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TUDelft/15 October 2008
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==Luciferase and protein content measurements== | ==Luciferase and protein content measurements== | ||
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| + | The results of the corrected luciferase/total protein activity is displayed in figure 1. Unfortunately, all the strains grown at 25ºC and 30ºC showed no luciferase activity. We expect lysis of the cells did not work, due to a low amplitude of the sonicator. K115031 is left out here because it has such a high luciferase activity it interferes with the scale. For the full graph click [[TUDelft/081015c.png|here]]. | ||
| + | |||
| + | [[Image:TUDelft221508a.png|thumb|center|400px|Figure 1: Corrected luciferase activity in lum / mg total protein. Errorbars represent N=4. Luciferase measured in duplo.]] | ||
| + | |||
| + | In figure 2 we displayed the 37ºC fold increase of luminescence / total protein over the 20ºC sample. | ||
| + | [[Image:TUDelft221508b.png|thumb|center|400px|Figure 2: Fold increase of luminescence / total protein over the 20ºC sample (normalized for 20ºC).]] | ||
| + | |||
| + | From this experiment we drew the conclusion that construct K115035 is the most probable construct for showing temperature induced switching behavior. | ||
| + | |||
| + | ==Growing 25ºC and 30ºC cultures (again)== | ||
| + | |||
| + | Due to the failing of lysis of cultures grown at these temperatures, we've started new o/n growth of these cultures. New method of lysis will be the Fastprep machine. | ||
{{Template:TUDelftiGEM2008_sidebar}} | {{Template:TUDelftiGEM2008_sidebar}} | ||
Latest revision as of 15:09, 28 October 2008
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| [http://2008.igem.org/wiki/index.php?title=TUDelft/27_October_2008&action=edit 27] | [http://2008.igem.org/wiki/index.php?title=TUDelft/28_October_2008&action=edit 28] | [http://2008.igem.org/wiki/index.php?title=TUDelft/29_October_2008&action=edit 29] | [http://2008.igem.org/wiki/index.php?title=TUDelft/30_October_2008&action=edit 30] | [http://2008.igem.org/wiki/index.php?title=TUDelft/31_October_2008&action=edit 31] | ||
October 15th
Luciferase and protein content measurements
The results of the corrected luciferase/total protein activity is displayed in figure 1. Unfortunately, all the strains grown at 25ºC and 30ºC showed no luciferase activity. We expect lysis of the cells did not work, due to a low amplitude of the sonicator. K115031 is left out here because it has such a high luciferase activity it interferes with the scale. For the full graph click here.
In figure 2 we displayed the 37ºC fold increase of luminescence / total protein over the 20ºC sample.
From this experiment we drew the conclusion that construct K115035 is the most probable construct for showing temperature induced switching behavior.
Growing 25ºC and 30ºC cultures (again)
Due to the failing of lysis of cultures grown at these temperatures, we've started new o/n growth of these cultures. New method of lysis will be the Fastprep machine.
