Team:Paris/September 5

From 2008.igem.org

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=Purification of yesterday digestion=
=Purification of yesterday digestion=
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[[Image:KR000300.JPG|thumb|D112 before gel excision]]
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[[Image:KR000301.JPG|thumb|After gel excision]]
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==Electrophoresis and gel extraction==
==Electrophoresis and gel extraction==
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[[Image:KR000300.JPG|thumb|D112 before gel excision]]
 
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[[Image:KR000301.JPG|thumb|after gel excision]]
 
1% agarose gel
1% agarose gel

Revision as of 16:08, 5 September 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

Purification of yesterday digestion

D112 before gel excision
After gel excision
Digestion n° DNA substrat Digestion by
D112 MP106: S03879 (rbs-TetR) in pSB1A2 EcoRI & SpeI

Electrophoresis and gel extraction

1% agarose gel

Expected size Measured size
pSB1A2 (vector) 2079 bp 2 kb
rbs-TetR (insert) 703 bp 0,7 kb

Qiaquick Gel Extraction Kit

  • elution in 30 µL of EB buffer

Concentration measurement

Sample DNA concentration A260/A280
D112 8 µg/mL 2,31
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