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Team:Paris/September 11
From 2008.igem.org
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=Checking our ligases= | =Checking our ligases= | ||
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| + | Because we couldn't be sure of the results of yesterday experiment, we decided to carry it out one more time. | ||
==Digestion== | ==Digestion== | ||
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[[Image:Before-excision.JPG|thumb|Before excision]] | [[Image:Before-excision.JPG|thumb|Before excision]] | ||
[[Image:After-excision.JPG|thumb|After ecxision]] | [[Image:After-excision.JPG|thumb|After ecxision]] | ||
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| + | DNA used for digestion: MP101 | ||
| + | <br> 2 digestion were carried out: | ||
| + | *one with EcoRI | ||
| + | *the other with BspHI | ||
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==Purification== | ==Purification== | ||
==Ligation== | ==Ligation== | ||
Revision as of 10:39, 12 September 2008
Checking our ligasesBecause we couldn't be sure of the results of yesterday experiment, we decided to carry it out one more time. Digestion  DNA used for digestion: MP101
PurificationLigation |
