Team:Paris/September 1

From 2008.igem.org

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='''Construction of pFlhB - mRFP Tripart (LVA+)'''=
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Aim : Construction of ''' "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078)''' [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
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<br>We can only do the construction with mRFP Tripart (LVA+) because the stable strain with the Biobricks I732011 (mRFP Tripart LVA-) don't to growth.
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==Digestion==
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==='''Measurement of the concentration of D187 purified'''===
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[[Team:Paris/Notebook/Protocols#Concentration_of_the_Miniprep | Protocol (it's same that for Miniprep)]]
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{|border="1" style="text-align: center"
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|'''Digestion Name'''
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|'''Concentration (µg/mL)'''
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|'''Ratio 260/280'''
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|-
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|D187
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|16
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|1.31
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|}
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==Ligation==
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[[Team:Paris/Notebook/Protocols#Ligation |Protocol]]
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{|border="1" style="text-align: center"
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|'''Ligation Name'''
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|'''Vector Name'''
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|'''Volume Vector (µL)'''
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|'''Insert'''
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|'''Volume Insert (µL)'''
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|-
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|L168
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|D187
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|
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|D186
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|
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|-
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|Control L168
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|D166
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|
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| -
|}
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Revision as of 19:00, 9 September 2008

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Contents

Cloning of EnvZ*

Transformation results of L165 (EnvZ*-pSB1A2) from August 29th

sample number of colonies
positive control pUC19 many colonies
transformation negative control 0
ligation without insert 0
3:1 insert:vector ratio 5
4:1 insert / vector ratio 5

Construction of pFlhB - mRFP Tripart (LVA+)

Aim : Construction of "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078) Part icon regulatory.pngPart icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
We can only do the construction with mRFP Tripart (LVA+) because the stable strain with the Biobricks I732011 (mRFP Tripart LVA-) don't to growth.

Digestion

Measurement of the concentration of D187 purified

Protocol (it's same that for Miniprep)

Digestion Name Concentration (µg/mL) Ratio 260/280
D187 16 1.31

Ligation

Protocol

Ligation Name Vector Name Volume Vector (µL) Insert Volume Insert (µL)
L168 D187 D186
Control L168 D166 -
Retrieved from "http://2008.igem.org/Team:Paris/September_1"