Team:Paris/August 26
From 2008.igem.org
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(→PCR) |
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Phusion=0,5µL<br> | Phusion=0,5µL<br> | ||
+ | ==PCR3== | ||
+ | * PCRFliA1 (O143-F / O152-R) | ||
+ | * PCRFliA2 (O153-F / O150-R) | ||
+ | * PCRFliA3 (O148-F / O150-R) | ||
+ | |||
+ | ---- | ||
+ | |||
+ | '''Program: PCRFliA1'''<br> | ||
+ | '''''Denaturation :''''' <br> | ||
+ | 98°C-5'<br> | ||
+ | '''''Cycling 1 (30X) :''''' <br> | ||
+ | 98°C-10"<br> | ||
+ | Gradient 66°C +/-6°C - 25''<br> | ||
+ | 72°C-20"<br> | ||
+ | '''''Elongation :''''' <br> | ||
+ | 72°C-5'<br> | ||
+ | |||
+ | '''Program: PCRFliA2'''<br> | ||
+ | '''''Denaturation :''''' <br> | ||
+ | 98°C-5'<br> | ||
+ | '''''Cycling 1 (3X) :''''' <br> | ||
+ | 98°C-10"<br> | ||
+ | Gradient 61°C +/-10°C - 25''<br> | ||
+ | 72°C-20"<br> | ||
+ | '''''Cycling 2 (30X) :''''' <br> | ||
+ | 98°C-10"<br> | ||
+ | 72°C-30"<br> | ||
+ | '''''Elongation :''''' <br> | ||
+ | 72°C-5'<br> | ||
+ | |||
+ | '''Program: PCRFliA3'''<br> | ||
+ | '''''Denaturation :''''' <br> | ||
+ | 98°C-30'<br> | ||
+ | '''''Cycling 1 (2X) :''''' <br> | ||
+ | 98°C-10"<br> | ||
+ | 72°C-30"<br> | ||
+ | '''''Cycling 2 (5X) :''''' <br> | ||
+ | 98°C-10"<br> | ||
+ | 98°C->72°C low decreasing 1.0°C/s<br> | ||
+ | 72°C-30"<br> | ||
+ | '''''Elongation :''''' <br> | ||
+ | 72°C-5'<br> | ||
=Cloning of EnvZ*= | =Cloning of EnvZ*= |
Revision as of 11:18, 26 August 2008
PCRPCR1
Program: Gradient Vf=20µL PCR2
Program: promoter Vf=50µL PCR3
Program: PCRFliA1 Program: PCRFliA2 Program: PCRFliA3 Cloning of EnvZ*Concentration measurement by Biophotometer
Ligation |