Team:Paris/August 29

From 2008.igem.org

(Difference between revisions)
(→Cloning of EnvZ*)
Line 4: Line 4:
==Concentration measurement==
==Concentration measurement==
 +
 +
{|border="1" style="text-align: center"
 +
|
 +
|DNA concentration
 +
|A260 / A2801
 +
|-
 +
|D159 (EnvZ*)
 +
|9 µg/mL
 +
|1,59
 +
|D116 (pSB1A2)
 +
|13 µg/mL
 +
|1,29
 +
|}
==Ligation==
==Ligation==
 +
 +
{|border="1" style="text-align: center"
 +
|rowspan="2"|
 +
|rowspan="2"|control
 +
|colspan="2"|insert / vector ratio
 +
|-
 +
|3 / 1
 +
|4 / 1
 +
|-
 +
|D159 (EnvZ*)
 +
|0
 +
|8,7 µL
 +
|11,6 µL
 +
|-
 +
|D116 (pSB1A2)
 +
|2 µL
 +
|2 µL
 +
|2 µL
 +
|-
 +
|T4 DNA ligase 10X buffer
 +
|2 µL
 +
|2 µL
 +
|2 µL
 +
|-
 +
|T4 DNA ligase
 +
|1 µL
 +
|1 µL
 +
|1 µL
 +
|-
 +
|H2O (qsp 20 µL)
 +
|15 µL
 +
|6,3 µL
 +
|3,4 µL
 +
|}

Revision as of 12:59, 29 August 2008

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Cloning of EnvZ*

Concentration measurement

DNA concentration A260 / A2801
D159 (EnvZ*) 9 µg/mL 1,59 D116 (pSB1A2) 13 µg/mL 1,29

Ligation

control insert / vector ratio
3 / 1 4 / 1
D159 (EnvZ*) 0 8,7 µL 11,6 µL
D116 (pSB1A2) 2 µL 2 µL 2 µL
T4 DNA ligase 10X buffer 2 µL 2 µL 2 µL
T4 DNA ligase 1 µL 1 µL 1 µL
H2O (qsp 20 µL) 15 µL 6,3 µL 3,4 µL
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