Team:Paris/September 1

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(→Construction of pFlhB - mRFP Tripart (LVA+))
 
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|'''number of colonies'''
|'''number of colonies'''
|-
|-
-
|positive control pUC19
+
|'''positive''' control pUC19
|many colonies
|many colonies
|-
|-
-
|transformation negative control
+
|transformation '''negative''' control
|0
|0
|-
|-
-
|ligation without insert
+
|ligation '''without insert'''
|0
|0
|-
|-
-
|3:1 insert:vector ratio
+
|'''3:1''' insert:vector ratio
|5
|5
|-
|-
-
|4:1 insert / vector ratio
+
|'''4:1''' insert / vector ratio
|5
|5
|-
|-
 +
|}
 +
 +
 +
=Construction of rbs-FlhDC-mRFP-LVA-tripart (L172)=
 +
 +
Aim : Construction of ''' "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078)''' [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
 +
<br>==> it appears a mistake of name digestion, it explains the realisation of a construction not wanted
 +
 +
==Digestion==
 +
==='''Measurement of the concentration of D187 purified'''===
 +
 +
[[Team:Paris/Notebook/Protocols#Concentration_of_the_Miniprep | Protocol (it's same that for Miniprep)]]
 +
 +
{|border="1" style="text-align: center"
 +
|'''Digestion Name'''
 +
|'''Concentration (µg/mL)'''
 +
|'''Ratio 260/280'''
 +
|-
 +
|D187
 +
|16
 +
|1.31
 +
|-
 +
|D186
 +
|24
 +
|1.25
 +
|}
 +
 +
==Ligation==
 +
[[Team:Paris/Notebook/Protocols#Ligation |Protocol]]
 +
 +
{|border="1" style="text-align: center"
 +
|'''Ligation Name'''
 +
|'''Vector Name'''
 +
|'''Volume Vector (µL)'''
 +
|'''Insert'''
 +
|'''Volume Insert (µL)'''
 +
|-
 +
|L168
 +
|D187
 +
|2.5
 +
|D186
 +
|1
 +
|-
 +
|Control L168
 +
|D166
 +
|2.5
 +
| -
|}
|}

Latest revision as of 14:49, 13 September 2008

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Contents

Cloning of EnvZ*

Transformation results of L165 (EnvZ*-pSB1A2) from August 29th

sample number of colonies
positive control pUC19 many colonies
transformation negative control 0
ligation without insert 0
3:1 insert:vector ratio 5
4:1 insert / vector ratio 5


Construction of rbs-FlhDC-mRFP-LVA-tripart (L172)

Aim : Construction of "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078) Part icon regulatory.pngPart icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
==> it appears a mistake of name digestion, it explains the realisation of a construction not wanted

Digestion

Measurement of the concentration of D187 purified

Protocol (it's same that for Miniprep)

Digestion Name Concentration (µg/mL) Ratio 260/280
D187 16 1.31
D186 24 1.25

Ligation

Protocol

Ligation Name Vector Name Volume Vector (µL) Insert Volume Insert (µL)
L168 D187 2.5 D186 1
Control L168 D166 2.5 -
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