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TUDelft/15 September 2008
From 2008.igem.org
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{{Template:TUDelftiGEM2008_calendar}} | {{Template:TUDelftiGEM2008_calendar}} | ||
| - | =September 15th | + | =September 15th= |
==Transformation== | ==Transformation== | ||
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==Induction== | ==Induction== | ||
| - | To test for R0080 function, we're trying to visualize luciferase expression on an SDS-PAGE. Therefore we made a 1.33 M arabinose stock (1000x) for induction and have grown [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115012 K115012] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115011 K115011] o/n both with and without induction. | + | To test for R0080 function, we're trying to visualize luciferase expression on an SDS-PAGE. Therefore we made a 1.33 M arabinose stock (1000x) for induction [http://tools.invitrogen.com/content/sfs/brochures/710_01619_pBAD_bro.pdf] (page 3) and have grown [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115012 K115012] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115011 K115011] o/n both with and without induction. |
| + | |||
| + | ==Colony PCR ''E. coli'' genes== | ||
| + | A colony PCR on eight colonies for atoB, idi and ispA were performed. The PCR was performed o/n, after the program the machine was programmed to keep a constant temperature of 4 degrees Celsius. The result will be checked on gel tomorrow. | ||
{{Template:TUDelftiGEM2008_sidebar}} | {{Template:TUDelftiGEM2008_sidebar}} | ||
Latest revision as of 10:59, 27 October 2008
[http://www.tudelft.nl ]
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| [http://2008.igem.org/TUDelft/1_September_2008 1] | [http://2008.igem.org/TUDelft/2_September_2008 2] | [http://2008.igem.org/TUDelft/3_September_2008 3] | [http://2008.igem.org/TUDelft/4_September_2008 4] | [http://2008.igem.org/TUDelft/5_September_2008 5] | [http://2008.igem.org/wiki/index.php?title=TUDelft/6_September_2008&action=edit 6] | [http://2008.igem.org/wiki/index.php?title=TUDelft/7_September_2008&action=edit 7] |
| [http://2008.igem.org/TUDelft/8_September_2008 8] | [http://2008.igem.org/TUDelft/9_September_2008 9] | [http://2008.igem.org/TUDelft/10_September_2008 10] | [http://2008.igem.org/TUDelft/11_September_2008 11] | [http://2008.igem.org/TUDelft/12_September_2008 12] | [http://2008.igem.org/wiki/index.php?title=TUDelft/13_September_2008&action=edit 13] | [http://2008.igem.org/wiki/index.php?title=TUDelft/14_September_2008&action=edit 14] |
| [http://2008.igem.org/TUDelft/15_September_2008 15] | [http://2008.igem.org/TUDelft/16_September_2008 16] | [http://2008.igem.org/TUDelft/17_September_2008 17] | [http://2008.igem.org/TUDelft/18_September_2008 18] | [http://2008.igem.org/TUDelft/19_September_2008 19] | [http://2008.igem.org/wiki/index.php?title=TUDelft/20_September_2008&action=edit 20] | [http://2008.igem.org/wiki/index.php?title=TUDelft/21_September_2008&action=edit 21] |
| [http://2008.igem.org/TUDelft/22_September_2008 22] | [http://2008.igem.org/TUDelft/23_September_2008 23] | [http://2008.igem.org/TUDelft/24_September_2008 24] | [http://2008.igem.org/TUDelft/25_September_2008 25] | [http://2008.igem.org/wiki/index.php?title=TUDelft/26_September_2008&action=edit 26] | [http://2008.igem.org/wiki/index.php?title=TUDelft/27_September_2008&action=edit 27] | [http://2008.igem.org/wiki/index.php?title=TUDelft/28_September_2008&action=edit 28] |
| [http://2008.igem.org/TUDelft/29_September_2008 29] | [http://2008.igem.org/TUDelft/30_September_2008 30] | |||||
| October | ||||||
| M | T | W | T | F | S | S |
| [http://2008.igem.org/TUDelft/1_October_2008 1] | [http://2008.igem.org/TUDelft/2_October_2008 2] | [http://2008.igem.org/TUDelft/3_October_2008 3] | [http://2008.igem.org/wiki/index.php?title=TUDelft/4_October_2008&action=edit 4] | [http://2008.igem.org/wiki/index.php?title=TUDelft/5_October_2008&action=edit 5] | ||
| [http://2008.igem.org/TUDelft/6_October_2008 6] | [http://2008.igem.org/TUDelft/7_October_2008 7] | [http://2008.igem.org/TUDelft/8_October_2008 8] | [http://2008.igem.org/TUDelft/9_October_2008 9] | [http://2008.igem.org/TUDelft/10_October_2008 10] | [http://2008.igem.org/wiki/index.php?title=TUDelft/11_October_2008&action=edit 11] | [http://2008.igem.org/wiki/index.php?title=TUDelft/12_October_2008&action=edit 12] |
| [http://2008.igem.org/TUDelft/13_October_2008 13] | [http://2008.igem.org/TUDelft/14_October_2008 14] | [http://2008.igem.org/TUDelft/15_October_2008 15] | [http://2008.igem.org/TUDelft/16_October_2008 16] | [http://2008.igem.org/TUDelft/17_October_2008 17] | [http://2008.igem.org/wiki/index.php?title=TUDelft/18_October_2008&action=edit 18] | [http://2008.igem.org/wiki/index.php?title=TUDelft/19_October_2008&action=edit 19] |
| [http://2008.igem.org/TUDelft/20_October_2008 20] | [http://2008.igem.org/TUDelft/21_October_2008 21] | [http://2008.igem.org/TUDelft/22_October_2008 22] | [http://2008.igem.org/TUDelft/23_October_2008 23] | [http://2008.igem.org/TUDelft/24_October_2008 24] | [http://2008.igem.org/wiki/index.php?title=TUDelft/25_October_2008&action=edit 25] | [http://2008.igem.org/wiki/index.php?title=TUDelft/26_October_2008&action=edit 26] |
| [http://2008.igem.org/wiki/index.php?title=TUDelft/27_October_2008&action=edit 27] | [http://2008.igem.org/wiki/index.php?title=TUDelft/28_October_2008&action=edit 28] | [http://2008.igem.org/wiki/index.php?title=TUDelft/29_October_2008&action=edit 29] | [http://2008.igem.org/wiki/index.php?title=TUDelft/30_October_2008&action=edit 30] | [http://2008.igem.org/wiki/index.php?title=TUDelft/31_October_2008&action=edit 31] | ||
Contents |
September 15th
Transformation
The DNA ligation over the weekend has been transformed into TOP10 made chemically competent.
Induction
To test for R0080 function, we're trying to visualize luciferase expression on an SDS-PAGE. Therefore we made a 1.33 M arabinose stock (1000x) for induction [http://tools.invitrogen.com/content/sfs/brochures/710_01619_pBAD_bro.pdf] (page 3) and have grown [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115012 K115012] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115011 K115011] o/n both with and without induction.
Colony PCR E. coli genes
A colony PCR on eight colonies for atoB, idi and ispA were performed. The PCR was performed o/n, after the program the machine was programmed to keep a constant temperature of 4 degrees Celsius. The result will be checked on gel tomorrow.
