Team:Paris/August 29

From 2008.igem.org

(Difference between revisions)
(→Cloning of EnvZ*)
(→Cloning of EnvZ*)
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|colspan="2"|'''insert / vector ratio'''
|colspan="2"|'''insert / vector ratio'''
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|-
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|3 / 1
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|'''3 / 1'''
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|4 / 1
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|'''4 / 1'''
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|D159 (EnvZ*)
|D159 (EnvZ*)

Revision as of 13:12, 29 August 2008

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Cloning of EnvZ*

Concentration measurement

DNA concentration A260 / A2801
D159 (EnvZ*) 9 µg/mL 1,59
D116 (pSB1A2) 13 µg/mL 1,29

Ligation

control insert / vector ratio
3 / 1 4 / 1
D159 (EnvZ*) 0 8,7 µL 11,6 µL
D116 (pSB1A2) 2 µL 2 µL 2 µL
T4 DNA ligase 10X buffer 2 µL 2 µL 2 µL
T4 DNA ligase 1 µL 1 µL 1 µL
H2O (qsp 20 µL) 15 µL 6,3 µL 3,4 µL
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