Team:Paris/August 26
From 2008.igem.org
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(→PCR3) |
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'''''Cycling 2 (30X) :''''' <br> | '''''Cycling 2 (30X) :''''' <br> | ||
98°C-10"<br> | 98°C-10"<br> | ||
+ | 72°C-30"<br> | ||
+ | '''''Elongation :''''' <br> | ||
+ | 72°C-5'<br> | ||
+ | |||
+ | '''Program: PCRFliA3'''<br> | ||
+ | '''''Denaturation :''''' <br> | ||
+ | 98°C-30'<br> | ||
+ | '''''Cycling 1 (2X) :''''' <br> | ||
+ | 98°C-10"<br> | ||
+ | 72°C-30"<br> | ||
+ | '''''Cycling 2 (5X) :''''' <br> | ||
+ | 98°C-10"<br> | ||
+ | 98°C->72°C low decreasing 1.0°C/s<br> | ||
72°C-30"<br> | 72°C-30"<br> | ||
'''''Elongation :''''' <br> | '''''Elongation :''''' <br> |
Revision as of 11:16, 26 August 2008
PCR1
Program: Gradient Vf=20µL PCR2
Program: promoter Vf=50µL PCR3
Program: PCRFliA1 Program: PCRFliA2 Program: PCRFliA3 Cloning of EnvZ*Concentration measurement by Biophotometer
Ligation |