center"|MP108 | ||
+ | |align="center"|C0179 | ||
+ | |align="center"|LasR | ||
+ | |- | ||
+ | |align="center"|MP109 | ||
|align="center"|E0030 | |align="center"|E0030 | ||
- | |||
|align="center"|YFP | |align="center"|YFP | ||
- | |||
|- | |- | ||
+ | |align="center"|MP110 | ||
|align="center"|E0040 | |align="center"|E0040 | ||
- | |||
|align="center"|GFP | |align="center"|GFP | ||
- | |||
|- | |- | ||
+ | |align="center"|MP111 | ||
|align="center"|E1010 | |align="center"|E1010 | ||
- | |align="center"| | + | |align="center"|RFP |
- | + | ||
- | + | ||
|- | |- | ||
- | |align="center"| | + | |align="center"|MP112 |
- | |align="center"| | + | |align="center"| |
- | |align="center"| | + | |align="center"|OmpR M57I |
- | + | ||
|- | |- | ||
- | |align="center"| | + | |align="center"|MP113 |
- | |align="center"| | + | |align="center"| |
- | |align="center"| | + | |align="center"|EnvZ I281S |
- | + | ||
|- | |- | ||
- | |align="center"| | + | |align="center"|MP114 |
- | |align="center"| | + | |align="center"|C0040 |
- | |align="center"| | + | |align="center"|Tetracyclin repressor |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
|- | |- | ||
+ | |align="center"|MP115 | ||
|align="center"|pSB1A3 | |align="center"|pSB1A3 | ||
- | |align="center"| | + | |align="center"|High copy number plasmid |
- | + | ||
- | + | ||
|- | |- | ||
- | |align="center"| | + | |align="center"|MP122 |
- | |align="center"| | + | |align="center"|E0840 |
- | |align="center"| | + | |align="center"|GFP tripart |
- | |align="center"| | + | |} |
+ | |||
+ | == Cultures == | ||
+ | *2007 strains have been taken and put in 6mL of LB with the adequat antibiotic. | ||
+ | |||
+ | {| border="1" | ||
+ | |align="center"|'''Name''' | ||
+ | |align="center"|'''Biobricks''' | ||
+ | |align="center"|'''Description''' | ||
|- | |- | ||
- | |align="center"| | + | |align="center"|S4 |
- | |align="center"| | + | |align="center"|J23100 |
- | |align="center"| | + | |align="center"|strong constitutive promoter |
- | |align="center"| | + | |- |
+ | |align="center"|S5 | ||
+ | |align="center"|B0015 | ||
+ | |align="center"|double terminator | ||
+ | |- | ||
+ | |align="center"|S7 | ||
+ | |align="center"|B0030 | ||
+ | |align="center"|strong RBS : 0,6 | ||
+ | |- | ||
+ | |align="center"|S8 | ||
+ | |align="center"|E0422 | ||
+ | |align="center"|ECFP tripart | ||
+ | |- | ||
+ | |align="center"|S13 | ||
+ | |align="center"|I0500 | ||
+ | |align="center"|pBAD | ||
+ | |- | ||
+ | |align="center"|S14 | ||
+ | |align="center"|J23107 | ||
+ | |align="center"|Medium constitutive promoter | ||
|} | |} |
Latest revision as of 10:15, 25 August 2008
Glycerol Stocks
MiniPreps
Cultures
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